Reme1, a Copia retrotransposon in melon, is transcriptionally induced by UV light

Elisenda Ramallo, Ruslan Kalendar, Alan H. Schulman, José A. Martínez-Izquierdo

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

For the first time, numerous sequences of Copia and Gypsy retrotransposons from the Cucumis melo genome have been obtained and analyzed. Phylogenetic analyses of sequences of both types of long terminal repeat (LTR) retrotransposons were carried out. The melon genome contains approximately 20,000 Gypsy and 6,800 Copia elements, comprising about 26% of its total size. Starting from a retrotransposon fragment, we have cloned and characterized an entire melon retrotransposon, named Reme1, which is 5,149 bp long. Reme1 belongs to the Superfamily Copia retrotransposons by its protein domain order and sequence similarity to other Copia elements of dicotyledons. The haploid genome of melon (var. "Piel de Sapo") contains about 120 copies of Reme1. Several copies of Reme1 are transcriptionally active, although at low levels, in melon leaves as analyzed by reverse-transcription PCR (RT-PCR) and sequencing. However, the transcript pool is considerably increased when melon leaves are treated with UV light, as has been seen for various retroelements in many organisms. The cDNAs of Reme1 transcripts showed less diversity than do Reme1 genomic sequences, suggesting that a subfamily of these elements is differentially responsive to UV.

Original languageEnglish
Pages (from-to)137-150
Number of pages14
JournalPlant Molecular Biology
Volume66
Issue number1-2
DOIs
Publication statusPublished - Jan 2008
Externally publishedYes

Keywords

  • Copia
  • Cucumis melo
  • Genome dynamics
  • LTR retrotransposon
  • Stress-induced transcription
  • UV light

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'Reme1, a Copia retrotransposon in melon, is transcriptionally induced by UV light'. Together they form a unique fingerprint.

Cite this