Abstract
Point mutations occurring at codon 201 of the gene coding for the α subunit of the stimulatory G protein impair intrinsic GTPase activity and lead to a constitutive activation of adenylate cyclase. We have examined thyroid follicular and papillary carcinomas and follicular adenomas and found samples that bear this mutation at codon 201 of the Gsα gene. Both mutation-positive and mutation-negative tissue samples were investigated for the level of Gsα expression relative to a pool of normal thyroid tissue, using immunoblotting against two (mid-region-specific and C-end-specific) antipeptide antibodies. Using 8000 g and 100 000 g membrane fractions of homogenized tissues we have demonstrated that the Gsα proteins in normal ad neoplastic thyroid tissues are represented by three isoforms: 43 kDa, 45 kDa and 52 kDa. We have quantified and compared the amount of Gsα protein and find it is overexpressed in mutation-bearing tissue samples.
| Original language | English |
|---|---|
| Pages (from-to) | 219-224 |
| Number of pages | 6 |
| Journal | Journal of Cancer Research and Clinical Oncology |
| Volume | 121 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1 1995 |
| Externally published | Yes |
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Keywords
- Expression
- Gsα gene
- Immunoblotting
- Point mutation
- Thyroid tumors
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Overexpression of Gsα subunit in thyroid tumors bearing a mutated Gsα gene. / Gorelov, Victor N.; Dumon, Kristoffel; Barteneva, Natalie S.; Palm, Dieter; Röher, Hans Dieter; Goretzki, Peter E.
In: Journal of Cancer Research and Clinical Oncology, Vol. 121, No. 4, 01.04.1995, p. 219-224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of Gsα subunit in thyroid tumors bearing a mutated Gsα gene
AU - Gorelov, Victor N.
AU - Dumon, Kristoffel
AU - Barteneva, Natalie S.
AU - Palm, Dieter
AU - Röher, Hans Dieter
AU - Goretzki, Peter E.
PY - 1995/4/1
Y1 - 1995/4/1
N2 - Point mutations occurring at codon 201 of the gene coding for the α subunit of the stimulatory G protein impair intrinsic GTPase activity and lead to a constitutive activation of adenylate cyclase. We have examined thyroid follicular and papillary carcinomas and follicular adenomas and found samples that bear this mutation at codon 201 of the Gsα gene. Both mutation-positive and mutation-negative tissue samples were investigated for the level of Gsα expression relative to a pool of normal thyroid tissue, using immunoblotting against two (mid-region-specific and C-end-specific) antipeptide antibodies. Using 8000 g and 100 000 g membrane fractions of homogenized tissues we have demonstrated that the Gsα proteins in normal ad neoplastic thyroid tissues are represented by three isoforms: 43 kDa, 45 kDa and 52 kDa. We have quantified and compared the amount of Gsα protein and find it is overexpressed in mutation-bearing tissue samples.
AB - Point mutations occurring at codon 201 of the gene coding for the α subunit of the stimulatory G protein impair intrinsic GTPase activity and lead to a constitutive activation of adenylate cyclase. We have examined thyroid follicular and papillary carcinomas and follicular adenomas and found samples that bear this mutation at codon 201 of the Gsα gene. Both mutation-positive and mutation-negative tissue samples were investigated for the level of Gsα expression relative to a pool of normal thyroid tissue, using immunoblotting against two (mid-region-specific and C-end-specific) antipeptide antibodies. Using 8000 g and 100 000 g membrane fractions of homogenized tissues we have demonstrated that the Gsα proteins in normal ad neoplastic thyroid tissues are represented by three isoforms: 43 kDa, 45 kDa and 52 kDa. We have quantified and compared the amount of Gsα protein and find it is overexpressed in mutation-bearing tissue samples.
KW - Expression
KW - Gsα gene
KW - Immunoblotting
KW - Point mutation
KW - Thyroid tumors
UR - http://www.scopus.com/inward/record.url?scp=0028988226&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028988226&partnerID=8YFLogxK
U2 - 10.1007/BF01366965
DO - 10.1007/BF01366965
M3 - Article
VL - 121
SP - 219
EP - 224
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
SN - 0171-5216
IS - 4
ER -