Regulation of epithelial sodium channels by short actin filaments

Bakhram K. Berdiev, Adriana G. Prat, Horacio F. Cantiello, Dennis A. Ausiello, Catherine M. Fuller, Biljana Jovov, Dale J. Benos, Iskander I. Ismailov

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Abstract

Cytoskeletal elements play an important role in the regulation of ion transport in epithelia. We have studied the effects of actin filaments of different length on the α, β, γ-rENaC (rat epithelial Na+ channel) in planar lipid bilayers. We found the following. 1) Short actin filaments caused a 2-fold decrease in unitary conductance and a 2-fold increase in open probability (P(o)) of α,β,γ-rENaC. 2) α,β,γ-rENaC could be transiently activated by protein kinase A (PKA) plus ATP in the presence, but not in the absence, of actin. 3) ATP in the presence of actin was also able to induce a transitory activation of α,β,γ,-rENaC, although with a shortened time course and with a lower magnitude of change in P(o). 4) DNase I, an agent known to prohibit elongation of actin filaments, prevented activation of α,β,γ-rENaC by ATP or PKA plus ATP. 5) Cytochalasin D, added after rundown of α,β,γ-rENaC activity following ATP or PKA plus ATP treatment, produced a second transient activation of α,β,γ-rENaC. 6) Gelsolin, a protein that stabilizes polymerization of actin filaments at certain lengths, evoked a sustained activation of α,β,γ-rENaC at actin/gelsolin ratios of + channels and that interaction of these channels with other associated proteins, such as short actin filaments, confers regulation to channel activity.

Original languageEnglish
Pages (from-to)17704-17710
Number of pages7
JournalJournal of Biological Chemistry
Volume271
Issue number30
DOIs
Publication statusPublished - 1996
Externally publishedYes

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Epithelial Sodium Channels
Actin Cytoskeleton
Actins
Adenosine Triphosphate
Cyclic AMP-Dependent Protein Kinases
Gelsolin
Chemical activation
Cytochalasin D
Deoxyribonuclease I
Ion Transport
Lipid Bilayers
Polymerization
Lipid bilayers
Epithelium
Rats
Elongation
Proteins
Ions

ASJC Scopus subject areas

  • Biochemistry

Cite this

Berdiev, B. K., Prat, A. G., Cantiello, H. F., Ausiello, D. A., Fuller, C. M., Jovov, B., ... Ismailov, I. I. (1996). Regulation of epithelial sodium channels by short actin filaments. Journal of Biological Chemistry, 271(30), 17704-17710. https://doi.org/10.1074/jbc.271.30.17704

Regulation of epithelial sodium channels by short actin filaments. / Berdiev, Bakhram K.; Prat, Adriana G.; Cantiello, Horacio F.; Ausiello, Dennis A.; Fuller, Catherine M.; Jovov, Biljana; Benos, Dale J.; Ismailov, Iskander I.

In: Journal of Biological Chemistry, Vol. 271, No. 30, 1996, p. 17704-17710.

Research output: Contribution to journalArticle

Berdiev, BK, Prat, AG, Cantiello, HF, Ausiello, DA, Fuller, CM, Jovov, B, Benos, DJ & Ismailov, II 1996, 'Regulation of epithelial sodium channels by short actin filaments', Journal of Biological Chemistry, vol. 271, no. 30, pp. 17704-17710. https://doi.org/10.1074/jbc.271.30.17704
Berdiev BK, Prat AG, Cantiello HF, Ausiello DA, Fuller CM, Jovov B et al. Regulation of epithelial sodium channels by short actin filaments. Journal of Biological Chemistry. 1996;271(30):17704-17710. https://doi.org/10.1074/jbc.271.30.17704
Berdiev, Bakhram K. ; Prat, Adriana G. ; Cantiello, Horacio F. ; Ausiello, Dennis A. ; Fuller, Catherine M. ; Jovov, Biljana ; Benos, Dale J. ; Ismailov, Iskander I. / Regulation of epithelial sodium channels by short actin filaments. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 30. pp. 17704-17710.
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AU - Cantiello, Horacio F.

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AU - Fuller, Catherine M.

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