Plasticity of HP1 proteins in mammalian cells

George K Dialynas, Stefan Terjung, Jeremy P Brown, Rebecca L Aucott, Bettina Baron-Luhr, Prim B Singh, Spyros D Georgatos

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We have compared the distribution of endogenous heterochromatin protein 1 (HP1) proteins (alpha, beta and gamma) in different epithelial lines, pluripotent stem cells and embryonic fibroblasts. In parallel, we have interrogated assembly and dynamics of newly expressed HP1-GFP proteins in cells lacking both HP1alpha and HP1beta alleles, blocked at the G1-S boundary, or cultured in the presence of HDAC and HAT inhibitors. The results reveal a range of cell type and differentiation state-specific patterns that do not correlate with 'fast' or 'slow' subunit exchange in heterochromatin. Furthermore, our observations show that targeting of HP1gamma to heterochromatic sites depends on HP1alpha and H1beta and that, on an architectural level, HP1alpha is the most polymorphic variant of the HP1 family. These data provide evidence for HP1 plasticity under shifting microenvironmental conditions and offer a new conceptual framework for understanding chromatin dynamics at the molecular level.

Original languageEnglish
Pages (from-to)3415-24
Number of pages10
JournalJournal of Cell Science
Volume120
Issue numberPt 19
DOIs
Publication statusPublished - Oct 1 2007

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Proteins
Pluripotent Stem Cells
Histone Deacetylase Inhibitors
Heterochromatin
Molecular Dynamics Simulation
Chromatin
Cell Differentiation
Fibroblasts
Alleles
heterochromatin-specific nonhistone chromosomal protein HP-1

Keywords

  • Animals
  • Cell Line
  • Chromosomal Proteins, Non-Histone
  • Epithelial Cells
  • Fibroblasts
  • Heterochromatin
  • Humans
  • Mice
  • Pluripotent Stem Cells
  • Protein Isoforms
  • Recombinant Fusion Proteins
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Dialynas, G. K., Terjung, S., Brown, J. P., Aucott, R. L., Baron-Luhr, B., Singh, P. B., & Georgatos, S. D. (2007). Plasticity of HP1 proteins in mammalian cells. Journal of Cell Science, 120(Pt 19), 3415-24. https://doi.org/10.1242/jcs.012914

Plasticity of HP1 proteins in mammalian cells. / Dialynas, George K; Terjung, Stefan; Brown, Jeremy P; Aucott, Rebecca L; Baron-Luhr, Bettina; Singh, Prim B; Georgatos, Spyros D.

In: Journal of Cell Science, Vol. 120, No. Pt 19, 01.10.2007, p. 3415-24.

Research output: Contribution to journalArticle

Dialynas, GK, Terjung, S, Brown, JP, Aucott, RL, Baron-Luhr, B, Singh, PB & Georgatos, SD 2007, 'Plasticity of HP1 proteins in mammalian cells', Journal of Cell Science, vol. 120, no. Pt 19, pp. 3415-24. https://doi.org/10.1242/jcs.012914
Dialynas GK, Terjung S, Brown JP, Aucott RL, Baron-Luhr B, Singh PB et al. Plasticity of HP1 proteins in mammalian cells. Journal of Cell Science. 2007 Oct 1;120(Pt 19):3415-24. https://doi.org/10.1242/jcs.012914
Dialynas, George K ; Terjung, Stefan ; Brown, Jeremy P ; Aucott, Rebecca L ; Baron-Luhr, Bettina ; Singh, Prim B ; Georgatos, Spyros D. / Plasticity of HP1 proteins in mammalian cells. In: Journal of Cell Science. 2007 ; Vol. 120, No. Pt 19. pp. 3415-24.
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