Picomolar detection of thrombin with fiber-optic ball resonator sensor using optical backscatter reflectometry

Takhmina Ayupova; Madina Shaimerdenova; Marzhan Sypabekova; Luca Vangelista; Daniele Tosi

doi:10.1016/j.ijleo.2021.166969

Picomolar detection of thrombin with fiber-optic ball resonator sensor using optical backscatter reflectometry

Takhmina Ayupova, Madina Shaimerdenova, Marzhan Sypabekova, Luca Vangelista, Daniele Tosi

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A ball resonator, positioned on the tip of an optical fiber, has been developed as a biosensor for the prototypic detection of thrombin. The device was fabricated with a fast and repeatable CO₂ laser splicing method, followed by gold-sputtering and functionalization for the measurement of various protein concentrations. The ball resonator acts as a weak interferometer with a return loss below − 50 dB, and it is interrogated with an optical backscatter reflectometer measuring the reflection spectrum. We report here a sample presenting high sensitivity (1273.74 nm/RIU, RIU = refractive index units), which allows protein detection in the range 0.4–100 pM, with a limit of detection of 1.56 pM in logarithmic response.

Original language	English
Article number	166969
Journal	Optik
Volume	241
DOIs	https://doi.org/10.1016/j.ijleo.2021.166969
Publication status	Published - Sept 2021

Keywords

Aptamer
Biosensor
Optical backscatter reflectometry
Optical ball resonator
Optical fiber biosensor
Thrombin

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering

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10.1016/j.ijleo.2021.166969

Cite this

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title = "Picomolar detection of thrombin with fiber-optic ball resonator sensor using optical backscatter reflectometry",

abstract = "A ball resonator, positioned on the tip of an optical fiber, has been developed as a biosensor for the prototypic detection of thrombin. The device was fabricated with a fast and repeatable CO2 laser splicing method, followed by gold-sputtering and functionalization for the measurement of various protein concentrations. The ball resonator acts as a weak interferometer with a return loss below − 50 dB, and it is interrogated with an optical backscatter reflectometer measuring the reflection spectrum. We report here a sample presenting high sensitivity (1273.74 nm/RIU, RIU = refractive index units), which allows protein detection in the range 0.4–100 pM, with a limit of detection of 1.56 pM in logarithmic response.",

keywords = "Aptamer, Biosensor, Optical backscatter reflectometry, Optical ball resonator, Optical fiber biosensor, Thrombin",

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AU - Ayupova, Takhmina

AU - Shaimerdenova, Madina

AU - Sypabekova, Marzhan

AU - Vangelista, Luca

AU - Tosi, Daniele

PY - 2021/9

Y1 - 2021/9

N2 - A ball resonator, positioned on the tip of an optical fiber, has been developed as a biosensor for the prototypic detection of thrombin. The device was fabricated with a fast and repeatable CO2 laser splicing method, followed by gold-sputtering and functionalization for the measurement of various protein concentrations. The ball resonator acts as a weak interferometer with a return loss below − 50 dB, and it is interrogated with an optical backscatter reflectometer measuring the reflection spectrum. We report here a sample presenting high sensitivity (1273.74 nm/RIU, RIU = refractive index units), which allows protein detection in the range 0.4–100 pM, with a limit of detection of 1.56 pM in logarithmic response.

AB - A ball resonator, positioned on the tip of an optical fiber, has been developed as a biosensor for the prototypic detection of thrombin. The device was fabricated with a fast and repeatable CO2 laser splicing method, followed by gold-sputtering and functionalization for the measurement of various protein concentrations. The ball resonator acts as a weak interferometer with a return loss below − 50 dB, and it is interrogated with an optical backscatter reflectometer measuring the reflection spectrum. We report here a sample presenting high sensitivity (1273.74 nm/RIU, RIU = refractive index units), which allows protein detection in the range 0.4–100 pM, with a limit of detection of 1.56 pM in logarithmic response.

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KW - Biosensor

KW - Optical backscatter reflectometry

KW - Optical ball resonator

KW - Optical fiber biosensor

KW - Thrombin

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Picomolar detection of thrombin with fiber-optic ball resonator sensor using optical backscatter reflectometry

Abstract

Keywords

ASJC Scopus subject areas

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