TY - JOUR
T1 - Plant extract of limonium gmelinii attenuates oxidative responses in neurons, astrocytes, and cerebral endothelial cells in vitro and improves motor functions of rats after middle cerebral artery occlusion
AU - Nurkenov, Tulendy
AU - Tsoy, Andrey
AU - Olzhayev, Farkhad
AU - Abzhanova, Elvira
AU - Turgambayeva, Anel
AU - Zhussupova, Aizhan
AU - Avula, Bharathi
AU - Ross, Samir
AU - Aituarova, Aigerim
AU - Kassymova, Dariya
AU - Zhusupova, Galiya
AU - Shalakhmetova, Tamara
AU - Tokay, Tursonjan
AU - Lee, James C.M.
AU - Askarova, Sholpan
N1 - Publisher Copyright:
© 2021 by the authors. Li-censee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - There are numerous publications demonstrating that plant polyphenols can reduce oxi-dative stress and inflammatory processes in the brain. In the present study we have investigated the neuroprotective effect of plant extract isolated from the roots of L. gmelinii since it contains a rich source of polyphenols and other biologically active compounds. We have applied an oxidative and inflammatory model induced by NMDA, H2O2, and TNF‐α in human primary neurons and astrocytes, and mouse cerebral endothelial cell (CECs) line in vitro. The levels of ROS generation, NADPH oxidase activation, P‐selectin expression, and activity of ERK1/2 were evaluated by quan-titative immunofluorescence analysis, confocal microscopy, and MAPK assay. In vivo, sensorimotor functions in rats with middle cerebral artery occlusion (MCAO) were assessed. In neurons NMDA induced overproduction of ROS, in astrocytes TNF‐α initiated ROS generation, NADPH oxidase activation, and phosphorylation of ERK1/2. In CECs, the exposure by TNF‐α induced oxidative stress and triggered the accumulation of P‐selectin on the surface of the cells. In turn, pre‐treatment of the cells with the extract of L. gmelinii suppressed oxidative stress in all cell types and pro‐inflam-matory responses in astrocytes and CECs. In vivo, the treatment with L. gmelinii extract improved motor activity in rats with MCAO.
AB - There are numerous publications demonstrating that plant polyphenols can reduce oxi-dative stress and inflammatory processes in the brain. In the present study we have investigated the neuroprotective effect of plant extract isolated from the roots of L. gmelinii since it contains a rich source of polyphenols and other biologically active compounds. We have applied an oxidative and inflammatory model induced by NMDA, H2O2, and TNF‐α in human primary neurons and astrocytes, and mouse cerebral endothelial cell (CECs) line in vitro. The levels of ROS generation, NADPH oxidase activation, P‐selectin expression, and activity of ERK1/2 were evaluated by quan-titative immunofluorescence analysis, confocal microscopy, and MAPK assay. In vivo, sensorimotor functions in rats with middle cerebral artery occlusion (MCAO) were assessed. In neurons NMDA induced overproduction of ROS, in astrocytes TNF‐α initiated ROS generation, NADPH oxidase activation, and phosphorylation of ERK1/2. In CECs, the exposure by TNF‐α induced oxidative stress and triggered the accumulation of P‐selectin on the surface of the cells. In turn, pre‐treatment of the cells with the extract of L. gmelinii suppressed oxidative stress in all cell types and pro‐inflam-matory responses in astrocytes and CECs. In vivo, the treatment with L. gmelinii extract improved motor activity in rats with MCAO.
KW - Antioxidants
KW - Astrocytes
KW - CECs
KW - L. gmelinii
KW - Neurons
KW - Plant polyphenols
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U2 - 10.3390/antiox10111814
DO - 10.3390/antiox10111814
M3 - Article
AN - SCOPUS:85118940547
SN - 2076-3921
VL - 10
JO - Antioxidants
JF - Antioxidants
IS - 11
M1 - 1814
ER -