Proposal for assessment of the antimicrobial efficacy of undiluted medical honey: Using a standardized phase 2/step 2 in vitro stainless steel disc carrier test model

Anna Maria Goerdt, Ojan Assadian, Behzad Razavi, Dorothee Igelbrink-Holter, Arne Simon, Nils Olaf Hübner, Lars Ivo Partecke, Anara Zhumadilova, Claus Dieter Heidecke, Axel Kramer

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: The antimicrobial efficacy of medical honeys used for professional wound care was published previously. So far, all in vitro antimicrobial tests performed have been conducted using non-standardized methods and used diluted medical honey. A standardized, reproducible phase 2/step 2 in vitro disc carrier model is proposed allowing testing the antimicrobial efficacy of undiluted medical honey. Methods: Using a standardized disc carrier model, the log10 Reduction Factor (RF) of undiluted medical honey was determined for MSSA, MRSA, VRE, Pseudomonas aeruginosa, and Candida albicans, with and without protein challenge. Results: An antimicrobial efficacy was observed after 1h. Without organic challenge, log10RF >5 within 24h was achieved for all tested microorganisms, except for Staphylococcus aureus (log10RF: 4.8/24h). Challenged with 10% bovine serum albumin, a similar pattern was observed at 24h. The most susceptible organism was C. albicans (log10RF of >3/5min). Over an exposure time of 48h, the tested medical honey's antimicrobial activity under protein challenge achieved log10RFs >6 for C. albicans and >7log10 for all tested bacteria. Conclusions: It was demonstrated that the proposed standardized phase 2/step 2 in vitro stainless steel disc carrier test model is applicable for testing the antimicrobial activity of highly viscous and undiluted compounds such as medical honey. Undiluted medical honey exhibits a strong antimicrobial efficacy even under protein challenge after 1h, which increases to >6log10 for C. albicans, and >7log10 for Gr+ and Gr- bacteria.

Original languageEnglish
Pages (from-to)20-24
Number of pages5
JournalWound Medicine
Volume1
DOIs
Publication statusPublished - Jul 1 2013

Keywords

  • Antimicrobial
  • Candida albicans
  • In vitro
  • MRSA
  • Medical honey
  • Medihoney
  • Microbicidal

ASJC Scopus subject areas

  • Surgery

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