Phosphorylation of β-Catenin and epidermal growth factor receptor by intestinal trefoil factor

Dan Liu, Iman El-Hariry, Anastasios J. Karayiannakis, Jonathan Wilding, Rebecca Chinery, Witold Kmiot, Pierre D. McCrea, William J. Gullick, Massimo Pignatelli

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117 Citations (Scopus)

Abstract

Intestinal trefoil factor (TFF3) is a member of the trefoil family of peptides, which are constitutively expressed in the gastrointestinal tract. TFF3 has been shown to promote migration of intestinal epithelial cells in vitro and to enhance epithelial restitution in vivo. In the present study, we show that the stimulatory effect of TFF3 on the migration of HT29 colonic carcinoma cells requires the perturbation of E-cadherin function, a calcium- dependent cell-cell adhesion molecule in epithelia. A rapid (<1 minute) end specific tyrosine phosphoryletion of β-catenin and epidermal growth factor receptor was detected in cells treated with recombinant rat TFF3. No phosphorylation of E-cadherin or α-catenin was detected. Tyrosine phosphorylation of β-catenin was associated with reduced membranous E- cadherin expression, perturbation of intercellular adhesion, and promotion of cell motility. These results suggest that TFF3 enhances cell migration through modulation of E-cadherin/catenin complex function. Tyrosine phosphorylation of β-catenin and epidermal growth factor receptor seems to be involved in this process.

Original languageEnglish
Pages (from-to)557-563
Number of pages7
JournalLaboratory Investigation
Volume77
Issue number6
Publication statusPublished - Dec 1 1997

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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    Liu, D., El-Hariry, I., Karayiannakis, A. J., Wilding, J., Chinery, R., Kmiot, W., McCrea, P. D., Gullick, W. J., & Pignatelli, M. (1997). Phosphorylation of β-Catenin and epidermal growth factor receptor by intestinal trefoil factor. Laboratory Investigation, 77(6), 557-563.