Occludin binds to the SH3-hinge-GuK unit of zonula occludens protein 1: Potential mechanism of tight junction regulation

A. Schmidt, D. I. Utepbergenov, S. L. Mueller, M. Beyermann, J. Schneider-Mergener, G. Krause, I. E. Blasig

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The interaction between tight junction proteins occludin and zona occludens protein 1 (ZO-1) was clarified. The sequence cc1 within the hinge region of ZO-1, connecting its SH3 and GuK domains, was identified as a new association site for the occludin C-terminus, core binding area GLRSSKRNLRKSR (mouse ZO-1606-618). Occludin also bound to the sequence H2 within GuK, core area HKLRKNNH (ZO-1759-766). In occludin, the binding core was ELSRLDKELDDYREESEEY (mouse occludin455-473). Helicity of the sequences was suggested by circular dichroism. Because basic residues in ZO-1, acidic residues in occludin (underlined), coiled-coil helix-forming leucine heptad motifs (bold) in occludin and, probably, in cc1 were essential, we conclude that interactions were both helical and ionic. Moreover, the GuK domain bound other GuK molecules, suggesting oligomerization of ZO-1. Generally, the assumption is supported that the SH3-hinge-GuK region represents a functional and regulatory unit in ZO-1 forming a multiprotein tight junction complex with occludin.

Original languageEnglish
Pages (from-to)1354-1365
Number of pages12
JournalCellular and Molecular Life Sciences
Volume61
Issue number11
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

Fingerprint

Zonula Occludens-1 Protein
Occludin
Tight Junctions
Hinges
Tight Junction Proteins
Oligomerization
src Homology Domains
Circular Dichroism
Leucine
Association reactions
Molecules

Keywords

  • Adaptor protein
  • MAGuK protein family
  • Occludin
  • Protein interaction
  • Tight junction
  • ZO-1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Occludin binds to the SH3-hinge-GuK unit of zonula occludens protein 1 : Potential mechanism of tight junction regulation. / Schmidt, A.; Utepbergenov, D. I.; Mueller, S. L.; Beyermann, M.; Schneider-Mergener, J.; Krause, G.; Blasig, I. E.

In: Cellular and Molecular Life Sciences, Vol. 61, No. 11, 06.2004, p. 1354-1365.

Research output: Contribution to journalArticle

Schmidt, A. ; Utepbergenov, D. I. ; Mueller, S. L. ; Beyermann, M. ; Schneider-Mergener, J. ; Krause, G. ; Blasig, I. E. / Occludin binds to the SH3-hinge-GuK unit of zonula occludens protein 1 : Potential mechanism of tight junction regulation. In: Cellular and Molecular Life Sciences. 2004 ; Vol. 61, No. 11. pp. 1354-1365.
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