Quantitative functional morphology by imaging flow cytometry

Ivan A. Vorobjev; Natasha S. Barteneva

doi:10.1007/978-1-4939-3302-0_1

Quantitative functional morphology by imaging flow cytometry

Ivan A. Vorobjev, Natasha S. Barteneva

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities’ distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.

Original language	English
Pages (from-to)	3-11
Number of pages	9
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1389
DOIs	https://doi.org/10.1007/978-1-4939-3302-0_1
Publication status	Published - 2016
Externally published	Yes

Keywords

Autofluorescence
Automated image analysis
Flow cytometry
Imaging flow cytometry
Quantitative morphology
Spectral compensation

ASJC Scopus subject areas

Molecular Biology
Genetics

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abstract = "This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities{\textquoteright} distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.",

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KW - Autofluorescence

KW - Automated image analysis

KW - Flow cytometry

KW - Imaging flow cytometry

KW - Quantitative morphology

KW - Spectral compensation

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