Non-deleterious Dinitrophenyl (DNP) hapten labelling of antibody protein. Preparation and properties of some short-chain DNP imidoesters

Michael J.E. Hewlins, Ian Weeks, Bharat Jasani

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Methyl 3-(2,4-dinitrophenylamino)propionimidate hydrochloride (DNP-N-IE), methyl 3-(2,4-dinitrophenyl-N-methylamino) propionimidate hydrochloride (DNP-NMe-IE), and methyl 3-(2,4-dinitrophenylthio)propionimidate hydrochloride (DNP-S-IE) have been prepared. DNP-N-IE and DNP-NMe-IE both react efficiently at 0-2°C and pH 7-9.5 with sheep immunoglobulin G. At least 12 DNP groups can be introduced into the antibody protein without causing any significant change in its antigen binding affinity or capacity.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalJournal of Immunological Methods
Volume70
Issue number1
DOIs
Publication statusPublished - May 11 1984

Keywords

  • antibody labeling
  • antigen binding affinity
  • antigen binding capacity
  • dinitrophenyl (DNP)
  • hapten
  • imidoester

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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