TY - JOUR
T1 - Quantitative functional morphology by imaging flow cytometry
AU - Vorobjev, Ivan A.
AU - Barteneva, Natasha S.
N1 - Funding Information:
The work was supported in part by NIH S10 RR023459 grant, Harvard Pilot grant, and the Russian Foundation for Basic Research grants 13-04-40189-H and 11-04-01749a. We are also grateful to Aleksandra Gorelova (Harvard University) for help with the editing and preparation of the manuscript.
Publisher Copyright:
© Springer Science+Business Media New York 2016.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016
Y1 - 2016
N2 - This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities’ distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.
AB - This chapter describes advantages and limitations of imaging flow cytometry (IFC) based on Imagestream instrumentation using a hybrid approach of morphometric measurement and quantitation of multiparametric fluorescent intensities’ distribution in cells and particles. Brief comparison is given of IFC with conventional flow cytometry and fluorescent microscopy. Some future directions of the IFC technology are described and discussed.
KW - Autofluorescence
KW - Automated image analysis
KW - Flow cytometry
KW - Imaging flow cytometry
KW - Quantitative morphology
KW - Spectral compensation
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U2 - 10.1007/978-1-4939-3302-0_1
DO - 10.1007/978-1-4939-3302-0_1
M3 - Article
AN - SCOPUS:84990026596
VL - 1389
SP - 3
EP - 11
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -