Recombinant Tyrosinase from Verrucomicrobium spinosum: Isolation, Characteristics, and Use for the Production of a Protein with Adhesive Properties

A. S. Aksambayeva; L. R. Zhaparova; Zh S. Shagyrova; E. Zhiyenbay; Talgat Nurgozhin; E. M. Ramankulov; A. V. Shustov

doi:10.1134/S0003683818080021

Recombinant Tyrosinase from Verrucomicrobium spinosum: Isolation, Characteristics, and Use for the Production of a Protein with Adhesive Properties

A. S. Aksambayeva, L. R. Zhaparova, Zh S. Shagyrova, E. Zhiyenbay, Talgat Nurgozhin, E. M. Ramankulov, A. V. Shustov

Department of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The recombinant tyrosinase from Verrucomicrobium spinosum bacteria has been obtained by heterologous expression in E. coli and isolated in the pure state to determine some of its biochemical properties (the kinetics of the enzymatic reaction, the effect of an inhibitor and activator, and the pH optimum). Coexpression of the genes for tyrosinase and recombinant adhesive mussel protein in one producer strain was used to introduce posttranslational modifications into the adhesive protein. These modifications involved the transformation of tyrosine residues into DOPA residues, which makes the protein capable of molecular adhesion in an aqueous medium.

Original language	English
Pages (from-to)	780-792
Number of pages	13
Journal	Applied Biochemistry and Microbiology
Volume	54
Issue number	8
DOIs	https://doi.org/10.1134/S0003683818080021
Publication status	Published - Dec 1 2018

Keywords

DOPA
adhesion strength
adhesive protein
biocompatible glue
enzymatic activity
tyrosinase

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

Access to Document

10.1134/S0003683818080021

Cite this

Aksambayeva, A. S., Zhaparova, L. R., Shagyrova, Z. S., Zhiyenbay, E., Nurgozhin, T., Ramankulov, E. M., & Shustov, A. V. (2018). Recombinant Tyrosinase from Verrucomicrobium spinosum: Isolation, Characteristics, and Use for the Production of a Protein with Adhesive Properties. Applied Biochemistry and Microbiology, 54(8), 780-792. https://doi.org/10.1134/S0003683818080021

Recombinant Tyrosinase from Verrucomicrobium spinosum : Isolation, Characteristics, and Use for the Production of a Protein with Adhesive Properties. / Aksambayeva, A. S.; Zhaparova, L. R.; Shagyrova, Zh S.; Zhiyenbay, E.; Nurgozhin, Talgat; Ramankulov, E. M.; Shustov, A. V.

In: Applied Biochemistry and Microbiology, Vol. 54, No. 8, 01.12.2018, p. 780-792.

Research output: Contribution to journal › Article › peer-review

Aksambayeva, AS, Zhaparova, LR, Shagyrova, ZS, Zhiyenbay, E, Nurgozhin, T, Ramankulov, EM & Shustov, AV 2018, 'Recombinant Tyrosinase from Verrucomicrobium spinosum: Isolation, Characteristics, and Use for the Production of a Protein with Adhesive Properties', Applied Biochemistry and Microbiology, vol. 54, no. 8, pp. 780-792. https://doi.org/10.1134/S0003683818080021

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abstract = "The recombinant tyrosinase from Verrucomicrobium spinosum bacteria has been obtained by heterologous expression in E. coli and isolated in the pure state to determine some of its biochemical properties (the kinetics of the enzymatic reaction, the effect of an inhibitor and activator, and the pH optimum). Coexpression of the genes for tyrosinase and recombinant adhesive mussel protein in one producer strain was used to introduce posttranslational modifications into the adhesive protein. These modifications involved the transformation of tyrosine residues into DOPA residues, which makes the protein capable of molecular adhesion in an aqueous medium.",

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note = "Funding Information: ACKNOWLEDGMENTS The work was supported by a grant from the Ministry of Education and Science of the Republic of Kazakhstan (no. 0115PK01798, “Bioadhesive Microparticles Releasing Antimicrobial Peptides, a New Preparation for Prophylaxis and Therapy of Caries”). Publisher Copyright: {\textcopyright} 2018, Pleiades Publishing, Inc. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

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AU - Nurgozhin, Talgat

AU - Ramankulov, E. M.

AU - Shustov, A. V.

N1 - Funding Information: ACKNOWLEDGMENTS The work was supported by a grant from the Ministry of Education and Science of the Republic of Kazakhstan (no. 0115PK01798, “Bioadhesive Microparticles Releasing Antimicrobial Peptides, a New Preparation for Prophylaxis and Therapy of Caries”). Publisher Copyright: © 2018, Pleiades Publishing, Inc. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

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Recombinant Tyrosinase from Verrucomicrobium spinosum: Isolation, Characteristics, and Use for the Production of a Protein with Adhesive Properties

Abstract

Keywords

ASJC Scopus subject areas

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