Reliability of immunohistochemical demonstration of oestrogen receptors in routine practice

Interlaboratory variance in the sensitivity of detection and evaluation of scoring systems

A. Rhodes, B. Jasani, D. M. Barnes, L. G. Bobrow, K. D. Miller

Research output: Contribution to journalArticle

280 Citations (Scopus)

Abstract

Aims - To investigate interlaboratory variance in the immunohistochemical (IHC) detection of oestrogen receptors so as to determine the rate of false negatives, which could adversely influence the decision to give adjuvant tamoxifen treatment. Methods - To ensure that similar results are obtained by different institutions, 200 laboratories from 26 countries have joined the UK national external quality assessment scheme for immunocytochemistry (NEQAS-ICC). Histological sections from breast cancers having low, medium, and high levels of oestrogen receptor expression were sent to each of the laboratories for immunohistochemical staining. The results obtained were evaluated for the sensitivity of detection, first by estimating threshold values of 1% and 10% of stained tumour cells, and second by the Quick score method, by a panel of four assessors judging individual sections independently on a single blind basis. The results were also evaluated using participants' own threshold values. Results - Over 80% of laboratories were able to demonstrate oestrogen receptor positivity on the medium and high expressing tumours, but only 37% of laboratories scored adequately on the low expressing tumour. Approximately one third of laboratories failed to register any positive staining in this tumour, while one third showed only minimal positivity. Conclusions - There is considerable interlaboratory variability, especially in relation to the detection of breast cancers with low oestrogen receptor positivity, with a false negative rate of between 30% and 60%. This variability appears to be caused by minor differences in methodology that may be rectified by fine adjustment of overall technique.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalJournal of Clinical Pathology
Volume53
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Estrogen Receptors
Neoplasms
Staining and Labeling
Breast Neoplasms
Tamoxifen
Immunohistochemistry
Therapeutics

Keywords

  • Immunohistochemistry
  • Interlaboratory variation
  • Oestrogen receptors

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Reliability of immunohistochemical demonstration of oestrogen receptors in routine practice : Interlaboratory variance in the sensitivity of detection and evaluation of scoring systems. / Rhodes, A.; Jasani, B.; Barnes, D. M.; Bobrow, L. G.; Miller, K. D.

In: Journal of Clinical Pathology, Vol. 53, No. 2, 2000, p. 125-130.

Research output: Contribution to journalArticle

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abstract = "Aims - To investigate interlaboratory variance in the immunohistochemical (IHC) detection of oestrogen receptors so as to determine the rate of false negatives, which could adversely influence the decision to give adjuvant tamoxifen treatment. Methods - To ensure that similar results are obtained by different institutions, 200 laboratories from 26 countries have joined the UK national external quality assessment scheme for immunocytochemistry (NEQAS-ICC). Histological sections from breast cancers having low, medium, and high levels of oestrogen receptor expression were sent to each of the laboratories for immunohistochemical staining. The results obtained were evaluated for the sensitivity of detection, first by estimating threshold values of 1{\%} and 10{\%} of stained tumour cells, and second by the Quick score method, by a panel of four assessors judging individual sections independently on a single blind basis. The results were also evaluated using participants' own threshold values. Results - Over 80{\%} of laboratories were able to demonstrate oestrogen receptor positivity on the medium and high expressing tumours, but only 37{\%} of laboratories scored adequately on the low expressing tumour. Approximately one third of laboratories failed to register any positive staining in this tumour, while one third showed only minimal positivity. Conclusions - There is considerable interlaboratory variability, especially in relation to the detection of breast cancers with low oestrogen receptor positivity, with a false negative rate of between 30{\%} and 60{\%}. This variability appears to be caused by minor differences in methodology that may be rectified by fine adjustment of overall technique.",
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AU - Miller, K. D.

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AB - Aims - To investigate interlaboratory variance in the immunohistochemical (IHC) detection of oestrogen receptors so as to determine the rate of false negatives, which could adversely influence the decision to give adjuvant tamoxifen treatment. Methods - To ensure that similar results are obtained by different institutions, 200 laboratories from 26 countries have joined the UK national external quality assessment scheme for immunocytochemistry (NEQAS-ICC). Histological sections from breast cancers having low, medium, and high levels of oestrogen receptor expression were sent to each of the laboratories for immunohistochemical staining. The results obtained were evaluated for the sensitivity of detection, first by estimating threshold values of 1% and 10% of stained tumour cells, and second by the Quick score method, by a panel of four assessors judging individual sections independently on a single blind basis. The results were also evaluated using participants' own threshold values. Results - Over 80% of laboratories were able to demonstrate oestrogen receptor positivity on the medium and high expressing tumours, but only 37% of laboratories scored adequately on the low expressing tumour. Approximately one third of laboratories failed to register any positive staining in this tumour, while one third showed only minimal positivity. Conclusions - There is considerable interlaboratory variability, especially in relation to the detection of breast cancers with low oestrogen receptor positivity, with a false negative rate of between 30% and 60%. This variability appears to be caused by minor differences in methodology that may be rectified by fine adjustment of overall technique.

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