Psalmotoxin-1 docking to human acid-sensing ion channel-1

Yawar J. Qadri, Bakhrom K. Berdiev, Yuhua Song, Howard L. Lippton, Catherine M. Fuller, Dale J. Benos

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Acid-sensing ion channel-1 (ASIC-1) is a proton-gated ion channel implicated in nociception and neuronal death during ischemia. Recently the first crystal structure of a chicken ASIC was obtained. Expanding upon this work, homology models of the human ASICs were constructed and evaluated. Energy-minimized structures were tested for validity by in silico docking of the models to psalmotoxin-1, which potently inhibits ASIC-1 and not other members of the family. The data are consistent with prior radioligand binding and functional assays while also explaining the selectivity of PcTX-1 for homomeric hASIC-1a. Binding energy calculations suggest that the toxin and channel create a complex that is more stable than the channel alone. The binding is dominated by the coulombic contributions, which account for why the toxin-channel interaction is not observed at low pH. The computational data were experimentally verified with single channel and whole-cell electrophysiological studies. These validated models should allow for the rational design of specific and potent peptidomimetic compounds that may be useful for the treatment of pain or ischemic stroke.

Original languageEnglish
Pages (from-to)17625-17633
Number of pages9
JournalJournal of Biological Chemistry
Volume284
Issue number26
DOIs
Publication statusPublished - Jun 26 2009
Externally publishedYes

Fingerprint

Acid Sensing Ion Channels
Application specific integrated circuits
Peptidomimetics
Nociception
Ion Channels
Computer Simulation
Protons
Chickens
Ischemia
Stroke
Binding energy
Pain
Assays
Crystal structure
Therapeutics

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Qadri, Y. J., Berdiev, B. K., Song, Y., Lippton, H. L., Fuller, C. M., & Benos, D. J. (2009). Psalmotoxin-1 docking to human acid-sensing ion channel-1. Journal of Biological Chemistry, 284(26), 17625-17633. https://doi.org/10.1074/jbc.M109.003913

Psalmotoxin-1 docking to human acid-sensing ion channel-1. / Qadri, Yawar J.; Berdiev, Bakhrom K.; Song, Yuhua; Lippton, Howard L.; Fuller, Catherine M.; Benos, Dale J.

In: Journal of Biological Chemistry, Vol. 284, No. 26, 26.06.2009, p. 17625-17633.

Research output: Contribution to journalArticle

Qadri, YJ, Berdiev, BK, Song, Y, Lippton, HL, Fuller, CM & Benos, DJ 2009, 'Psalmotoxin-1 docking to human acid-sensing ion channel-1', Journal of Biological Chemistry, vol. 284, no. 26, pp. 17625-17633. https://doi.org/10.1074/jbc.M109.003913
Qadri YJ, Berdiev BK, Song Y, Lippton HL, Fuller CM, Benos DJ. Psalmotoxin-1 docking to human acid-sensing ion channel-1. Journal of Biological Chemistry. 2009 Jun 26;284(26):17625-17633. https://doi.org/10.1074/jbc.M109.003913
Qadri, Yawar J. ; Berdiev, Bakhrom K. ; Song, Yuhua ; Lippton, Howard L. ; Fuller, Catherine M. ; Benos, Dale J. / Psalmotoxin-1 docking to human acid-sensing ion channel-1. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 26. pp. 17625-17633.
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