Proposal for assessment of the antimicrobial efficacy of undiluted medical honey

Using a standardized phase 2/step 2 in vitro stainless steel disc carrier test model

Anna Maria Goerdt, Ojan Assadian, Behzad Razavi, Dorothee Igelbrink-Holter, Arne Simon, Nils Olaf Hübner, Lars Ivo Partecke, Anara Zhumadilova, Claus Dieter Heidecke, Axel Kramer

    Research output: Contribution to journalArticle

    Abstract

    Purpose: The antimicrobial efficacy of medical honeys used for professional wound care was published previously. So far, all in vitro antimicrobial tests performed have been conducted using non-standardized methods and used diluted medical honey. A standardized, reproducible phase 2/step 2 in vitro disc carrier model is proposed allowing testing the antimicrobial efficacy of undiluted medical honey. Methods: Using a standardized disc carrier model, the log10 Reduction Factor (RF) of undiluted medical honey was determined for MSSA, MRSA, VRE, Pseudomonas aeruginosa, and Candida albicans, with and without protein challenge. Results: An antimicrobial efficacy was observed after 1h. Without organic challenge, log10RF >5 within 24h was achieved for all tested microorganisms, except for Staphylococcus aureus (log10RF: 4.8/24h). Challenged with 10% bovine serum albumin, a similar pattern was observed at 24h. The most susceptible organism was C. albicans (log10RF of >3/5min). Over an exposure time of 48h, the tested medical honey's antimicrobial activity under protein challenge achieved log10RFs >6 for C. albicans and >7log10 for all tested bacteria. Conclusions: It was demonstrated that the proposed standardized phase 2/step 2 in vitro stainless steel disc carrier test model is applicable for testing the antimicrobial activity of highly viscous and undiluted compounds such as medical honey. Undiluted medical honey exhibits a strong antimicrobial efficacy even under protein challenge after 1h, which increases to >6log10 for C. albicans, and >7log10 for Gr+ and Gr- bacteria.

    Original languageEnglish
    Pages (from-to)20-24
    Number of pages5
    JournalWound Medicine
    Volume1
    DOIs
    Publication statusPublished - Jul 2013

    Fingerprint

    Honey
    Stainless Steel
    Candida albicans
    Bacteria
    Proteins
    Methicillin-Resistant Staphylococcus aureus
    Bovine Serum Albumin
    In Vitro Techniques
    Pseudomonas aeruginosa
    Staphylococcus aureus
    Wounds and Injuries

    Keywords

    • Antimicrobial
    • Candida albicans
    • In vitro
    • Medical honey
    • Medihoney
    • Microbicidal
    • MRSA

    ASJC Scopus subject areas

    • Surgery

    Cite this

    Proposal for assessment of the antimicrobial efficacy of undiluted medical honey : Using a standardized phase 2/step 2 in vitro stainless steel disc carrier test model. / Goerdt, Anna Maria; Assadian, Ojan; Razavi, Behzad; Igelbrink-Holter, Dorothee; Simon, Arne; Hübner, Nils Olaf; Partecke, Lars Ivo; Zhumadilova, Anara; Heidecke, Claus Dieter; Kramer, Axel.

    In: Wound Medicine, Vol. 1, 07.2013, p. 20-24.

    Research output: Contribution to journalArticle

    Goerdt, Anna Maria ; Assadian, Ojan ; Razavi, Behzad ; Igelbrink-Holter, Dorothee ; Simon, Arne ; Hübner, Nils Olaf ; Partecke, Lars Ivo ; Zhumadilova, Anara ; Heidecke, Claus Dieter ; Kramer, Axel. / Proposal for assessment of the antimicrobial efficacy of undiluted medical honey : Using a standardized phase 2/step 2 in vitro stainless steel disc carrier test model. In: Wound Medicine. 2013 ; Vol. 1. pp. 20-24.
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    abstract = "Purpose: The antimicrobial efficacy of medical honeys used for professional wound care was published previously. So far, all in vitro antimicrobial tests performed have been conducted using non-standardized methods and used diluted medical honey. A standardized, reproducible phase 2/step 2 in vitro disc carrier model is proposed allowing testing the antimicrobial efficacy of undiluted medical honey. Methods: Using a standardized disc carrier model, the log10 Reduction Factor (RF) of undiluted medical honey was determined for MSSA, MRSA, VRE, Pseudomonas aeruginosa, and Candida albicans, with and without protein challenge. Results: An antimicrobial efficacy was observed after 1h. Without organic challenge, log10RF >5 within 24h was achieved for all tested microorganisms, except for Staphylococcus aureus (log10RF: 4.8/24h). Challenged with 10{\%} bovine serum albumin, a similar pattern was observed at 24h. The most susceptible organism was C. albicans (log10RF of >3/5min). Over an exposure time of 48h, the tested medical honey's antimicrobial activity under protein challenge achieved log10RFs >6 for C. albicans and >7log10 for all tested bacteria. Conclusions: It was demonstrated that the proposed standardized phase 2/step 2 in vitro stainless steel disc carrier test model is applicable for testing the antimicrobial activity of highly viscous and undiluted compounds such as medical honey. Undiluted medical honey exhibits a strong antimicrobial efficacy even under protein challenge after 1h, which increases to >6log10 for C. albicans, and >7log10 for Gr+ and Gr- bacteria.",
    keywords = "Antimicrobial, Candida albicans, In vitro, Medical honey, Medihoney, Microbicidal, MRSA",
    author = "Goerdt, {Anna Maria} and Ojan Assadian and Behzad Razavi and Dorothee Igelbrink-Holter and Arne Simon and H{\"u}bner, {Nils Olaf} and Partecke, {Lars Ivo} and Anara Zhumadilova and Heidecke, {Claus Dieter} and Axel Kramer",
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    T1 - Proposal for assessment of the antimicrobial efficacy of undiluted medical honey

