Abstract
This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities’ distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 3-11 |
| Number of pages | 9 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 1389 |
| DOIs | |
| Publication status | Published - 2016 |
| Externally published | Yes |
Fingerprint
Keywords
- Autofluorescence
- Automated image analysis
- Flow cytometry
- Imaging flow cytometry
- Quantitative morphology
- Spectral compensation
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Quantitative functional morphology by imaging flow cytometry. / Vorobjev, Ivan A.; Barteneva, Natasha S.
In: Methods in molecular biology (Clifton, N.J.), Vol. 1389, 2016, p. 3-11.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative functional morphology by imaging flow cytometry
AU - Vorobjev, Ivan A.
AU - Barteneva, Natasha S.
PY - 2016
Y1 - 2016
N2 - This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities’ distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.
AB - This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities’ distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.
KW - Autofluorescence
KW - Automated image analysis
KW - Flow cytometry
KW - Imaging flow cytometry
KW - Quantitative morphology
KW - Spectral compensation
UR - http://www.scopus.com/inward/record.url?scp=84990026596&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990026596&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3302-0_1
DO - 10.1007/978-1-4939-3302-0_1
M3 - Article
VL - 1389
SP - 3
EP - 11
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -