Photosynthetic electron transport in the cyanobacterium synechocystis sp. PCC 6803

High-field W-band and X-band EPR study of electron flow through photosystem i

A. Savitsky, B. V. Trubitsin, K. Möbius, A. Yu Semenov, A. N. Tikhonov

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this work, by using the respective advantages of W- and X-band electron paramagnetic resonance (EPR) spectroscopy techniques to investigate electron transport processes, we have studied the light-induced redox transients of the primary electron donor P700 and the secondary acceptor A1 in photosystem (PS) I complexes of intact cyanobacterial cells Synechocystis sp. PCC 6803. We found that the kinetic behavior of the cation radical P 700 ̇+ generated by illumination with continuous light, and the EPR intensity of the radical pair P700 ̇+A1 ̇- generated upon laser pulse illumination strongly depend on the illumination prehistory (either the sample was frozen in the dark or during illumination). Both these processes were sensitive to the presence of electron transport inhibitors which block electron flow between the two photosystems. In line with our X-band EPR data on the kinetics of light-induced redox transients of P700, our high-field W-band EPR study of the radical-pair state P700 ̇+A1 ̇- shows that photosynthetic electron flow through the PS I reaction center is controlled both on the donor and on the acceptor side of PS I.

Original languageEnglish
Pages (from-to)221-236
Number of pages16
JournalApplied Magnetic Resonance
Volume31
Issue number1-2
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

superhigh frequencies
Photosystem I Protein Complex
Paramagnetic resonance
electron paramagnetic resonance
Lighting
illumination
Electrons
electrons
Kinetics
kinetics
Cations
Laser pulses
inhibitors
Spectroscopy
Cyanobacteria
Electron Transport
cations
pulses
cells
spectroscopy

ASJC Scopus subject areas

  • Spectroscopy
  • Atomic and Molecular Physics, and Optics

Cite this

Photosynthetic electron transport in the cyanobacterium synechocystis sp. PCC 6803 : High-field W-band and X-band EPR study of electron flow through photosystem i. / Savitsky, A.; Trubitsin, B. V.; Möbius, K.; Semenov, A. Yu; Tikhonov, A. N.

In: Applied Magnetic Resonance, Vol. 31, No. 1-2, 2007, p. 221-236.

Research output: Contribution to journalArticle

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