TY - JOUR
T1 - Natural sporopollenin microcapsules
T2 - biological evaluation and application in regulating hepatic toxicity of diclofenac sodium in vivo
AU - Meligi, Noha M.
AU - Dyab, Amro K.F.
N1 - Publisher Copyright:
© 2023 The Royal Society of Chemistry.
PY - 2023/7/17
Y1 - 2023/7/17
N2 - Diclofenac sodium (DIC) is a pain reliever and anti-nociceptive medication. Significant limitations of DIC treatment stem from its adverse effects. This study investigates the feasibility of using natural Lycopodium clavatum sporopollenin (LCS) microcapsules loaded with DIC to mitigate the hepatotoxicity associated with DIC treatment. In addition, LCS microcapsules were tracked in the blood, stomach, small intestine, and feces of rats to demonstrate their morphological integrity and uptake behavior. Four groups (6 per group) of adult male albino rats were administered normal saline (control), empty LCS (30 mg kg−1), plain DIC (10 mg kg−1), and DIC-loaded LCS (40 mg kg−1) orally for seven consecutive days. The first comprehensive histological examination of the rat stomach demonstrated the robustness and bioadhesion ability of LCS under severe conditions. The findings suggested that these versatile microcapsules are unlikely to be digested in the gastrointestinal tract (GIT). The administration of DIC-loaded LCS was found to play a potential protective role in regulating DIC-induced substantially increased serum levels of transaminases, alkaline phosphatase, total bilirubin, and pro-inflammatory cytokines. In addition, DIC-loaded LCS restored the antioxidant enzymes, DNA damage, and liver histological architecture abnormalities caused by DIC. Microencapsulation of DIC into pollen-derived biomaterials could be employed as an efficient platform with enough safety coverage on rat liver, pending further clinical studies.
AB - Diclofenac sodium (DIC) is a pain reliever and anti-nociceptive medication. Significant limitations of DIC treatment stem from its adverse effects. This study investigates the feasibility of using natural Lycopodium clavatum sporopollenin (LCS) microcapsules loaded with DIC to mitigate the hepatotoxicity associated with DIC treatment. In addition, LCS microcapsules were tracked in the blood, stomach, small intestine, and feces of rats to demonstrate their morphological integrity and uptake behavior. Four groups (6 per group) of adult male albino rats were administered normal saline (control), empty LCS (30 mg kg−1), plain DIC (10 mg kg−1), and DIC-loaded LCS (40 mg kg−1) orally for seven consecutive days. The first comprehensive histological examination of the rat stomach demonstrated the robustness and bioadhesion ability of LCS under severe conditions. The findings suggested that these versatile microcapsules are unlikely to be digested in the gastrointestinal tract (GIT). The administration of DIC-loaded LCS was found to play a potential protective role in regulating DIC-induced substantially increased serum levels of transaminases, alkaline phosphatase, total bilirubin, and pro-inflammatory cytokines. In addition, DIC-loaded LCS restored the antioxidant enzymes, DNA damage, and liver histological architecture abnormalities caused by DIC. Microencapsulation of DIC into pollen-derived biomaterials could be employed as an efficient platform with enough safety coverage on rat liver, pending further clinical studies.
UR - http://www.scopus.com/inward/record.url?scp=85167513466&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85167513466&partnerID=8YFLogxK
U2 - 10.1039/d3bm00638g
DO - 10.1039/d3bm00638g
M3 - Article
C2 - 37522344
AN - SCOPUS:85167513466
SN - 2047-4830
VL - 11
SP - 6193
EP - 6209
JO - Biomaterials Science
JF - Biomaterials Science
IS - 18
ER -