Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins

Songsak Tongchusak, Vladimir Brusic, Sansanee C. Chaiyaroj

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Candida albicans is one of the most important opportunistic dimorphic fungi responsible for hospital acquired fungal infection in humans. Candida infection rarely occurs in healthy individuals but it is frequently associated with patients who suffer from acquired immunodeficiency syndromes. To date, there is no effective vaccine against this fungal infection. Herein we demonstrated the use of immunomics to characterize promiscuous T cell epitope of C. albicans virulence factors by utilizing CandiVF, a C. albicans database previously constructed to be equipped with protein sequence analysis tool, three dimensional structure visualization software, sequence variable analysis program and Hotspot Hunter epitope prediction tool. Secretory aspartyl proteinase (Sap) family was chosen as a model to validate the Hotspot Hunter prediction. Analysis of Saps1-10 protein entries from CandiVF database revealed that a consensus T cell epitope was located at the C-terminal region of Saps1-10. The result of the in silico prediction was subsequently validated by conventional immunological methods. By using overlapping peptides span the predicted consensus T cell epitopes of Saps1-10 as stimulators, it was demonstrated that peptides S6 and S7 could stimulate PBMC proliferation in 9 of 12 blood donors. Interestingly, S2, the predicted T cell epitope of Sap2, was able to induce proliferation of all donors' PBMC. ELISpot assay for the detection of gamma-interferon producing clones confirmed that the peptide S2 actually stimulated T cell proliferation. The results suggest that S2 might be a potential candidate for vaccine development against C. albicans infection or to be utilized as an adjuvant to stimulate the pre-existing CD4+ T cell in other vaccine development.

Original languageEnglish
Pages (from-to)467-473
Number of pages7
JournalInfection, Genetics and Evolution
Volume8
Issue number4
DOIs
Publication statusPublished - Jul 2008
Externally publishedYes

Fingerprint

T-Lymphocyte Epitopes
Candida albicans
epitopes
vaccine
T-lymphocytes
peptide
protein
prediction
Vaccines
Mycoses
Peptides
Proteins
proteins
ethyl-2-methylthio-4-methyl-5-pyrimidine carboxylate
vaccine development
Databases
peptides
mononuclear leukocytes
Aspartic Acid Proteases
T-Lymphocytes

Keywords

  • Antigenic prediction
  • Candida albicans
  • Immunomics
  • Interferon ELISpot assay
  • PBMC proliferation
  • Secretory aspartyl proteinase
  • T cell epitope

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Microbiology
  • Infectious Diseases

Cite this

Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins. / Tongchusak, Songsak; Brusic, Vladimir; Chaiyaroj, Sansanee C.

In: Infection, Genetics and Evolution, Vol. 8, No. 4, 07.2008, p. 467-473.

Research output: Contribution to journalArticle

Tongchusak, Songsak ; Brusic, Vladimir ; Chaiyaroj, Sansanee C. / Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins. In: Infection, Genetics and Evolution. 2008 ; Vol. 8, No. 4. pp. 467-473.
@article{460a9227e0234d619665321bdbefeb1f,
title = "Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins",
abstract = "Candida albicans is one of the most important opportunistic dimorphic fungi responsible for hospital acquired fungal infection in humans. Candida infection rarely occurs in healthy individuals but it is frequently associated with patients who suffer from acquired immunodeficiency syndromes. To date, there is no effective vaccine against this fungal infection. Herein we demonstrated the use of immunomics to characterize promiscuous T cell epitope of C. albicans virulence factors by utilizing CandiVF, a C. albicans database previously constructed to be equipped with protein sequence analysis tool, three dimensional structure visualization software, sequence variable analysis program and Hotspot Hunter epitope prediction tool. Secretory aspartyl proteinase (Sap) family was chosen as a model to validate the Hotspot Hunter prediction. Analysis of Saps1-10 protein entries from CandiVF database revealed that a consensus T cell epitope was located at the C-terminal region of Saps1-10. The result of the in silico prediction was subsequently validated by conventional immunological methods. By using overlapping peptides span the predicted consensus T cell epitopes of Saps1-10 as stimulators, it was demonstrated that peptides S6 and S7 could stimulate PBMC proliferation in 9 of 12 blood donors. Interestingly, S2, the predicted T cell epitope of Sap2, was able to induce proliferation of all donors' PBMC. ELISpot assay for the detection of gamma-interferon producing clones confirmed that the peptide S2 actually stimulated T cell proliferation. The results suggest that S2 might be a potential candidate for vaccine development against C. albicans infection or to be utilized as an adjuvant to stimulate the pre-existing CD4+ T cell in other vaccine development.",
keywords = "Antigenic prediction, Candida albicans, Immunomics, Interferon ELISpot assay, PBMC proliferation, Secretory aspartyl proteinase, T cell epitope",
author = "Songsak Tongchusak and Vladimir Brusic and Chaiyaroj, {Sansanee C.}",
year = "2008",
month = "7",
doi = "10.1016/j.meegid.2007.09.006",
language = "English",
volume = "8",
pages = "467--473",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Promiscuous T cell epitope prediction of Candida albicans secretory aspartyl protienase family of proteins

