Progenitor cells of the testosterone-producing Leydig cells revealed

Michail S. Davidoff, Ralf Middendorff, Grigori Enikolopov, Dieter Riethmacher, Adolf F. Holstein, Dieter Müller

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

The cells responsible for production of the male sex hormone testosterone, the Leydig cells of the testis, are post-mitotic cells with neuroendocrine characteristics. Their origin during ontogeny and regeneration processes is still a matter of debate. Here, we show that cells of testicular blood vessels, namely vascular smooth muscle cells and pericytes, are the progenitors of Leydig cells. Resembling stem cells of the nervous system, the Leydig cell progenitors are characterized by the expression of nestin. Using an in vivo model to induce and monitor the synchronized generation of a completely new Leydig cell population in adult rats, we demonstrate specific proliferation of vascular progenitors and their subsequent transdifferentiation into steroidogenic Leydig cells which, in addition, rapidly acquire neuronal and glial properties. These findings, shown to be representative also for ontogenetic Leydig cell formation and for the human testis, provide further evidence that cellular components of blood vessels can act as progenitor cells for organogenesis and repair.

Original languageEnglish
Pages (from-to)935-944
Number of pages10
JournalJournal of Cell Biology
Volume167
Issue number5
DOIs
Publication statusPublished - Dec 5 2004
Externally publishedYes

Fingerprint

Leydig Cells
Testosterone
Stem Cells
Blood Vessels
Testis
Nestin
Neuroendocrine Cells
Pericytes
Organogenesis
Gonadal Steroid Hormones
Vascular Smooth Muscle
Neuroglia
Nervous System
Smooth Muscle Myocytes
Regeneration
Population

ASJC Scopus subject areas

  • Cell Biology

Cite this

Davidoff, M. S., Middendorff, R., Enikolopov, G., Riethmacher, D., Holstein, A. F., & Müller, D. (2004). Progenitor cells of the testosterone-producing Leydig cells revealed. Journal of Cell Biology, 167(5), 935-944. https://doi.org/10.1083/jcb.200409107

Progenitor cells of the testosterone-producing Leydig cells revealed. / Davidoff, Michail S.; Middendorff, Ralf; Enikolopov, Grigori; Riethmacher, Dieter; Holstein, Adolf F.; Müller, Dieter.

In: Journal of Cell Biology, Vol. 167, No. 5, 05.12.2004, p. 935-944.

Research output: Contribution to journalArticle

Davidoff, MS, Middendorff, R, Enikolopov, G, Riethmacher, D, Holstein, AF & Müller, D 2004, 'Progenitor cells of the testosterone-producing Leydig cells revealed', Journal of Cell Biology, vol. 167, no. 5, pp. 935-944. https://doi.org/10.1083/jcb.200409107
Davidoff MS, Middendorff R, Enikolopov G, Riethmacher D, Holstein AF, Müller D. Progenitor cells of the testosterone-producing Leydig cells revealed. Journal of Cell Biology. 2004 Dec 5;167(5):935-944. https://doi.org/10.1083/jcb.200409107
Davidoff, Michail S. ; Middendorff, Ralf ; Enikolopov, Grigori ; Riethmacher, Dieter ; Holstein, Adolf F. ; Müller, Dieter. / Progenitor cells of the testosterone-producing Leydig cells revealed. In: Journal of Cell Biology. 2004 ; Vol. 167, No. 5. pp. 935-944.
@article{722dd57e5a874881a50b4a53e4d601fb,
title = "Progenitor cells of the testosterone-producing Leydig cells revealed",
abstract = "The cells responsible for production of the male sex hormone testosterone, the Leydig cells of the testis, are post-mitotic cells with neuroendocrine characteristics. Their origin during ontogeny and regeneration processes is still a matter of debate. Here, we show that cells of testicular blood vessels, namely vascular smooth muscle cells and pericytes, are the progenitors of Leydig cells. Resembling stem cells of the nervous system, the Leydig cell progenitors are characterized by the expression of nestin. Using an in vivo model to induce and monitor the synchronized generation of a completely new Leydig cell population in adult rats, we demonstrate specific proliferation of vascular progenitors and their subsequent transdifferentiation into steroidogenic Leydig cells which, in addition, rapidly acquire neuronal and glial properties. These findings, shown to be representative also for ontogenetic Leydig cell formation and for the human testis, provide further evidence that cellular components of blood vessels can act as progenitor cells for organogenesis and repair.",
author = "Davidoff, {Michail S.} and Ralf Middendorff and Grigori Enikolopov and Dieter Riethmacher and Holstein, {Adolf F.} and Dieter M{\"u}ller",
year = "2004",
month = "12",
day = "5",
doi = "10.1083/jcb.200409107",
language = "English",
volume = "167",
pages = "935--944",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - Progenitor cells of the testosterone-producing Leydig cells revealed

AU - Davidoff, Michail S.

AU - Middendorff, Ralf

AU - Enikolopov, Grigori

AU - Riethmacher, Dieter

AU - Holstein, Adolf F.

AU - Müller, Dieter

PY - 2004/12/5

Y1 - 2004/12/5

N2 - The cells responsible for production of the male sex hormone testosterone, the Leydig cells of the testis, are post-mitotic cells with neuroendocrine characteristics. Their origin during ontogeny and regeneration processes is still a matter of debate. Here, we show that cells of testicular blood vessels, namely vascular smooth muscle cells and pericytes, are the progenitors of Leydig cells. Resembling stem cells of the nervous system, the Leydig cell progenitors are characterized by the expression of nestin. Using an in vivo model to induce and monitor the synchronized generation of a completely new Leydig cell population in adult rats, we demonstrate specific proliferation of vascular progenitors and their subsequent transdifferentiation into steroidogenic Leydig cells which, in addition, rapidly acquire neuronal and glial properties. These findings, shown to be representative also for ontogenetic Leydig cell formation and for the human testis, provide further evidence that cellular components of blood vessels can act as progenitor cells for organogenesis and repair.

AB - The cells responsible for production of the male sex hormone testosterone, the Leydig cells of the testis, are post-mitotic cells with neuroendocrine characteristics. Their origin during ontogeny and regeneration processes is still a matter of debate. Here, we show that cells of testicular blood vessels, namely vascular smooth muscle cells and pericytes, are the progenitors of Leydig cells. Resembling stem cells of the nervous system, the Leydig cell progenitors are characterized by the expression of nestin. Using an in vivo model to induce and monitor the synchronized generation of a completely new Leydig cell population in adult rats, we demonstrate specific proliferation of vascular progenitors and their subsequent transdifferentiation into steroidogenic Leydig cells which, in addition, rapidly acquire neuronal and glial properties. These findings, shown to be representative also for ontogenetic Leydig cell formation and for the human testis, provide further evidence that cellular components of blood vessels can act as progenitor cells for organogenesis and repair.

UR - http://www.scopus.com/inward/record.url?scp=10344249405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10344249405&partnerID=8YFLogxK

U2 - 10.1083/jcb.200409107

DO - 10.1083/jcb.200409107

M3 - Article

C2 - 15569711

AN - SCOPUS:10344249405

VL - 167

SP - 935

EP - 944

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 5

ER -