Porous silicon membrane for investigation of transmembrane proteins

Khalid Hasan Tantawi, Bakhrom Berdiev, Ramon Cerro, John D. Williams

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This article presents the assembly and signal transduction of an artificial biological membrane suspended on a thin porous silicon template. The electrochemically-fabricated porous silicon membrane has average pore diameters in the range 0.50-2 μm and dimensions of about 200 × 200 × 3 μm3 and may be batch fabricated in large arrays for combinatorial testing. Biological membranes may be deposited on one or both sides of this template are fully accessible for studies using electrochemical impedance spectroscopy. Initial results using a two probe impedance measurement clearly show a significant impedance change between the porous silicon structure and the lipid bilayer. Furthermore, there is a clear reduction in the impedance of lipid bilayer when fused with a transmembrane ion channel protein. The photoluminescence and biodegradability properties of porous silicon in addition to lower cost and ease of fabrication make it superior over e-beam patterned silicon structures used in previous works, and thus suitable for in vivo monitoring.

Original languageEnglish
Pages (from-to)72-80
Number of pages9
JournalSuperlattices and Microstructures
Volume58
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Porous silicon
porous silicon
membranes
proteins
Proteins
Membranes
Biological membranes
Lipid bilayers
impedance
lipids
templates
biodegradability
Signal transduction
Biodegradability
impedance measurement
Silicon
Electrochemical impedance spectroscopy
Ion Channels
Photoluminescence
assembly

Keywords

  • Artificial cell membranes
  • Porous silicon
  • Supported lipid membranes
  • Two-chamber microstructure

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Materials Science(all)

Cite this

Porous silicon membrane for investigation of transmembrane proteins. / Tantawi, Khalid Hasan; Berdiev, Bakhrom; Cerro, Ramon; Williams, John D.

In: Superlattices and Microstructures, Vol. 58, 2013, p. 72-80.

Research output: Contribution to journalArticle

Tantawi, Khalid Hasan ; Berdiev, Bakhrom ; Cerro, Ramon ; Williams, John D. / Porous silicon membrane for investigation of transmembrane proteins. In: Superlattices and Microstructures. 2013 ; Vol. 58. pp. 72-80.
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