Quantitative analisys of movements of cytoplasmic granules in polarized fibroblasts

I. S. Grigoriev, A. A. Chernobelskaya, I. A. Vorobjev

Research output: Contribution to journalArticle

Abstract

Movements of cytoplasmic organelles were analyzed in Vero fibroblasts. In the cells polarized at the edge of an experimental wound, cytoplasmic granules moved randomly (Brownian movements) and by separate jumps (saltatory movements). The displacement of granules by the Brownian movements exceeded by more than an order of magnitude that mitochondria similar in weight. Lipid droplets moved predominantly by saltations, whereas mitochondria and lysosomes moved much less often. In a front part of the polarized cells, the main directions of saltatory movements were from a nucleus to a leading edge of a cell and back, whereas the tangential movements {across the long axis of a cell) comprised below 1%. 90% of saltatory movings occured in the area starting 10-12 μm from the nucleus and ending 10-12 μm from the leading edge of a cell. The average rate of saltatory movements of the granules (2.38 μm/s) were identical in both directions. The average length of the track was 7.49 μm; the maximum track lengh reached 30 μm. An increase in the granule diameter from 0.3 to 1.4 μm resulted in an insignificant (statistically insignificant) decrease in the movement average speed. The average speed of saltatory movements of mitochondria was 1.00 μm/s, and the average track lengh was 6,04 μm. Therefore, mitochondria, in contrast to lipid droplets, are rigidly fixed in the cytoplasm, and the force, holding mitochondria, is equal to the force produced by the microtubule-associated motors. Taking into account the characteristic of the centrifugal saltations, we suggested that thay are mediated by an unusual dynein.

Original languageEnglish
Pages (from-to)172-173
Number of pages2
JournalBiologicheskie Membrany
Volume14
Issue number2
Publication statusPublished - 1997
Externally publishedYes

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Cytoplasmic Granules
Mitochondria
Fibroblasts
Brownian movement
Lipids
Dyneins
Lysosomes
Microtubules
Organelles
Cytoplasm
Weights and Measures
Wounds and Injuries

ASJC Scopus subject areas

  • Biophysics
  • Clinical Biochemistry

Cite this

Quantitative analisys of movements of cytoplasmic granules in polarized fibroblasts. / Grigoriev, I. S.; Chernobelskaya, A. A.; Vorobjev, I. A.

In: Biologicheskie Membrany, Vol. 14, No. 2, 1997, p. 172-173.

Research output: Contribution to journalArticle

Grigoriev, I. S. ; Chernobelskaya, A. A. ; Vorobjev, I. A. / Quantitative analisys of movements of cytoplasmic granules in polarized fibroblasts. In: Biologicheskie Membrany. 1997 ; Vol. 14, No. 2. pp. 172-173.
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