Poly-L-arginine modifications alter the organization and secretion of collagen in SKH1-E mice

Anuraag Boddupalli, Dana Akilbekova, Kaitlin M. Bratlie

Research output: Contribution to journalArticle

Abstract

Functionalized biomaterials interface with tissue upon implantation. There is a growing need to understand how materials properties influence this interaction so that efficient tissue engineering systems can be developed. In this study, we characterize collagen organization in response to functionalized glass beads implanted in SKH1-E mice. Poly-L-arginine (PLR) was modified with arginine derivatives to create a functionalized surface and was coated on glass beads. Tissue sections were removed 28 days post-implantation and were imaged using second harmonic generation (SHG) microscopy. These chemical modifications were able to alter the collagen distribution from highly aligned to disordered (17 ± 6 to 78 ± 1° full width at half-maximum (FWHM)) and the collagen III/I ratio (0.02 to 0.42). Principal component analysis (PCA) comparing the physical properties of the modifiers (e.g. hydrophobicity, molar volume, freely rotating bonds, polarizability) with the SHG analytically derived parameters (e.g. collagen III/I ratio, collagen orientation) was performed. Chemical properties of the PLR-like modifications including lipophilicity, along with the number of freely rotating bonds and the polarizability had significant effects on the collagen surrounding the implant, both in terms of collagen orientation as well as the production of collagen III. These findings demonstrate the possibility of tuning the foreign body response, in terms of collagen deposition and organization, to positively influence the acceptance of implanted biomaterials.

Original languageEnglish
Article number110143
JournalMaterials Science and Engineering C
Volume106
DOIs
Publication statusPublished - Jan 1 2020

Fingerprint

Arginine
secretions
collagens
Collagen
mice
Biocompatible Materials
Harmonic generation
Biomaterials
beads
implantation
harmonic generations
foreign bodies
Tissue
polyarginine
Glass
glass
tissue engineering
Chemical modification
Hydrophobicity
hydrophobicity

Keywords

  • Collagen
  • Poly-L-arginine
  • Second harmonic generation
  • SHG
  • SKH1-E mouse

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Poly-L-arginine modifications alter the organization and secretion of collagen in SKH1-E mice. / Boddupalli, Anuraag; Akilbekova, Dana; Bratlie, Kaitlin M.

In: Materials Science and Engineering C, Vol. 106, 110143, 01.01.2020.

Research output: Contribution to journalArticle

@article{533f5635d0e14fb4b39ad1f96a51fe26,
title = "Poly-L-arginine modifications alter the organization and secretion of collagen in SKH1-E mice",
abstract = "Functionalized biomaterials interface with tissue upon implantation. There is a growing need to understand how materials properties influence this interaction so that efficient tissue engineering systems can be developed. In this study, we characterize collagen organization in response to functionalized glass beads implanted in SKH1-E mice. Poly-L-arginine (PLR) was modified with arginine derivatives to create a functionalized surface and was coated on glass beads. Tissue sections were removed 28 days post-implantation and were imaged using second harmonic generation (SHG) microscopy. These chemical modifications were able to alter the collagen distribution from highly aligned to disordered (17 ± 6 to 78 ± 1° full width at half-maximum (FWHM)) and the collagen III/I ratio (0.02 to 0.42). Principal component analysis (PCA) comparing the physical properties of the modifiers (e.g. hydrophobicity, molar volume, freely rotating bonds, polarizability) with the SHG analytically derived parameters (e.g. collagen III/I ratio, collagen orientation) was performed. Chemical properties of the PLR-like modifications including lipophilicity, along with the number of freely rotating bonds and the polarizability had significant effects on the collagen surrounding the implant, both in terms of collagen orientation as well as the production of collagen III. These findings demonstrate the possibility of tuning the foreign body response, in terms of collagen deposition and organization, to positively influence the acceptance of implanted biomaterials.",
keywords = "Collagen, Poly-L-arginine, Second harmonic generation, SHG, SKH1-E mouse",
author = "Anuraag Boddupalli and Dana Akilbekova and Bratlie, {Kaitlin M.}",
year = "2020",
month = "1",
day = "1",
doi = "10.1016/j.msec.2019.110143",
language = "English",
volume = "106",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier",

}

TY - JOUR

T1 - Poly-L-arginine modifications alter the organization and secretion of collagen in SKH1-E mice

AU - Boddupalli, Anuraag

AU - Akilbekova, Dana

AU - Bratlie, Kaitlin M.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Functionalized biomaterials interface with tissue upon implantation. There is a growing need to understand how materials properties influence this interaction so that efficient tissue engineering systems can be developed. In this study, we characterize collagen organization in response to functionalized glass beads implanted in SKH1-E mice. Poly-L-arginine (PLR) was modified with arginine derivatives to create a functionalized surface and was coated on glass beads. Tissue sections were removed 28 days post-implantation and were imaged using second harmonic generation (SHG) microscopy. These chemical modifications were able to alter the collagen distribution from highly aligned to disordered (17 ± 6 to 78 ± 1° full width at half-maximum (FWHM)) and the collagen III/I ratio (0.02 to 0.42). Principal component analysis (PCA) comparing the physical properties of the modifiers (e.g. hydrophobicity, molar volume, freely rotating bonds, polarizability) with the SHG analytically derived parameters (e.g. collagen III/I ratio, collagen orientation) was performed. Chemical properties of the PLR-like modifications including lipophilicity, along with the number of freely rotating bonds and the polarizability had significant effects on the collagen surrounding the implant, both in terms of collagen orientation as well as the production of collagen III. These findings demonstrate the possibility of tuning the foreign body response, in terms of collagen deposition and organization, to positively influence the acceptance of implanted biomaterials.

AB - Functionalized biomaterials interface with tissue upon implantation. There is a growing need to understand how materials properties influence this interaction so that efficient tissue engineering systems can be developed. In this study, we characterize collagen organization in response to functionalized glass beads implanted in SKH1-E mice. Poly-L-arginine (PLR) was modified with arginine derivatives to create a functionalized surface and was coated on glass beads. Tissue sections were removed 28 days post-implantation and were imaged using second harmonic generation (SHG) microscopy. These chemical modifications were able to alter the collagen distribution from highly aligned to disordered (17 ± 6 to 78 ± 1° full width at half-maximum (FWHM)) and the collagen III/I ratio (0.02 to 0.42). Principal component analysis (PCA) comparing the physical properties of the modifiers (e.g. hydrophobicity, molar volume, freely rotating bonds, polarizability) with the SHG analytically derived parameters (e.g. collagen III/I ratio, collagen orientation) was performed. Chemical properties of the PLR-like modifications including lipophilicity, along with the number of freely rotating bonds and the polarizability had significant effects on the collagen surrounding the implant, both in terms of collagen orientation as well as the production of collagen III. These findings demonstrate the possibility of tuning the foreign body response, in terms of collagen deposition and organization, to positively influence the acceptance of implanted biomaterials.

KW - Collagen

KW - Poly-L-arginine

KW - Second harmonic generation

KW - SHG

KW - SKH1-E mouse

UR - http://www.scopus.com/inward/record.url?scp=85071866864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071866864&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2019.110143

DO - 10.1016/j.msec.2019.110143

M3 - Article

VL - 106

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

M1 - 110143

ER -