    T2 - Using a standardized phase 2/step 2 in vitro stainless steel disc carrier test model

    AU - Goerdt, Anna Maria

    AU - Assadian, Ojan

    AU - Razavi, Behzad

    AU - Igelbrink-Holter, Dorothee

    AU - Simon, Arne

    AU - Hübner, Nils Olaf

    AU - Partecke, Lars Ivo

    AU - Zhumadilova, Anara

    AU - Heidecke, Claus Dieter

    AU - Kramer, Axel

    PY - 2013/7

    Y1 - 2013/7

    N2 - Purpose: The antimicrobial efficacy of medical honeys used for professional wound care was published previously. So far, all in vitro antimicrobial tests performed have been conducted using non-standardized methods and used diluted medical honey. A standardized, reproducible phase 2/step 2 in vitro disc carrier model is proposed allowing testing the antimicrobial efficacy of undiluted medical honey. Methods: Using a standardized disc carrier model, the log10 Reduction Factor (RF) of undiluted medical honey was determined for MSSA, MRSA, VRE, Pseudomonas aeruginosa, and Candida albicans, with and without protein challenge. Results: An antimicrobial efficacy was observed after 1h. Without organic challenge, log10RF >5 within 24h was achieved for all tested microorganisms, except for Staphylococcus aureus (log10RF: 4.8/24h). Challenged with 10% bovine serum albumin, a similar pattern was observed at 24h. The most susceptible organism was C. albicans (log10RF of >3/5min). Over an exposure time of 48h, the tested medical honey's antimicrobial activity under protein challenge achieved log10RFs >6 for C. albicans and >7log10 for all tested bacteria. Conclusions: It was demonstrated that the proposed standardized phase 2/step 2 in vitro stainless steel disc carrier test model is applicable for testing the antimicrobial activity of highly viscous and undiluted compounds such as medical honey. Undiluted medical honey exhibits a strong antimicrobial efficacy even under protein challenge after 1h, which increases to >6log10 for C. albicans, and >7log10 for Gr+ and Gr- bacteria.

    AB - Purpose: The antimicrobial efficacy of medical honeys used for professional wound care was published previously. So far, all in vitro antimicrobial tests performed have been conducted using non-standardized methods and used diluted medical honey. A standardized, reproducible phase 2/step 2 in vitro disc carrier model is proposed allowing testing the antimicrobial efficacy of undiluted medical honey. Methods: Using a standardized disc carrier model, the log10 Reduction Factor (RF) of undiluted medical honey was determined for MSSA, MRSA, VRE, Pseudomonas aeruginosa, and Candida albicans, with and without protein challenge. Results: An antimicrobial efficacy was observed after 1h. Without organic challenge, log10RF >5 within 24h was achieved for all tested microorganisms, except for Staphylococcus aureus (log10RF: 4.8/24h). Challenged with 10% bovine serum albumin, a similar pattern was observed at 24h. The most susceptible organism was C. albicans (log10RF of >3/5min). Over an exposure time of 48h, the tested medical honey's antimicrobial activity under protein challenge achieved log10RFs >6 for C. albicans and >7log10 for all tested bacteria. Conclusions: It was demonstrated that the proposed standardized phase 2/step 2 in vitro stainless steel disc carrier test model is applicable for testing the antimicrobial activity of highly viscous and undiluted compounds such as medical honey. Undiluted medical honey exhibits a strong antimicrobial efficacy even under protein challenge after 1h, which increases to >6log10 for C. albicans, and >7log10 for Gr+ and Gr- bacteria.

    KW - Antimicrobial

    KW - Candida albicans

    KW - In vitro

    KW - Medical honey

    KW - Medihoney

    KW - Microbicidal

    KW - MRSA

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