Quantitative functional morphology by imaging flow cytometry

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Abstract

This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities’ distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.

Original languageEnglish
Pages (from-to)3-11
Number of pages9
JournalMethods in molecular biology (Clifton, N.J.)
Volume1389
DOIs
Publication statusPublished - 2016
Externally publishedYes

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Keywords

  • Autofluorescence
  • Automated image analysis
  • Flow cytometry
  • Imaging flow cytometry
  • Quantitative morphology
  • Spectral compensation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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