Protein zero gene expression is regulated by the glial transcription factor Sox10

Reto I. Peirano, Derk E. Goerich, Dieter Riethmacher, Michael Wegner

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

Myelinating glia express high levels of a unique set of genes which code for structural proteins of the myelin sheath. Few transcription factors have so far been implicated in the regulation of any myelin gene. Here we show that the protein zero (P0) gene, a myelin gene exclusively expressed in the Schwann cell lineage of the peripheral nervous system, is controlled in its expression by the high-mobility-group domain protein Sox10 both in tissue culture and in vivo. Induction of wild-type Sox10, but not of other transcription factors or Sox10 mutants, strongly increased endogenous P0 expression in tissue culture. This activation was mediated by the P0 promoter, which was stimulated by Sox10 in transient transfections. Detailed analyses revealed the involvement of a proximal and a distal promoter region. The distal region functioned only in conjunction with the proximal one and contained a single Sox consensus binding site, which accounted for most of its activity. In contrast, the proximal region mediated Sox10 responsiveness on its own. It contained multiple binding sites for Sox proteins, with two high-affinity sites being the most significant. P0 expression also depended on Sox10 in vivo, as evident from the analysis of Schwann cell precursors in mouse embryos with Sox10 mutation at day 12.5 of embryogenesis. To our knowledge this is the most conclusive link to date between a glial transcription factor and cell-specific activation of myelin gene expression.

Original languageEnglish
Pages (from-to)3198-3209
Number of pages12
JournalMolecular and Cellular Biology
Volume20
Issue number9
DOIs
Publication statusPublished - May 2000
Externally publishedYes

Fingerprint

Myelin Sheath
Neuroglia
Transcription Factors
Gene Expression
Schwann Cells
Genes
Proteins
Myelin P0 Protein
Binding Sites
High Mobility Group Proteins
Peripheral Nervous System
Cell Lineage
Genetic Promoter Regions
Embryonic Development
Transfection
Embryonic Structures
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Protein zero gene expression is regulated by the glial transcription factor Sox10. / Peirano, Reto I.; Goerich, Derk E.; Riethmacher, Dieter; Wegner, Michael.

In: Molecular and Cellular Biology, Vol. 20, No. 9, 05.2000, p. 3198-3209.

Research output: Contribution to journalArticle

Peirano, Reto I. ; Goerich, Derk E. ; Riethmacher, Dieter ; Wegner, Michael. / Protein zero gene expression is regulated by the glial transcription factor Sox10. In: Molecular and Cellular Biology. 2000 ; Vol. 20, No. 9. pp. 3198-3209.
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