Рекомбинантная тирозиназа Verrucomicrobium spinosum: выделение, характеристика и использование для производства белка с адгезивными свойствами

Translated title of the contribution: Recombinant tyrosinase from verrucomicrobium spinosum: Isolation, characteristics and use for production of protein with adhesive properties

A. S. Aksambayeva, L. R. Zhaparova, Zh S. Shagyrova, E. Zhiyenbay, Talgat Nurgozhin, E. M. Ramankulov, A. V. Shustov

Research output: Contribution to journalArticle

Abstract

The recombinant tyrosinase from the Verrucomicrobium spinosum bacterium has been obtained using the heterological expression in E. coli and isolated in the pure state to determine a number of its biochemical properties (kinetics of the enzymatic reaction, effect of an inhibitor and activator, and pH optimum). Co-expression of the genes for tyrosinase and recombinant adhesive mussel protein in one producer strain was used to introduce post-translational modifications into the adhesive protein. These modifications included the tyrosine transformation into DOPA residues which gives the protein an ability of molecular adhesion in aqueous medium. The resulting adhesive protein showed unexpectedly high adhesion strength of 2.5 MPa, which exceeds the corresponding values for natural adhesive proteins and is by a few tens times superior to the existing medical fibrin-based medical glues.

Original languageRussian
Pages (from-to)12-27
Number of pages16
JournalBiotekhnologiya
Volume33
Issue number6
DOIs
Publication statusPublished - Jan 1 2017

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Keywords

  • Adhesion strength
  • Adhesive protein
  • Biocompatible glue
  • DOPA
  • Enzymatic activity
  • Tyrosinase
  • Адгезивный белок
  • Адгезионная прочность
  • Биосовместимый клей
  • ДОФА
  • Тирозиназа
  • Ферментативная активность

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Ecology

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