Plant extract of limonium gmelinii attenuates oxidative responses in neurons, astrocytes, and cerebral endothelial cells in vitro and improves motor functions of rats after middle cerebral artery occlusion

Tulendy Nurkenov, Andrey Tsoy, Farkhad Olzhayev, Elvira Abzhanova, Anel Turgambayeva, Aizhan Zhussupova, Bharathi Avula, Samir Ross, Aigerim Aituarova, Dariya Kassymova, Galiya Zhusupova, Tamara Shalakhmetova, Tursonjan Tokay, James C.M. Lee, Sholpan Askarova

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

There are numerous publications demonstrating that plant polyphenols can reduce oxi-dative stress and inflammatory processes in the brain. In the present study we have investigated the neuroprotective effect of plant extract isolated from the roots of L. gmelinii since it contains a rich source of polyphenols and other biologically active compounds. We have applied an oxidative and inflammatory model induced by NMDA, H2O2, and TNF‐α in human primary neurons and astrocytes, and mouse cerebral endothelial cell (CECs) line in vitro. The levels of ROS generation, NADPH oxidase activation, P‐selectin expression, and activity of ERK1/2 were evaluated by quan-titative immunofluorescence analysis, confocal microscopy, and MAPK assay. In vivo, sensorimotor functions in rats with middle cerebral artery occlusion (MCAO) were assessed. In neurons NMDA induced overproduction of ROS, in astrocytes TNF‐α initiated ROS generation, NADPH oxidase activation, and phosphorylation of ERK1/2. In CECs, the exposure by TNF‐α induced oxidative stress and triggered the accumulation of P‐selectin on the surface of the cells. In turn, pre‐treatment of the cells with the extract of L. gmelinii suppressed oxidative stress in all cell types and pro‐inflam-matory responses in astrocytes and CECs. In vivo, the treatment with L. gmelinii extract improved motor activity in rats with MCAO.

Original languageEnglish
Article number1814
JournalAntioxidants
Volume10
Issue number11
DOIs
Publication statusPublished - Nov 2021

Keywords

  • Antioxidants
  • Astrocytes
  • CECs
  • L. gmelinii
  • Neurons
  • Plant polyphenols

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint

Dive into the research topics of 'Plant extract of limonium gmelinii attenuates oxidative responses in neurons, astrocytes, and cerebral endothelial cells in vitro and improves motor functions of rats after middle cerebral artery occlusion'. Together they form a unique fingerprint.

Cite this