Plum fruit development occurs via gibberellin-sensitive and -insensitive DELLA repressors

Islam El-Sharkawy, Sherif Sherif, Mahboob Abdulla, Subramanian Jayasankar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fruit growth depends on highly coordinated hormonal activities. The phytohormone gibberellin (GA) promotes growth by triggering degradation of the growth-repressing DELLA proteins; however, the extent to which such proteins contribute to GA-mediated fruit development remains to be clarified. Three new plum genes encoding DELLA proteins, PslGAI, PslRGL and PslRGA were isolated and functionally characterized. Analysis of expression profile during fruit development suggested that PslDELLA are transcriptionally regulated during flower and fruit ontogeny with potential positive regulation by GA and ethylene, depending on organ and developmental stage. PslGAI and PslRGL deduced proteins contain all domains present in typical DELLA proteins. However, PslRGA exhibited a degenerated DELLA domain and subsequently lacks in GID1-DELLA interaction property. PslDELLA-overexpression in WT Arabidopsis caused dramatic disruption in overall growth including root length, stem elongation, plant architecture, flower structure, fertility, and considerable retardation in development due to dramatic distortion in GA-metabolic pathway. GA treatment enhanced PslGAI/PslRGL interaction with PslGID1 receptors, causing protein destabilization and relief of growth-restraining effect. By contrast, PslRGA protein was not degraded by GA due to its inability to interact with PslGID1. Relative to other PslDELLA-mutants, PslRGA-plants displayed stronger constitutive repressive growth that was irreversible by GA application. The present results describe additional complexities in GA-signalling during plum fruit development, which may be particularly important to optimize successful reproductive growth.

Original languageEnglish
Article numbere0169440
JournalPLoS One
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Gibberellins
plums
Fruits
gibberellins
fruiting
Fruit
Growth
Proteins
proteins
Plant Stems
flowers
Gene encoding
Plant Growth Regulators
fruits
plant architecture
stem elongation
Metabolic Networks and Pathways
Prunus domestica
Arabidopsis
plant hormones

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Plum fruit development occurs via gibberellin-sensitive and -insensitive DELLA repressors. / El-Sharkawy, Islam; Sherif, Sherif; Abdulla, Mahboob; Jayasankar, Subramanian.

In: PLoS One, Vol. 12, No. 1, e0169440, 01.01.2017.

Research output: Contribution to journalArticle

El-Sharkawy, Islam ; Sherif, Sherif ; Abdulla, Mahboob ; Jayasankar, Subramanian. / Plum fruit development occurs via gibberellin-sensitive and -insensitive DELLA repressors. In: PLoS One. 2017 ; Vol. 12, No. 1.
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