TY - JOUR
T1 - Rapid detection of carcinoembryonic antigen by means of an electrochemical aptasensor
AU - Yunussova, Nigara
AU - Tilegen, Meruyert
AU - Pham, Tri Thanh
AU - Kanayeva, Damira
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/5/17
Y1 - 2024/5/17
N2 - Carcinoembryonic antigen (CEA) is a critical biomarker for identifying colon cancer. This work presents an electrochemical impedance spectroscopy (EIS) based aptasensor for detecting CEA, utilizing a single-stranded DNA (ssDNA) aptamer previously selected and characterized by our research group. The surface of an interdigitated gold electrode (IDE) was successfully functionalized with an 18-HEG-modified aptamer sequence. The developed aptasensor demonstrated high specificity and sensitivity with detection limits of 2.4 pg/mL and 3.8 pg/mL for CEA in buffer and human serum samples, respectively. The optimal incubation time for the target protein was 20 min, and EIS measurements took less than 3 min. Atomic force microscopy (AFM) micrographs supported the EIS data, demonstrating a change in IDE surface roughness after each modification step, confirming the successful capture of the target. The potential of this developed EIS aptasensor in detecting CEA in complex samples holds promise.
AB - Carcinoembryonic antigen (CEA) is a critical biomarker for identifying colon cancer. This work presents an electrochemical impedance spectroscopy (EIS) based aptasensor for detecting CEA, utilizing a single-stranded DNA (ssDNA) aptamer previously selected and characterized by our research group. The surface of an interdigitated gold electrode (IDE) was successfully functionalized with an 18-HEG-modified aptamer sequence. The developed aptasensor demonstrated high specificity and sensitivity with detection limits of 2.4 pg/mL and 3.8 pg/mL for CEA in buffer and human serum samples, respectively. The optimal incubation time for the target protein was 20 min, and EIS measurements took less than 3 min. Atomic force microscopy (AFM) micrographs supported the EIS data, demonstrating a change in IDE surface roughness after each modification step, confirming the successful capture of the target. The potential of this developed EIS aptasensor in detecting CEA in complex samples holds promise.
KW - Applied sciences
KW - Bio-electrochemistry
KW - Sensor system
UR - http://www.scopus.com/inward/record.url?scp=85190345177&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85190345177&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2024.109637
DO - 10.1016/j.isci.2024.109637
M3 - Article
AN - SCOPUS:85190345177
SN - 2589-0042
VL - 27
JO - iScience
JF - iScience
IS - 5
M1 - 109637
ER -
