Noneluting enzymatic antibiofilm coatings

Svetlana V. Pavlukhina, Jeffrey B. Kaplan, Li Xu, Wei Chang, Xiaojun Yu, Srinivasa Madhyastha, Nandadeva Yakandawala, Almagul Mentbayeva, Babar Khan, Svetlana A. Sukhishvili

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We developed a highly efficient, biocompatible surface coating that disperses bacterial biofilms through enzymatic cleavage of the extracellular biofilm matrix. The coating was fabricated by binding the naturally existing enzyme dispersin B (DspB) to surface-attached polymer matrices constructed via a layer-by-layer (LbL) deposition technique. LbL matrices were assembled through electrostatic interactions of poly(allylamine hydrochloride) (PAH) and poly(methacrylic acid) (PMAA), followed by chemical cross-linking with glutaraldehyde and pH-triggered removal of PMAA, producing a stable PAH hydrogel matrix used for DspB loading. The amount of DspB loaded increased linearly with the number of PAH layers in surface hydrogels. DspB was retained within these coatings in the pH range from 4 to 7.5. DspB-loaded coatings inhibited biofilm formation by two clinical strains of Staphylococcus epidermidis. Biofilm inhibition was ≥98% compared to mock-loaded coatings as determined by CFU enumeration. In addition, DspB-loaded coatings did not inhibit attachment or growth of cultured human osteoblast cells. We suggest that the use of DspB-loaded multilayer coatings presents a promising method for creating biocompatible surfaces with high antibiofilm efficiency, especially when combined with conventional antimicrobial treatment of dispersed bacteria.

Original languageEnglish
Pages (from-to)4708-4716
Number of pages9
JournalACS Applied Materials and Interfaces
Volume4
Issue number9
DOIs
Publication statusPublished - Sep 26 2012
Externally publishedYes

Fingerprint

Biofilms
Coatings
Polycyclic aromatic hydrocarbons
Hydrogels
Staphylococcus epidermidis
Hydrogel
Glutaral
Static Electricity
Osteoblasts
Extracellular Matrix
Polymers
Bacteria
Acids
Coulomb interactions
Polymer matrix
Enzymes
Growth
Multilayers
methacrylic acid

Keywords

  • biocompatibility
  • biofilm inhibition
  • cytotoxicity
  • dispersin B
  • layer-by-layer
  • Staphylococcus epidermidis

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this

Pavlukhina, S. V., Kaplan, J. B., Xu, L., Chang, W., Yu, X., Madhyastha, S., ... Sukhishvili, S. A. (2012). Noneluting enzymatic antibiofilm coatings. ACS Applied Materials and Interfaces, 4(9), 4708-4716. https://doi.org/10.1021/am3010847

Noneluting enzymatic antibiofilm coatings. / Pavlukhina, Svetlana V.; Kaplan, Jeffrey B.; Xu, Li; Chang, Wei; Yu, Xiaojun; Madhyastha, Srinivasa; Yakandawala, Nandadeva; Mentbayeva, Almagul; Khan, Babar; Sukhishvili, Svetlana A.

In: ACS Applied Materials and Interfaces, Vol. 4, No. 9, 26.09.2012, p. 4708-4716.

Research output: Contribution to journalArticle

Pavlukhina, SV, Kaplan, JB, Xu, L, Chang, W, Yu, X, Madhyastha, S, Yakandawala, N, Mentbayeva, A, Khan, B & Sukhishvili, SA 2012, 'Noneluting enzymatic antibiofilm coatings', ACS Applied Materials and Interfaces, vol. 4, no. 9, pp. 4708-4716. https://doi.org/10.1021/am3010847
Pavlukhina SV, Kaplan JB, Xu L, Chang W, Yu X, Madhyastha S et al. Noneluting enzymatic antibiofilm coatings. ACS Applied Materials and Interfaces. 2012 Sep 26;4(9):4708-4716. https://doi.org/10.1021/am3010847
Pavlukhina, Svetlana V. ; Kaplan, Jeffrey B. ; Xu, Li ; Chang, Wei ; Yu, Xiaojun ; Madhyastha, Srinivasa ; Yakandawala, Nandadeva ; Mentbayeva, Almagul ; Khan, Babar ; Sukhishvili, Svetlana A. / Noneluting enzymatic antibiofilm coatings. In: ACS Applied Materials and Interfaces. 2012 ; Vol. 4, No. 9. pp. 4708-4716.
@article{ead84d556bc0484bb52833954d12c6a4,
title = "Noneluting enzymatic antibiofilm coatings",
abstract = "We developed a highly efficient, biocompatible surface coating that disperses bacterial biofilms through enzymatic cleavage of the extracellular biofilm matrix. The coating was fabricated by binding the naturally existing enzyme dispersin B (DspB) to surface-attached polymer matrices constructed via a layer-by-layer (LbL) deposition technique. LbL matrices were assembled through electrostatic interactions of poly(allylamine hydrochloride) (PAH) and poly(methacrylic acid) (PMAA), followed by chemical cross-linking with glutaraldehyde and pH-triggered removal of PMAA, producing a stable PAH hydrogel matrix used for DspB loading. The amount of DspB loaded increased linearly with the number of PAH layers in surface hydrogels. DspB was retained within these coatings in the pH range from 4 to 7.5. DspB-loaded coatings inhibited biofilm formation by two clinical strains of Staphylococcus epidermidis. Biofilm inhibition was ≥98{\%} compared to mock-loaded coatings as determined by CFU enumeration. In addition, DspB-loaded coatings did not inhibit attachment or growth of cultured human osteoblast cells. We suggest that the use of DspB-loaded multilayer coatings presents a promising method for creating biocompatible surfaces with high antibiofilm efficiency, especially when combined with conventional antimicrobial treatment of dispersed bacteria.",
keywords = "biocompatibility, biofilm inhibition, cytotoxicity, dispersin B, layer-by-layer, Staphylococcus epidermidis",
author = "Pavlukhina, {Svetlana V.} and Kaplan, {Jeffrey B.} and Li Xu and Wei Chang and Xiaojun Yu and Srinivasa Madhyastha and Nandadeva Yakandawala and Almagul Mentbayeva and Babar Khan and Sukhishvili, {Svetlana A.}",
year = "2012",
month = "9",
day = "26",
doi = "10.1021/am3010847",
language = "English",
volume = "4",
pages = "4708--4716",
journal = "ACS applied materials & interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Noneluting enzymatic antibiofilm coatings

