Abstract
Macroporous scaffolds composed of chitosan and using oxidized dextran as a crosslinker are produced through cryogelation. Introducing gelatin as a third component into the structure results in the formation of mesopores in the pore walls, which are not seen if gelatin is excluded. The mesoporous structure is explained by the formation of polyelectrolyte complexes between chitosan and gelatin before crosslinking takes place. The scaffolds exhibit highly elastic properties withstanding compressions up to 60%. The in vitro biocompatibility of the cryogels is evaluated using fibroblasts from a mouse cell line (L929) and it is seen that the cells adhere and proliferate on the scaffolds. The mesoporous structure seems to have a positive effect on proliferation. The formation of PECs between chitosan and gelatin under subzero conditions in a one-step preparation using oxidized dextran as a crosslinker is presented. These scaffolds exhibit a heterogeneous structure with macropores and mesoporous pore walls. Fibroblast cultures show that the PEC-based materials have better cell growth compared to scaffolds with no PEC.
| Original language | English |
|---|---|
| Pages (from-to) | 1090-1099 |
| Number of pages | 10 |
| Journal | Macromolecular Bioscience |
| Volume | 12 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 2012 |
| Externally published | Yes |
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Keywords
- Cryogels
- Oxidized dextran
- Polyelectrolyte complexes
- Tissue engineering
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomaterials
- Polymers and Plastics
- Materials Chemistry
Cite this
Oxidized Dextran as Crosslinker for Chitosan Cryogel Scaffolds and Formation of Polyelectrolyte Complexes between Chitosan and Gelatin. / Berillo, Dmitriy; Elowsson, Linda; Kirsebom, Harald.
In: Macromolecular Bioscience, Vol. 12, No. 8, 08.2012, p. 1090-1099.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxidized Dextran as Crosslinker for Chitosan Cryogel Scaffolds and Formation of Polyelectrolyte Complexes between Chitosan and Gelatin
AU - Berillo, Dmitriy
AU - Elowsson, Linda
AU - Kirsebom, Harald
PY - 2012/8
Y1 - 2012/8
N2 - Macroporous scaffolds composed of chitosan and using oxidized dextran as a crosslinker are produced through cryogelation. Introducing gelatin as a third component into the structure results in the formation of mesopores in the pore walls, which are not seen if gelatin is excluded. The mesoporous structure is explained by the formation of polyelectrolyte complexes between chitosan and gelatin before crosslinking takes place. The scaffolds exhibit highly elastic properties withstanding compressions up to 60%. The in vitro biocompatibility of the cryogels is evaluated using fibroblasts from a mouse cell line (L929) and it is seen that the cells adhere and proliferate on the scaffolds. The mesoporous structure seems to have a positive effect on proliferation. The formation of PECs between chitosan and gelatin under subzero conditions in a one-step preparation using oxidized dextran as a crosslinker is presented. These scaffolds exhibit a heterogeneous structure with macropores and mesoporous pore walls. Fibroblast cultures show that the PEC-based materials have better cell growth compared to scaffolds with no PEC.
AB - Macroporous scaffolds composed of chitosan and using oxidized dextran as a crosslinker are produced through cryogelation. Introducing gelatin as a third component into the structure results in the formation of mesopores in the pore walls, which are not seen if gelatin is excluded. The mesoporous structure is explained by the formation of polyelectrolyte complexes between chitosan and gelatin before crosslinking takes place. The scaffolds exhibit highly elastic properties withstanding compressions up to 60%. The in vitro biocompatibility of the cryogels is evaluated using fibroblasts from a mouse cell line (L929) and it is seen that the cells adhere and proliferate on the scaffolds. The mesoporous structure seems to have a positive effect on proliferation. The formation of PECs between chitosan and gelatin under subzero conditions in a one-step preparation using oxidized dextran as a crosslinker is presented. These scaffolds exhibit a heterogeneous structure with macropores and mesoporous pore walls. Fibroblast cultures show that the PEC-based materials have better cell growth compared to scaffolds with no PEC.
KW - Cryogels
KW - Oxidized dextran
KW - Polyelectrolyte complexes
KW - Tissue engineering
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UR - http://www.scopus.com/inward/citedby.url?scp=84864797942&partnerID=8YFLogxK
U2 - 10.1002/mabi.201200023
DO - 10.1002/mabi.201200023
M3 - Article
VL - 12
SP - 1090
EP - 1099
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
SN - 1616-5187
IS - 8
ER -