Regulation of epithelial sodium channels by the cystic fibrosis transmembrane conductance regulator

Iskander L. Ismailov, Mouhamed S. Awayda, Biljana Jovov, Bakhram K. Berdiev, Catherine M. Fuller, John R. Dedman, Marcia A. Kaetzel, Dale J. Benos

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Cystic fibrosis airway epithelia exhibit enhanced Na+ reabsorption in parallel with diminished Cl- secretion. We tested the hypothesis that the cystic fibrosis transmembrane conductance regulator (CFTR) directly affects epithelial Na+ channel activity by co-incorporating into planar lipid bilayers immunopurified bovine tracheal CFTR and either heterologously expressed rat epithelial Na+ channel (α,β,γ-rENaC) or an immunopurified bovine renal Na+ channel protein complex. The single channel open probability (P(o)) of rENaC was decreased by 24% in the presence of CFTR. Protein kinase A (PKA) plus ATP activated CFTR, but did not have any effect on rENaC. CFTR also decreased the extent of elevation of the renal Na+ channel P(o) following PKA-mediated phosphorylation. Moreover, the presence of CFTR prohibited the inward rectification of the gating of this renal Na+ channel normally induced by PKA-mediated phosphorylation, thus down- regulating inward Na+ current. This interaction between CFTR and Na+ channels occurs independently of whether or not wild-type CFTR is conducting anions. However, the nonconductive CFTR mutant, G551D CFTR, cannot substitute for the wild-type molecule. Our results indicate that CFTR can directly down- regulate single Na+ channel activity, thus accounting, at least in part, for the observed differences in Na+ transport between normal and cystic fibrosis-affected airway epithelia.

Original languageEnglish
Pages (from-to)4725-4732
Number of pages8
JournalJournal of Biological Chemistry
Volume271
Issue number9
DOIs
Publication statusPublished - Mar 1 1996
Externally publishedYes

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Epithelial Sodium Channels
Cystic Fibrosis Transmembrane Conductance Regulator
Cyclic AMP-Dependent Protein Kinases
Phosphorylation
Kidney
Cystic Fibrosis
Epithelium
Lipid bilayers
Lipid Bilayers
Anions
Rats
Down-Regulation
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ismailov, I. L., Awayda, M. S., Jovov, B., Berdiev, B. K., Fuller, C. M., Dedman, J. R., ... Benos, D. J. (1996). Regulation of epithelial sodium channels by the cystic fibrosis transmembrane conductance regulator. Journal of Biological Chemistry, 271(9), 4725-4732. https://doi.org/10.1074/jbc.271.9.4725

Regulation of epithelial sodium channels by the cystic fibrosis transmembrane conductance regulator. / Ismailov, Iskander L.; Awayda, Mouhamed S.; Jovov, Biljana; Berdiev, Bakhram K.; Fuller, Catherine M.; Dedman, John R.; Kaetzel, Marcia A.; Benos, Dale J.

In: Journal of Biological Chemistry, Vol. 271, No. 9, 01.03.1996, p. 4725-4732.

Research output: Contribution to journalArticle

Ismailov, IL, Awayda, MS, Jovov, B, Berdiev, BK, Fuller, CM, Dedman, JR, Kaetzel, MA & Benos, DJ 1996, 'Regulation of epithelial sodium channels by the cystic fibrosis transmembrane conductance regulator', Journal of Biological Chemistry, vol. 271, no. 9, pp. 4725-4732. https://doi.org/10.1074/jbc.271.9.4725
Ismailov, Iskander L. ; Awayda, Mouhamed S. ; Jovov, Biljana ; Berdiev, Bakhram K. ; Fuller, Catherine M. ; Dedman, John R. ; Kaetzel, Marcia A. ; Benos, Dale J. / Regulation of epithelial sodium channels by the cystic fibrosis transmembrane conductance regulator. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 9. pp. 4725-4732.
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