Proteomic analysis of cellular response induced by boron neutron capture reaction in human squamous cell carcinoma SAS cells

Akira Sato, Tasuku Itoh, Shoji Imamichi, Sota Kikuhara, Hiroaki Fujimori, Takahisa Hirai, Soichiro Saito, Yoshinori Sakurai, Hiroki Tanaka, Hiroyuki Nakamura, Minoru Suzuki, Yasufumi Murakami, Diaz Baiseitov, Kulzhan Berikkhanova, Zhaxybay Zhumadilov, Yoshio Imahori, Jun Itami, Koji Ono, Shinichiro Masunaga, Mitsuko Masutani

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To understand the mechanism of cell death induced by boron neutron capture reaction (BNCR), we performed proteome analyses of human squamous tumor SAS cells after BNCR. Cells were irradiated with thermal neutron beam at KUR after incubation under boronophenylalanine (BPA)(+) and BPA(-) conditions. BNCR mainly induced typical apoptosis in SAS cells 24 h post-irradiation. Proteomic analysis in SAS cells suggested that proteins functioning in endoplasmic reticulum, DNA repair, and RNA processing showed dynamic changes at early phase after BNCR and could be involved in the regulation of cellular response to BNCR. We found that the BNCR induces fragments of endoplasmic reticulum-localized lymphoid-restricted protein (LRMP). The fragmentation of LRMP was also observed in the rat tumor graft model 20 hours after BNCT treatment carried out at the National Nuclear Center of the Republic of Kazakhstan. These data suggest that dynamic changes of LRMP could be involved during cellular response to BNCR.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalApplied Radiation and Isotopes
Volume106
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

SAS
boron
cancer
neutrons
cells
endoplasmic reticulum
tumors
proteome
Kazakhstan
proteins
neutron beams
apoptosis
thermal neutrons
death
rats
fragmentation
deoxyribonucleic acid
fragments
irradiation

Keywords

  • Apoptosis
  • Boron neutron capture reaction (BNCR)
  • Lymphoid-restricted protein (LRMP)
  • Proteome analysis

ASJC Scopus subject areas

  • Radiation

Cite this

Proteomic analysis of cellular response induced by boron neutron capture reaction in human squamous cell carcinoma SAS cells. / Sato, Akira; Itoh, Tasuku; Imamichi, Shoji; Kikuhara, Sota; Fujimori, Hiroaki; Hirai, Takahisa; Saito, Soichiro; Sakurai, Yoshinori; Tanaka, Hiroki; Nakamura, Hiroyuki; Suzuki, Minoru; Murakami, Yasufumi; Baiseitov, Diaz; Berikkhanova, Kulzhan; Zhumadilov, Zhaxybay; Imahori, Yoshio; Itami, Jun; Ono, Koji; Masunaga, Shinichiro; Masutani, Mitsuko.

In: Applied Radiation and Isotopes, Vol. 106, 01.12.2015, p. 213-219.

Research output: Contribution to journalArticle

Sato, A, Itoh, T, Imamichi, S, Kikuhara, S, Fujimori, H, Hirai, T, Saito, S, Sakurai, Y, Tanaka, H, Nakamura, H, Suzuki, M, Murakami, Y, Baiseitov, D, Berikkhanova, K, Zhumadilov, Z, Imahori, Y, Itami, J, Ono, K, Masunaga, S & Masutani, M 2015, 'Proteomic analysis of cellular response induced by boron neutron capture reaction in human squamous cell carcinoma SAS cells', Applied Radiation and Isotopes, vol. 106, pp. 213-219. https://doi.org/10.1016/j.apradiso.2015.08.001
Sato, Akira ; Itoh, Tasuku ; Imamichi, Shoji ; Kikuhara, Sota ; Fujimori, Hiroaki ; Hirai, Takahisa ; Saito, Soichiro ; Sakurai, Yoshinori ; Tanaka, Hiroki ; Nakamura, Hiroyuki ; Suzuki, Minoru ; Murakami, Yasufumi ; Baiseitov, Diaz ; Berikkhanova, Kulzhan ; Zhumadilov, Zhaxybay ; Imahori, Yoshio ; Itami, Jun ; Ono, Koji ; Masunaga, Shinichiro ; Masutani, Mitsuko. / Proteomic analysis of cellular response induced by boron neutron capture reaction in human squamous cell carcinoma SAS cells. In: Applied Radiation and Isotopes. 2015 ; Vol. 106. pp. 213-219.
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