Recombinant Tyrosinase from Verrucomicrobium spinosum

Isolation, Characteristics, and Use for the Production of a Protein with Adhesive Properties

A. S. Aksambayeva, L. R. Zhaparova, Zh S. Shagyrova, E. Zhiyenbay, Talgat Nurgozhin, E. M. Ramankulov, A. V. Shustov

Research output: Contribution to journalArticle

Abstract

The recombinant tyrosinase from Verrucomicrobium spinosum bacteria has been obtained by heterologous expression in E. coli and isolated in the pure state to determine some of its biochemical properties (the kinetics of the enzymatic reaction, the effect of an inhibitor and activator, and the pH optimum). Coexpression of the genes for tyrosinase and recombinant adhesive mussel protein in one producer strain was used to introduce posttranslational modifications into the adhesive protein. These modifications involved the transformation of tyrosine residues into DOPA residues, which makes the protein capable of molecular adhesion in an aqueous medium.

Original languageEnglish
Pages (from-to)780-792
Number of pages13
JournalApplied Biochemistry and Microbiology
Volume54
Issue number8
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Verrucomicrobia
Monophenol Monooxygenase
Post Translational Protein Processing
Adhesives
Escherichia coli
Tyrosine
Bacteria
Proteins
Adhesion
Genes
Kinetics

Keywords

  • adhesion strength
  • adhesive protein
  • biocompatible glue
  • DOPA
  • enzymatic activity
  • tyrosinase

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Recombinant Tyrosinase from Verrucomicrobium spinosum : Isolation, Characteristics, and Use for the Production of a Protein with Adhesive Properties. / Aksambayeva, A. S.; Zhaparova, L. R.; Shagyrova, Zh S.; Zhiyenbay, E.; Nurgozhin, Talgat; Ramankulov, E. M.; Shustov, A. V.

In: Applied Biochemistry and Microbiology, Vol. 54, No. 8, 01.12.2018, p. 780-792.

Research output: Contribution to journalArticle

Aksambayeva, A. S. ; Zhaparova, L. R. ; Shagyrova, Zh S. ; Zhiyenbay, E. ; Nurgozhin, Talgat ; Ramankulov, E. M. ; Shustov, A. V. / Recombinant Tyrosinase from Verrucomicrobium spinosum : Isolation, Characteristics, and Use for the Production of a Protein with Adhesive Properties. In: Applied Biochemistry and Microbiology. 2018 ; Vol. 54, No. 8. pp. 780-792.
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