Novel myeloma-associated antigens revealed in the context of syngeneic hematopoietic stem cell transplantation

Melinda A. Biernacki, Yu Tzu Tai, Guang Lan Zhang, Anselmo Alonso, Wandi Zhang, Rao Prabhala, Li Zhang, Nikhil Munshi, Donna Neuberg, Robert J. Soiffer, Jerome Ritz, Edwin P. Alyea, Vladimir Brusic, Kenneth C. Anderson, Catherine J. Wu

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Targets of curative donor-derived graft-versus-myeloma (GVM) responses after allogeneic hematopoietic stem cell transplantation (HSCT) remain poorly defined, partly because immunity against minor histocompatibility Ags (mHAgs) complicates the elucidation of multiple myeloma (MM)-specific targets. We hypothesized that syngeneic HSCT would facilitate the identification of GVM-associated Ags because donor immune responses in this setting should exclusively target unique tumor Ags in the absence of donor-host genetic disparities. Therefore, in the present study, we investigated the development of tumor immunity in an HLA-A0201+ MM patient who achieved durable remission after myeloablative syngeneic HSCT. Using high-density protein microarrays to screen post-HSCT plasma, we identified 6 Ags that elicited high-titer (1:5000-1:10 000) Abs that correlated with clinical tumor regression. Two Ags (DAPK2 and PIM1) had enriched expression in primary MM tissues. Both elicited Ab responses in other MM patients after chemotherapy or HSCT (11 and 6 of 32 patients for DAPK2 and PIM1, respectively). The index patient also developed specific CD8+ T-cell responses to HLA-A2-restricted peptides derived from DAPK2 and PIM1. Peptide-specific T cells recognized HLA-A2+ MM-derived cell lines and primary MM tumor cells. Coordinated T- and B-cell immunity develops against MM-associatedAgs after syngeneic HSCT. DAPK1 and PIM1 are promising target Ags for MM-directed immunotherapy.

Original languageEnglish
Pages (from-to)3142-3150
Number of pages9
JournalBlood
Volume119
Issue number13
DOIs
Publication statusPublished - Mar 29 2012

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint Dive into the research topics of 'Novel myeloma-associated antigens revealed in the context of syngeneic hematopoietic stem cell transplantation'. Together they form a unique fingerprint.

Cite this