Purification, structural and immunological characterization of a timothy grass (Phleum pratense) pollen allergen, Phl p 4, with cross-reactive potential

Sabina Stumvoll, Jonas Lidholm, Ronald Thunberg, Asa Marknell DeWitt, Petra Eibesteiner, Ines Swoboda, Agnes Bugajska-Schretter, Susanne Spitzauer, Luca Vangelista, Lili Kazemi-Shirazi, Wolfgang R. Sperr, Peter Valent, Dietrich Kraft, Rudolf Valenta

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Almost 500 million people worldwide suffer from Type I allergy, a genetically determined immunodisorder which is based on the production of IgE antibodies against per se harmless antigens (allergens). Due to their worldwide distribution and heavy pollen production, grasses represent a major allergen source for approximately 40% of allergic patients. We purified Phl p 4, a major timothy grass (Phleum pratense) pollen allergen with a molecular mass of 61.3 kDa and a pl of 9.6 to homogeneity. Circular dichroism spectroscopical analysis indicates that Phl p 4 contains a mixed α-helical/β-pleated secondary structure and, unlike many other allergens, showed no reversible unfolding after thermal denaturation. We show that Phl p 4 is a major allergen which reacts with IgE antibodies of 75% of grass pollen allergic patients (n=150) and induces basophil histamine release as well as immediate type skin reactions in sensitized individuals. Phl p 4-specific IgE from three patients as well as two rabbit-anti Phl p 4 antisera cross-reacted with allergens present in pollen of trees, grasses, weeds as well as plant-derived food. Rabbit antibodies raised against Phl p 4 also inhibited the binding of allergic patients IgE to Phl p 4. Phl p 4 may thus be used for diagnosis and treatment of sensitized allergic patients.

Original languageEnglish
Pages (from-to)1383-1396
Number of pages14
JournalBiological Chemistry
Volume383
Issue number9
DOIs
Publication statusPublished - Sep 1 2002

Keywords

  • Allergy
  • Circular dichroism spectroscopy
  • Grass pollen allergen
  • IgE cross-reactivity
  • Phl p 4

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Purification, structural and immunological characterization of a timothy grass (Phleum pratense) pollen allergen, Phl p 4, with cross-reactive potential'. Together they form a unique fingerprint.

  • Cite this

    Stumvoll, S., Lidholm, J., Thunberg, R., DeWitt, A. M., Eibesteiner, P., Swoboda, I., Bugajska-Schretter, A., Spitzauer, S., Vangelista, L., Kazemi-Shirazi, L., Sperr, W. R., Valent, P., Kraft, D., & Valenta, R. (2002). Purification, structural and immunological characterization of a timothy grass (Phleum pratense) pollen allergen, Phl p 4, with cross-reactive potential. Biological Chemistry, 383(9), 1383-1396. https://doi.org/10.1515/BC.2002.157