Nucleocapsid Protein of SARS-CoV-2 Upregulates RANTES Expression in A172 Glioblastoma Cells

Bakhytgul Gadilgereyeva; Zhanar Kunushpayeva; Mira Abdrakhmanova; Aizere Khassenova; Nail Minigulov; Timo Burster; Olena Filchakova

doi:10.3390/molecules30051066

Nucleocapsid Protein of SARS-CoV-2 Upregulates RANTES Expression in A172 Glioblastoma Cells

Bakhytgul Gadilgereyeva, Zhanar Kunushpayeva, Mira Abdrakhmanova, Aizere Khassenova, Nail Minigulov, Timo Burster, Olena Filchakova

School of Sciences and Humanities

Research output: Contribution to journal › Article › peer-review

Abstract

SARS-CoV-2, the pathogenic virus that induces COVID-19 disease, contains four structural proteins in its virion. The nucleocapsid (N) protein is one of the four structural proteins that play a crucial role in the assembly of viral RNA into ribonucleoprotein. In addition, the N protein contributes to viral pathogenesis. One of the functions attributed to the N protein is the triggering of cytokine release by lung epithelial cells, macrophages, and monocytes. This study addresses the cellular effects of the N protein of SARS-CoV-2 on cells of glial origin. We report the upregulation of the RANTES chemokine in A172 glioblastoma cells at both the mRNA and protein levels in response to exposure to SARS-CoV-2 nucleocapsid protein. The N protein did not have an effect on cell viability and cell migration.

Original language	English
Article number	1066
Journal	Molecules
Volume	30
Issue number	5
DOIs	https://doi.org/10.3390/molecules30051066
Publication status	Published - Mar 2025

Keywords

coronaviruses
glioblastoma
nucleocapsid protein
RANTES
SARS-CoV-2

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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10.3390/molecules30051066

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title = "Nucleocapsid Protein of SARS-CoV-2 Upregulates RANTES Expression in A172 Glioblastoma Cells",

abstract = "SARS-CoV-2, the pathogenic virus that induces COVID-19 disease, contains four structural proteins in its virion. The nucleocapsid (N) protein is one of the four structural proteins that play a crucial role in the assembly of viral RNA into ribonucleoprotein. In addition, the N protein contributes to viral pathogenesis. One of the functions attributed to the N protein is the triggering of cytokine release by lung epithelial cells, macrophages, and monocytes. This study addresses the cellular effects of the N protein of SARS-CoV-2 on cells of glial origin. We report the upregulation of the RANTES chemokine in A172 glioblastoma cells at both the mRNA and protein levels in response to exposure to SARS-CoV-2 nucleocapsid protein. The N protein did not have an effect on cell viability and cell migration.",

keywords = "coronaviruses, glioblastoma, nucleocapsid protein, RANTES, SARS-CoV-2",

author = "Bakhytgul Gadilgereyeva and Zhanar Kunushpayeva and Mira Abdrakhmanova and Aizere Khassenova and Nail Minigulov and Timo Burster and Olena Filchakova",

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year = "2025",

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doi = "10.3390/molecules30051066",

language = "English",

volume = "30",

journal = "Molecules",

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T1 - Nucleocapsid Protein of SARS-CoV-2 Upregulates RANTES Expression in A172 Glioblastoma Cells

AU - Gadilgereyeva, Bakhytgul

AU - Kunushpayeva, Zhanar

AU - Abdrakhmanova, Mira

AU - Khassenova, Aizere

AU - Minigulov, Nail

AU - Burster, Timo

AU - Filchakova, Olena

PY - 2025/3

Y1 - 2025/3

N2 - SARS-CoV-2, the pathogenic virus that induces COVID-19 disease, contains four structural proteins in its virion. The nucleocapsid (N) protein is one of the four structural proteins that play a crucial role in the assembly of viral RNA into ribonucleoprotein. In addition, the N protein contributes to viral pathogenesis. One of the functions attributed to the N protein is the triggering of cytokine release by lung epithelial cells, macrophages, and monocytes. This study addresses the cellular effects of the N protein of SARS-CoV-2 on cells of glial origin. We report the upregulation of the RANTES chemokine in A172 glioblastoma cells at both the mRNA and protein levels in response to exposure to SARS-CoV-2 nucleocapsid protein. The N protein did not have an effect on cell viability and cell migration.

AB - SARS-CoV-2, the pathogenic virus that induces COVID-19 disease, contains four structural proteins in its virion. The nucleocapsid (N) protein is one of the four structural proteins that play a crucial role in the assembly of viral RNA into ribonucleoprotein. In addition, the N protein contributes to viral pathogenesis. One of the functions attributed to the N protein is the triggering of cytokine release by lung epithelial cells, macrophages, and monocytes. This study addresses the cellular effects of the N protein of SARS-CoV-2 on cells of glial origin. We report the upregulation of the RANTES chemokine in A172 glioblastoma cells at both the mRNA and protein levels in response to exposure to SARS-CoV-2 nucleocapsid protein. The N protein did not have an effect on cell viability and cell migration.

KW - coronaviruses

KW - glioblastoma

KW - nucleocapsid protein

KW - RANTES

KW - SARS-CoV-2

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U2 - 10.3390/molecules30051066

DO - 10.3390/molecules30051066

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AN - SCOPUS:86000618299

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JF - Molecules

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M1 - 1066

ER -

Nucleocapsid Protein of SARS-CoV-2 Upregulates RANTES Expression in A172 Glioblastoma Cells

Abstract

Keywords

ASJC Scopus subject areas

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