AU - Pavlukhina, Svetlana V.

AU - Kaplan, Jeffrey B.

AU - Xu, Li

AU - Chang, Wei

AU - Yu, Xiaojun

AU - Madhyastha, Srinivasa

AU - Yakandawala, Nandadeva

AU - Mentbayeva, Almagul

AU - Khan, Babar

AU - Sukhishvili, Svetlana A.

PY - 2012/9/26

Y1 - 2012/9/26

N2 - We developed a highly efficient, biocompatible surface coating that disperses bacterial biofilms through enzymatic cleavage of the extracellular biofilm matrix. The coating was fabricated by binding the naturally existing enzyme dispersin B (DspB) to surface-attached polymer matrices constructed via a layer-by-layer (LbL) deposition technique. LbL matrices were assembled through electrostatic interactions of poly(allylamine hydrochloride) (PAH) and poly(methacrylic acid) (PMAA), followed by chemical cross-linking with glutaraldehyde and pH-triggered removal of PMAA, producing a stable PAH hydrogel matrix used for DspB loading. The amount of DspB loaded increased linearly with the number of PAH layers in surface hydrogels. DspB was retained within these coatings in the pH range from 4 to 7.5. DspB-loaded coatings inhibited biofilm formation by two clinical strains of Staphylococcus epidermidis. Biofilm inhibition was ≥98% compared to mock-loaded coatings as determined by CFU enumeration. In addition, DspB-loaded coatings did not inhibit attachment or growth of cultured human osteoblast cells. We suggest that the use of DspB-loaded multilayer coatings presents a promising method for creating biocompatible surfaces with high antibiofilm efficiency, especially when combined with conventional antimicrobial treatment of dispersed bacteria.

AB - We developed a highly efficient, biocompatible surface coating that disperses bacterial biofilms through enzymatic cleavage of the extracellular biofilm matrix. The coating was fabricated by binding the naturally existing enzyme dispersin B (DspB) to surface-attached polymer matrices constructed via a layer-by-layer (LbL) deposition technique. LbL matrices were assembled through electrostatic interactions of poly(allylamine hydrochloride) (PAH) and poly(methacrylic acid) (PMAA), followed by chemical cross-linking with glutaraldehyde and pH-triggered removal of PMAA, producing a stable PAH hydrogel matrix used for DspB loading. The amount of DspB loaded increased linearly with the number of PAH layers in surface hydrogels. DspB was retained within these coatings in the pH range from 4 to 7.5. DspB-loaded coatings inhibited biofilm formation by two clinical strains of Staphylococcus epidermidis. Biofilm inhibition was ≥98% compared to mock-loaded coatings as determined by CFU enumeration. In addition, DspB-loaded coatings did not inhibit attachment or growth of cultured human osteoblast cells. We suggest that the use of DspB-loaded multilayer coatings presents a promising method for creating biocompatible surfaces with high antibiofilm efficiency, especially when combined with conventional antimicrobial treatment of dispersed bacteria.

KW - biocompatibility

KW - biofilm inhibition

KW - cytotoxicity

KW - dispersin B

KW - layer-by-layer

KW - Staphylococcus epidermidis

UR - http://www.scopus.com/inward/record.url?scp=84867484922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867484922&partnerID=8YFLogxK

U2 - 10.1021/am3010847

DO - 10.1021/am3010847

M3 - Article

VL - 4

SP - 4708

EP - 4716

JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

SN - 1944-8244

IS - 9

ER -