Oxygen transfer to cell culture by membrane diffusers

A. Bottino, G. Capannelli, A. Comite, R. Di Felice

Research output: Contribution to journalArticle

Abstract

The oxygen supply to cell cultures of Saccharomices cerevisiae by porous membranes has been investigated. Two diffusers with different surface areas were prepared by end sealed capillary polypropylene membranes with a nominal pore diameter of 0.2 μm. The diffusers were immersed in the cell culture kept under constant agitation. Air was fed in the membrane lumen and diffused into the cell culture without any visible large bubble formation. Bath and fed-batch cultivations were performed and the variation of cell, glucose and oxygen concentration, during the cultivation, was investigated. The results obtained by membrane diffuser were compared with those of a traditional gas sparger. The advantages of membrane diffuser over the sparger are discussed.

Original languageEnglish
JournalChemical and Biochemical Engineering Quarterly
Volume19
Issue number3
Publication statusPublished - Sep 2005
Externally publishedYes

Fingerprint

Cell culture
Cell Culture Techniques
Cell Membrane
Oxygen
Membranes
Oxygen supply
Bubble formation
Polypropylenes
Baths
Glucose
Gases
Air

Keywords

  • Capillary membrane
  • Cell culture
  • Membrane diffuser
  • Oxygen transfer
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Biotechnology

Cite this

Bottino, A., Capannelli, G., Comite, A., & Di Felice, R. (2005). Oxygen transfer to cell culture by membrane diffusers. Chemical and Biochemical Engineering Quarterly, 19(3).

Oxygen transfer to cell culture by membrane diffusers. / Bottino, A.; Capannelli, G.; Comite, A.; Di Felice, R.

In: Chemical and Biochemical Engineering Quarterly, Vol. 19, No. 3, 09.2005.

Research output: Contribution to journalArticle

Bottino, A, Capannelli, G, Comite, A & Di Felice, R 2005, 'Oxygen transfer to cell culture by membrane diffusers', Chemical and Biochemical Engineering Quarterly, vol. 19, no. 3.
Bottino A, Capannelli G, Comite A, Di Felice R. Oxygen transfer to cell culture by membrane diffusers. Chemical and Biochemical Engineering Quarterly. 2005 Sep;19(3).
Bottino, A. ; Capannelli, G. ; Comite, A. ; Di Felice, R. / Oxygen transfer to cell culture by membrane diffusers. In: Chemical and Biochemical Engineering Quarterly. 2005 ; Vol. 19, No. 3.
@article{56b44a9112f24ce98b8115dd2fcfa874,
title = "Oxygen transfer to cell culture by membrane diffusers",
abstract = "The oxygen supply to cell cultures of Saccharomices cerevisiae by porous membranes has been investigated. Two diffusers with different surface areas were prepared by end sealed capillary polypropylene membranes with a nominal pore diameter of 0.2 μm. The diffusers were immersed in the cell culture kept under constant agitation. Air was fed in the membrane lumen and diffused into the cell culture without any visible large bubble formation. Bath and fed-batch cultivations were performed and the variation of cell, glucose and oxygen concentration, during the cultivation, was investigated. The results obtained by membrane diffuser were compared with those of a traditional gas sparger. The advantages of membrane diffuser over the sparger are discussed.",
keywords = "Capillary membrane, Cell culture, Membrane diffuser, Oxygen transfer, Saccharomyces cerevisiae",
author = "A. Bottino and G. Capannelli and A. Comite and {Di Felice}, R.",
year = "2005",
month = "9",
language = "English",
volume = "19",
journal = "Chemical and Biochemical Engineering Quarterly",
issn = "0352-9568",
publisher = "Assoc. of Chemists and Chemical Engineers of Croatia",
number = "3",

}

TY - JOUR

T1 - Oxygen transfer to cell culture by membrane diffusers

AU - Bottino, A.

AU - Capannelli, G.

AU - Comite, A.

AU - Di Felice, R.

PY - 2005/9

Y1 - 2005/9

N2 - The oxygen supply to cell cultures of Saccharomices cerevisiae by porous membranes has been investigated. Two diffusers with different surface areas were prepared by end sealed capillary polypropylene membranes with a nominal pore diameter of 0.2 μm. The diffusers were immersed in the cell culture kept under constant agitation. Air was fed in the membrane lumen and diffused into the cell culture without any visible large bubble formation. Bath and fed-batch cultivations were performed and the variation of cell, glucose and oxygen concentration, during the cultivation, was investigated. The results obtained by membrane diffuser were compared with those of a traditional gas sparger. The advantages of membrane diffuser over the sparger are discussed.

AB - The oxygen supply to cell cultures of Saccharomices cerevisiae by porous membranes has been investigated. Two diffusers with different surface areas were prepared by end sealed capillary polypropylene membranes with a nominal pore diameter of 0.2 μm. The diffusers were immersed in the cell culture kept under constant agitation. Air was fed in the membrane lumen and diffused into the cell culture without any visible large bubble formation. Bath and fed-batch cultivations were performed and the variation of cell, glucose and oxygen concentration, during the cultivation, was investigated. The results obtained by membrane diffuser were compared with those of a traditional gas sparger. The advantages of membrane diffuser over the sparger are discussed.

KW - Capillary membrane

KW - Cell culture

KW - Membrane diffuser

KW - Oxygen transfer

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=26244464543&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26244464543&partnerID=8YFLogxK

M3 - Article

VL - 19

JO - Chemical and Biochemical Engineering Quarterly

JF - Chemical and Biochemical Engineering Quarterly

SN - 0352-9568

IS - 3

ER -