PTK7-Src Signaling at Epithelial Cell Contacts Mediates Spatial Organization of Actomyosin and Planar Cell Polarity

Anna Andreeva, Jianyi Lee, Madhura Lohia, Xiaoji Wu, Ian G. Macara, Xiaowei Lu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Actomyosin contractility plays a key role in tissue morphogenesis. During mammalian development, PTK7 regulates epithelial morphogenesis and planar cell polarity (PCP) through modulation of actomyosin contractility, but the underlying mechanism is unknown. Here, we show that PTK7 interacts with the tyrosine kinase Src and stimulates Src signaling along cell-cell contacts. We further identify ROCK2 as a target of junctional PTK7-Src signaling. PTK7 knockdown in cultured epithelial cells reduced the level of active Src at cell-cell contacts, resulting in delocalization of ROCK2 from cell-cell contacts and decreased junctional contractility, with a concomitant increase in actomyosin on the basal surface. Moreover, we present invivo evidence that Src family kinase (SFK) activity is critical for PCP regulation in the auditory sensory epithelium and that PTK7-SFK signaling regulates tyrosine phosphorylation of junctional ROCK2. Together, these results delineate a PTK7-Src signaling module for spatial regulation of ROCK activity, actomyosin contractility, and epithelial PCP.

Original languageEnglish
Pages (from-to)20-33
Number of pages14
JournalDevelopmental Cell
Volume29
Issue number1
DOIs
Publication statusPublished - Apr 14 2014
Externally publishedYes

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Actomyosin
Cell Polarity
src-Family Kinases
Epithelial Cells
Cell signaling
Morphogenesis
Phosphorylation
Tyrosine
Modulation
Tissue
Cultured Cells
Epithelium

ASJC Scopus subject areas

  • Developmental Biology

Cite this

PTK7-Src Signaling at Epithelial Cell Contacts Mediates Spatial Organization of Actomyosin and Planar Cell Polarity. / Andreeva, Anna; Lee, Jianyi; Lohia, Madhura; Wu, Xiaoji; Macara, Ian G.; Lu, Xiaowei.

In: Developmental Cell, Vol. 29, No. 1, 14.04.2014, p. 20-33.

Research output: Contribution to journalArticle

Andreeva, Anna ; Lee, Jianyi ; Lohia, Madhura ; Wu, Xiaoji ; Macara, Ian G. ; Lu, Xiaowei. / PTK7-Src Signaling at Epithelial Cell Contacts Mediates Spatial Organization of Actomyosin and Planar Cell Polarity. In: Developmental Cell. 2014 ; Vol. 29, No. 1. pp. 20-33.
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