Abstract
Impact of global warming is increasing thus exacerbating environmental stresses
which affect crop yields. Studying senescence and the genes that are related to stress
is important in crops in these changing climatic conditions. Previously, the
senescence-associated receptor-like kinase (SARK) was shown as a positive
regulator during the natural senescence in A. thaliana, G.max, although in common
bean (Phaseolus vulgaris L.) it did not coordinate senescence in noodle. Here we
designed the overexpression of AtSARK (OESARK) tagged with His under strong
promoter (35S), which was later transformed by dipping method into Arabidopsis (Col)
ecotype. OESARK lines were isolated by BASTA and verified by PCR and sequencing analysis. Later, the mRNA and protein of SARK were detected confirming the overexpression of SARK in new generated mutant lines (6 lines). Interestingly, OESARK lines (in the tested 6 lines) didn't show the enhancement of senescence symptoms compared to WT (Col). Additionally, the microarray data analysis (GENEVESTIGATOR; https://www.genevestigator.ethz.ch) of AtSARK showed that AtSARK changed the expression pattern under biotic and abiotic stresses such as cold, heat stresses and L. huidobrensis, P. parasitica, P. syringae pv maculicola. This is a promising study on the impact of these stresses on the new generated mutant line in the future.
which affect crop yields. Studying senescence and the genes that are related to stress
is important in crops in these changing climatic conditions. Previously, the
senescence-associated receptor-like kinase (SARK) was shown as a positive
regulator during the natural senescence in A. thaliana, G.max, although in common
bean (Phaseolus vulgaris L.) it did not coordinate senescence in noodle. Here we
designed the overexpression of AtSARK (OESARK) tagged with His under strong
promoter (35S), which was later transformed by dipping method into Arabidopsis (Col)
ecotype. OESARK lines were isolated by BASTA and verified by PCR and sequencing analysis. Later, the mRNA and protein of SARK were detected confirming the overexpression of SARK in new generated mutant lines (6 lines). Interestingly, OESARK lines (in the tested 6 lines) didn't show the enhancement of senescence symptoms compared to WT (Col). Additionally, the microarray data analysis (GENEVESTIGATOR; https://www.genevestigator.ethz.ch) of AtSARK showed that AtSARK changed the expression pattern under biotic and abiotic stresses such as cold, heat stresses and L. huidobrensis, P. parasitica, P. syringae pv maculicola. This is a promising study on the impact of these stresses on the new generated mutant line in the future.
Original language | English |
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Title of host publication | 32nd International Conference on Arabidopsis Research |
Publication status | Published - Jun 22 2022 |