AU - Tongchusak, Songsak

AU - Brusic, Vladimir

AU - Chaiyaroj, Sansanee C.

PY - 2008/7

Y1 - 2008/7

N2 - Candida albicans is one of the most important opportunistic dimorphic fungi responsible for hospital acquired fungal infection in humans. Candida infection rarely occurs in healthy individuals but it is frequently associated with patients who suffer from acquired immunodeficiency syndromes. To date, there is no effective vaccine against this fungal infection. Herein we demonstrated the use of immunomics to characterize promiscuous T cell epitope of C. albicans virulence factors by utilizing CandiVF, a C. albicans database previously constructed to be equipped with protein sequence analysis tool, three dimensional structure visualization software, sequence variable analysis program and Hotspot Hunter epitope prediction tool. Secretory aspartyl proteinase (Sap) family was chosen as a model to validate the Hotspot Hunter prediction. Analysis of Saps1-10 protein entries from CandiVF database revealed that a consensus T cell epitope was located at the C-terminal region of Saps1-10. The result of the in silico prediction was subsequently validated by conventional immunological methods. By using overlapping peptides span the predicted consensus T cell epitopes of Saps1-10 as stimulators, it was demonstrated that peptides S6 and S7 could stimulate PBMC proliferation in 9 of 12 blood donors. Interestingly, S2, the predicted T cell epitope of Sap2, was able to induce proliferation of all donors' PBMC. ELISpot assay for the detection of gamma-interferon producing clones confirmed that the peptide S2 actually stimulated T cell proliferation. The results suggest that S2 might be a potential candidate for vaccine development against C. albicans infection or to be utilized as an adjuvant to stimulate the pre-existing CD4+ T cell in other vaccine development.

AB - Candida albicans is one of the most important opportunistic dimorphic fungi responsible for hospital acquired fungal infection in humans. Candida infection rarely occurs in healthy individuals but it is frequently associated with patients who suffer from acquired immunodeficiency syndromes. To date, there is no effective vaccine against this fungal infection. Herein we demonstrated the use of immunomics to characterize promiscuous T cell epitope of C. albicans virulence factors by utilizing CandiVF, a C. albicans database previously constructed to be equipped with protein sequence analysis tool, three dimensional structure visualization software, sequence variable analysis program and Hotspot Hunter epitope prediction tool. Secretory aspartyl proteinase (Sap) family was chosen as a model to validate the Hotspot Hunter prediction. Analysis of Saps1-10 protein entries from CandiVF database revealed that a consensus T cell epitope was located at the C-terminal region of Saps1-10. The result of the in silico prediction was subsequently validated by conventional immunological methods. By using overlapping peptides span the predicted consensus T cell epitopes of Saps1-10 as stimulators, it was demonstrated that peptides S6 and S7 could stimulate PBMC proliferation in 9 of 12 blood donors. Interestingly, S2, the predicted T cell epitope of Sap2, was able to induce proliferation of all donors' PBMC. ELISpot assay for the detection of gamma-interferon producing clones confirmed that the peptide S2 actually stimulated T cell proliferation. The results suggest that S2 might be a potential candidate for vaccine development against C. albicans infection or to be utilized as an adjuvant to stimulate the pre-existing CD4+ T cell in other vaccine development.

KW - Antigenic prediction

KW - Candida albicans

KW - Immunomics

KW - Interferon ELISpot assay

KW - PBMC proliferation

KW - Secretory aspartyl proteinase

KW - T cell epitope

UR - http://www.scopus.com/inward/record.url?scp=43649103417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43649103417&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2007.09.006

DO - 10.1016/j.meegid.2007.09.006

M3 - Article

VL - 8

SP - 467

EP - 473

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

IS - 4

ER -