RFLP in the hsp70 gene, which encodes a major heat shock protein, was analyzed in rat strains with high and normal arterial blood pressures. Dimorphism in the sets of DNA fragments was revealed after hybridization of the hsp gene leader sequence, with rat DNA digested with BamHI restriction endonuclease. Type I RFLP was represented by fragments 12 200, 6500, 4100 and 1600 bp in size. Type II RFLP corresponded to the set that included fragments 12 200, 6500, 2900, 1600, and 1200 bp in size. Interstrain polymorphism was demonstrated for fragments 4100, 2900 and 1200 bp in size. Furthermore, analysis of different rat strains showed that the 2900- and 1200-bp fragments were linked and formed by cleavage of the 4100-bp fragment with restriction endonuclease. This polymorphism was probably caused by the point A → T mutation occurring in the BamHI recognition site located in the leader sequence of the hsp70 gene at a distance of +35 bp from the coding sequence. Examination of interstrain RFLP in the hsp70 gene indicated that the presence of 2900-bp fragments was not associated with hypertensive status in all experimental models of inherited arterial hypertension. This confirms the assumption on the genetic heterogeneity of this common disease.
|Number of pages||5|
|Journal||Russian Journal of Genetics|
|Publication status||Published - Mar 1 1998|
ASJC Scopus subject areas