Polymorphism of the gene for heat shock protein HSP70 in rat strains with normal and hypertensive status

V. A. Adarichev, G. M. Dymshits, A. A. Krivenko, Ya V. Ostapchuk, A. L. Market

Research output: Contribution to journalArticle

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Abstract

RFLP in the hsp70 gene encoding a major heat shock protein was analyzed in rat strains with high and normal arterial blood pressure. Dimorphism in the sets of DN A fragments was revealed after hybridization of the hsp gene leader sequence with rat DNA digested with BamHl restriction endonuclease. Type I RFLP was represented by the fragments of 12 200,6500,41 000 and 1600 bp in size. Type II RFLP corresponded to the set that included the fragments of 12 200, 6500, 2900, 1600, and 1200 bp in size. Interstrain polymorphism was demonstrated for the fragments of 4100, 2900 and 1200 bp. Furthermore, analysis of different rat strains showed that the 2900- and 1200-bp fragments were linked and formed by cleavage of the 4100-bp fragment with restriction endonuclease. This polymorphism was probably caused by the point A → T mutation occurring in the BamHl recognition site located in the leader sequence of the hsp70 gene at a distance of +35 bp from the coding sequence. Examination of interstrain RFLP in the hsp70 gene indicated that the presence of 2900-bp fragment was not associated with hypertensive status in all experimental models of inherited arterial hypertension. This confirmins the assumption on genetic heterogeneity of this common disease.

Original languageEnglish
Pages (from-to)393-398
Number of pages6
JournalGenetika
Volume34
Issue number3
Publication statusPublished - 1998
Externally publishedYes

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HSP70 Heat-Shock Proteins
Restriction Fragment Length Polymorphisms
DNA Restriction Enzymes
Genes
Genetic Heterogeneity
Heat-Shock Proteins
Arterial Pressure
Theoretical Models
Hypertension
Mutation
DNA

ASJC Scopus subject areas

  • Genetics

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Adarichev, V. A., Dymshits, G. M., Krivenko, A. A., Ostapchuk, Y. V., & Market, A. L. (1998). Polymorphism of the gene for heat shock protein HSP70 in rat strains with normal and hypertensive status. Genetika, 34(3), 393-398.

Polymorphism of the gene for heat shock protein HSP70 in rat strains with normal and hypertensive status. / Adarichev, V. A.; Dymshits, G. M.; Krivenko, A. A.; Ostapchuk, Ya V.; Market, A. L.

In: Genetika, Vol. 34, No. 3, 1998, p. 393-398.

Research output: Contribution to journalArticle

Adarichev, VA, Dymshits, GM, Krivenko, AA, Ostapchuk, YV & Market, AL 1998, 'Polymorphism of the gene for heat shock protein HSP70 in rat strains with normal and hypertensive status', Genetika, vol. 34, no. 3, pp. 393-398.
Adarichev VA, Dymshits GM, Krivenko AA, Ostapchuk YV, Market AL. Polymorphism of the gene for heat shock protein HSP70 in rat strains with normal and hypertensive status. Genetika. 1998;34(3):393-398.
Adarichev, V. A. ; Dymshits, G. M. ; Krivenko, A. A. ; Ostapchuk, Ya V. ; Market, A. L. / Polymorphism of the gene for heat shock protein HSP70 in rat strains with normal and hypertensive status. In: Genetika. 1998 ; Vol. 34, No. 3. pp. 393-398.
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