Platelets mediate protective neuroinflammation and promote neuronal plasticity at the site of neuronal injury

Marina Dukhinova, Inna Kuznetsova, Ekaterina Kopeikina, Ekaterina Veniaminova, Amanda W.Y. Yung, Tatyana Veremeyko, Kseniia Levchuk, Natalie Barteneva, Kenny Kam Wing-Ho, Wing Ho Yung, Julia Y.H. Liu, John Rudd, Sonata S.Y. Yau, Daniel C. Anthony, Tatyana Strekalova, Eugene D. Ponomarev

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

It is generally accepted that inflammation within the CNS contributes to neurodegeneration after traumatic brain injury (TBI), but it is not clear how inflammation is initiated in the absence of infection and whether this neuroinflammation is predominantly beneficial or detrimental. We have previously found that brain-enriched glycosphingolipids within neuronal lipid rafts (NLR) induced platelet degranulation and secretion of neurotransmitters and pro-inflammatory factors. In the present study, we compared TBI-induced inflammation and neurodegeneration in wild-type vs. St3gal5 deficient (ST3−/−) mice that lack major CNS-specific glycosphingolipids. After TBI, microglial activation and CNS macrophage infiltration were substantially reduced in ST3−/− animals. However, ST3−/− mice had a larger area of CNS damage with marked neuronal/axonal loss. The interaction of platelets with NLR stimulated neurite growth, increased the number of PSD95-positive dendritic spines, and intensified neuronal activity. Adoptive transfer and blocking experiments provide further that platelet-derived serotonin and platelet activating factor plays a key role in the regulation of sterile neuroinflammation, hemorrhage and neuronal plasticity after TBI.

Original languageEnglish
JournalBrain, Behavior, and Immunity
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Neuronal Plasticity
Blood Platelets
Wounds and Injuries
Inflammation
Lipids
Glycosphingolipids
Dendritic Spines
Macrophage Activation
Adoptive Transfer
Platelet Activating Factor
Neurites
Neurotransmitter Agents
Serotonin
Hemorrhage
Traumatic Brain Injury
Brain
Growth
Infection

Keywords

  • CNS repair
  • Dendritic spines
  • Glycobiology
  • Neuroinflammation
  • Neuronal plasticity
  • Platelet-derived microparticles
  • Platelets
  • Serotonin
  • Traumatic brain injury

ASJC Scopus subject areas

  • Immunology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

Cite this

Dukhinova, M., Kuznetsova, I., Kopeikina, E., Veniaminova, E., Yung, A. W. Y., Veremeyko, T., ... Ponomarev, E. D. (Accepted/In press). Platelets mediate protective neuroinflammation and promote neuronal plasticity at the site of neuronal injury. Brain, Behavior, and Immunity. https://doi.org/10.1016/j.bbi.2018.09.009

Platelets mediate protective neuroinflammation and promote neuronal plasticity at the site of neuronal injury. / Dukhinova, Marina; Kuznetsova, Inna; Kopeikina, Ekaterina; Veniaminova, Ekaterina; Yung, Amanda W.Y.; Veremeyko, Tatyana; Levchuk, Kseniia; Barteneva, Natalie; Wing-Ho, Kenny Kam; Yung, Wing Ho; Liu, Julia Y.H.; Rudd, John; Yau, Sonata S.Y.; Anthony, Daniel C.; Strekalova, Tatyana; Ponomarev, Eugene D.

In: Brain, Behavior, and Immunity, 01.01.2018.

Research output: Contribution to journalArticle

Dukhinova, M, Kuznetsova, I, Kopeikina, E, Veniaminova, E, Yung, AWY, Veremeyko, T, Levchuk, K, Barteneva, N, Wing-Ho, KK, Yung, WH, Liu, JYH, Rudd, J, Yau, SSY, Anthony, DC, Strekalova, T & Ponomarev, ED 2018, 'Platelets mediate protective neuroinflammation and promote neuronal plasticity at the site of neuronal injury', Brain, Behavior, and Immunity. https://doi.org/10.1016/j.bbi.2018.09.009
Dukhinova, Marina ; Kuznetsova, Inna ; Kopeikina, Ekaterina ; Veniaminova, Ekaterina ; Yung, Amanda W.Y. ; Veremeyko, Tatyana ; Levchuk, Kseniia ; Barteneva, Natalie ; Wing-Ho, Kenny Kam ; Yung, Wing Ho ; Liu, Julia Y.H. ; Rudd, John ; Yau, Sonata S.Y. ; Anthony, Daniel C. ; Strekalova, Tatyana ; Ponomarev, Eugene D. / Platelets mediate protective neuroinflammation and promote neuronal plasticity at the site of neuronal injury. In: Brain, Behavior, and Immunity. 2018.
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