Abstract
Members of the fibroblast growth factor (FGF) gene family control formation of the body plan and organogenesis in vertebrates. FGF3 is expressed in the developing hindbrain and has been shown to be involved in inner ear development of different vertebrate species, including zebrafish, Xenopus, chick and mouse. In the mouse, insertion of a neomycin resistance gene into the Fgf3 gene via homologous recombination results in severe developmental defects during differentiation of the otic vesicle. We have addressed the precise roles of FGF3 and other FGF family members during formation of the murine inner ear using both loss- and gain-of-function experiments. We generated a new mutant allele lacking the entire FGF3-coding region but surprisingly found no evidence for severe defects either during inner ear development or in the mature sensory organ, suggesting the functional involvement of other FGF family members during its formation. Ectopic expression of FGF10 in the developing hindbrain of transgenic mice leads to the formation of ectopic vesicles, expressing some otic marker genes and thus indicating a role for FGF10 during otic vesicle formation. Expression analysis of FGF10 during mouse embryogenesis reveals a highly dynamic pattern of expression in the developing hindbrain, partially overlapping with FGF3 expression and coinciding with formation of the inner ear. However, FGF10 mutant mice have been reported to display only mild defects during inner ear differentiation. We thus created double mutant mice for FGF3 and FGF10, which form severely reduced otic vesicles, suggesting redundant roles of these FGFs, acting in combination as neural signals for otic vesicle formation.
| Original language | English |
|---|---|
| Pages (from-to) | 6329-6338 |
| Number of pages | 10 |
| Journal | Development (Cambridge) |
| Volume | 130 |
| Issue number | 25 |
| DOIs | |
| Publication status | Published - Dec 2003 |
| Externally published | Yes |
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Keywords
- Fibroblast growth factor
- Hindbrain
- Mouse
- Otic vesicle
ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
Requirements for FGF3 and FGF10 during inner ear formation. / Alvarez, Yolanda; Alonso, Maria Teresa; Vendrell, Victor; Zelarayan, Laura Cecilia; Chamero, Pablo; Theil, Thomas; Bösl, Michael R.; Kato, Shigeaki; Maconochie, Mark; Rietmacher, Dieter; Schimmang, Thomas.
In: Development (Cambridge), Vol. 130, No. 25, 12.2003, p. 6329-6338.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Requirements for FGF3 and FGF10 during inner ear formation
AU - Alvarez, Yolanda
AU - Alonso, Maria Teresa
AU - Vendrell, Victor
AU - Zelarayan, Laura Cecilia
AU - Chamero, Pablo
AU - Theil, Thomas
AU - Bösl, Michael R.
AU - Kato, Shigeaki
AU - Maconochie, Mark
AU - Rietmacher, Dieter
AU - Schimmang, Thomas
PY - 2003/12
Y1 - 2003/12
N2 - Members of the fibroblast growth factor (FGF) gene family control formation of the body plan and organogenesis in vertebrates. FGF3 is expressed in the developing hindbrain and has been shown to be involved in inner ear development of different vertebrate species, including zebrafish, Xenopus, chick and mouse. In the mouse, insertion of a neomycin resistance gene into the Fgf3 gene via homologous recombination results in severe developmental defects during differentiation of the otic vesicle. We have addressed the precise roles of FGF3 and other FGF family members during formation of the murine inner ear using both loss- and gain-of-function experiments. We generated a new mutant allele lacking the entire FGF3-coding region but surprisingly found no evidence for severe defects either during inner ear development or in the mature sensory organ, suggesting the functional involvement of other FGF family members during its formation. Ectopic expression of FGF10 in the developing hindbrain of transgenic mice leads to the formation of ectopic vesicles, expressing some otic marker genes and thus indicating a role for FGF10 during otic vesicle formation. Expression analysis of FGF10 during mouse embryogenesis reveals a highly dynamic pattern of expression in the developing hindbrain, partially overlapping with FGF3 expression and coinciding with formation of the inner ear. However, FGF10 mutant mice have been reported to display only mild defects during inner ear differentiation. We thus created double mutant mice for FGF3 and FGF10, which form severely reduced otic vesicles, suggesting redundant roles of these FGFs, acting in combination as neural signals for otic vesicle formation.
AB - Members of the fibroblast growth factor (FGF) gene family control formation of the body plan and organogenesis in vertebrates. FGF3 is expressed in the developing hindbrain and has been shown to be involved in inner ear development of different vertebrate species, including zebrafish, Xenopus, chick and mouse. In the mouse, insertion of a neomycin resistance gene into the Fgf3 gene via homologous recombination results in severe developmental defects during differentiation of the otic vesicle. We have addressed the precise roles of FGF3 and other FGF family members during formation of the murine inner ear using both loss- and gain-of-function experiments. We generated a new mutant allele lacking the entire FGF3-coding region but surprisingly found no evidence for severe defects either during inner ear development or in the mature sensory organ, suggesting the functional involvement of other FGF family members during its formation. Ectopic expression of FGF10 in the developing hindbrain of transgenic mice leads to the formation of ectopic vesicles, expressing some otic marker genes and thus indicating a role for FGF10 during otic vesicle formation. Expression analysis of FGF10 during mouse embryogenesis reveals a highly dynamic pattern of expression in the developing hindbrain, partially overlapping with FGF3 expression and coinciding with formation of the inner ear. However, FGF10 mutant mice have been reported to display only mild defects during inner ear differentiation. We thus created double mutant mice for FGF3 and FGF10, which form severely reduced otic vesicles, suggesting redundant roles of these FGFs, acting in combination as neural signals for otic vesicle formation.
KW - Fibroblast growth factor
KW - Hindbrain
KW - Mouse
KW - Otic vesicle
UR - http://www.scopus.com/inward/record.url?scp=10744220359&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10744220359&partnerID=8YFLogxK
U2 - 10.1242/dev.00881
DO - 10.1242/dev.00881
M3 - Article
VL - 130
SP - 6329
EP - 6338
JO - Development
JF - Development
SN - 0950-1991
IS - 25
ER -