Requirements for FGF3 and FGF10 during inner ear formation

Yolanda Alvarez, Maria Teresa Alonso, Victor Vendrell, Laura Cecilia Zelarayan, Pablo Chamero, Thomas Theil, Michael R. Bösl, Shigeaki Kato, Mark Maconochie, Dieter Rietmacher, Thomas Schimmang

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Members of the fibroblast growth factor (FGF) gene family control formation of the body plan and organogenesis in vertebrates. FGF3 is expressed in the developing hindbrain and has been shown to be involved in inner ear development of different vertebrate species, including zebrafish, Xenopus, chick and mouse. In the mouse, insertion of a neomycin resistance gene into the Fgf3 gene via homologous recombination results in severe developmental defects during differentiation of the otic vesicle. We have addressed the precise roles of FGF3 and other FGF family members during formation of the murine inner ear using both loss- and gain-of-function experiments. We generated a new mutant allele lacking the entire FGF3-coding region but surprisingly found no evidence for severe defects either during inner ear development or in the mature sensory organ, suggesting the functional involvement of other FGF family members during its formation. Ectopic expression of FGF10 in the developing hindbrain of transgenic mice leads to the formation of ectopic vesicles, expressing some otic marker genes and thus indicating a role for FGF10 during otic vesicle formation. Expression analysis of FGF10 during mouse embryogenesis reveals a highly dynamic pattern of expression in the developing hindbrain, partially overlapping with FGF3 expression and coinciding with formation of the inner ear. However, FGF10 mutant mice have been reported to display only mild defects during inner ear differentiation. We thus created double mutant mice for FGF3 and FGF10, which form severely reduced otic vesicles, suggesting redundant roles of these FGFs, acting in combination as neural signals for otic vesicle formation.

Original languageEnglish
Pages (from-to)6329-6338
Number of pages10
JournalDevelopment (Cambridge)
Volume130
Issue number25
DOIs
Publication statusPublished - Dec 2003
Externally publishedYes

Fingerprint

Inner Ear
Ear
Rhombencephalon
Fibroblast Growth Factors
Genes
Vertebrates
Neomycin
Organogenesis
Homologous Recombination
Zebrafish
Xenopus
Transgenic Mice
Embryonic Development
Alleles

Keywords

  • Fibroblast growth factor
  • Hindbrain
  • Mouse
  • Otic vesicle

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Alvarez, Y., Alonso, M. T., Vendrell, V., Zelarayan, L. C., Chamero, P., Theil, T., ... Schimmang, T. (2003). Requirements for FGF3 and FGF10 during inner ear formation. Development (Cambridge), 130(25), 6329-6338. https://doi.org/10.1242/dev.00881

Requirements for FGF3 and FGF10 during inner ear formation. / Alvarez, Yolanda; Alonso, Maria Teresa; Vendrell, Victor; Zelarayan, Laura Cecilia; Chamero, Pablo; Theil, Thomas; Bösl, Michael R.; Kato, Shigeaki; Maconochie, Mark; Rietmacher, Dieter; Schimmang, Thomas.

In: Development (Cambridge), Vol. 130, No. 25, 12.2003, p. 6329-6338.

Research output: Contribution to journalArticle

Alvarez, Y, Alonso, MT, Vendrell, V, Zelarayan, LC, Chamero, P, Theil, T, Bösl, MR, Kato, S, Maconochie, M, Rietmacher, D & Schimmang, T 2003, 'Requirements for FGF3 and FGF10 during inner ear formation', Development (Cambridge), vol. 130, no. 25, pp. 6329-6338. https://doi.org/10.1242/dev.00881
Alvarez Y, Alonso MT, Vendrell V, Zelarayan LC, Chamero P, Theil T et al. Requirements for FGF3 and FGF10 during inner ear formation. Development (Cambridge). 2003 Dec;130(25):6329-6338. https://doi.org/10.1242/dev.00881
Alvarez, Yolanda ; Alonso, Maria Teresa ; Vendrell, Victor ; Zelarayan, Laura Cecilia ; Chamero, Pablo ; Theil, Thomas ; Bösl, Michael R. ; Kato, Shigeaki ; Maconochie, Mark ; Rietmacher, Dieter ; Schimmang, Thomas. / Requirements for FGF3 and FGF10 during inner ear formation. In: Development (Cambridge). 2003 ; Vol. 130, No. 25. pp. 6329-6338.
@article{3714d4a4cf4145bda9b2c534912938e4,
title = "Requirements for FGF3 and FGF10 during inner ear formation",
abstract = "Members of the fibroblast growth factor (FGF) gene family control formation of the body plan and organogenesis in vertebrates. FGF3 is expressed in the developing hindbrain and has been shown to be involved in inner ear development of different vertebrate species, including zebrafish, Xenopus, chick and mouse. In the mouse, insertion of a neomycin resistance gene into the Fgf3 gene via homologous recombination results in severe developmental defects during differentiation of the otic vesicle. We have addressed the precise roles of FGF3 and other FGF family members during formation of the murine inner ear using both loss- and gain-of-function experiments. We generated a new mutant allele lacking the entire FGF3-coding region but surprisingly found no evidence for severe defects either during inner ear development or in the mature sensory organ, suggesting the functional involvement of other FGF family members during its formation. Ectopic expression of FGF10 in the developing hindbrain of transgenic mice leads to the formation of ectopic vesicles, expressing some otic marker genes and thus indicating a role for FGF10 during otic vesicle formation. Expression analysis of FGF10 during mouse embryogenesis reveals a highly dynamic pattern of expression in the developing hindbrain, partially overlapping with FGF3 expression and coinciding with formation of the inner ear. However, FGF10 mutant mice have been reported to display only mild defects during inner ear differentiation. We thus created double mutant mice for FGF3 and FGF10, which form severely reduced otic vesicles, suggesting redundant roles of these FGFs, acting in combination as neural signals for otic vesicle formation.",
keywords = "Fibroblast growth factor, Hindbrain, Mouse, Otic vesicle",
author = "Yolanda Alvarez and Alonso, {Maria Teresa} and Victor Vendrell and Zelarayan, {Laura Cecilia} and Pablo Chamero and Thomas Theil and B{\"o}sl, {Michael R.} and Shigeaki Kato and Mark Maconochie and Dieter Rietmacher and Thomas Schimmang",
year = "2003",
month = "12",
doi = "10.1242/dev.00881",
language = "English",
volume = "130",
pages = "6329--6338",
journal = "Development",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "25",

}

TY - JOUR

T1 - Requirements for FGF3 and FGF10 during inner ear formation

AU - Alvarez, Yolanda

AU - Alonso, Maria Teresa

AU - Vendrell, Victor

AU - Zelarayan, Laura Cecilia

AU - Chamero, Pablo

AU - Theil, Thomas

AU - Bösl, Michael R.

AU - Kato, Shigeaki

AU - Maconochie, Mark

AU - Rietmacher, Dieter

AU - Schimmang, Thomas

PY - 2003/12

Y1 - 2003/12

N2 - Members of the fibroblast growth factor (FGF) gene family control formation of the body plan and organogenesis in vertebrates. FGF3 is expressed in the developing hindbrain and has been shown to be involved in inner ear development of different vertebrate species, including zebrafish, Xenopus, chick and mouse. In the mouse, insertion of a neomycin resistance gene into the Fgf3 gene via homologous recombination results in severe developmental defects during differentiation of the otic vesicle. We have addressed the precise roles of FGF3 and other FGF family members during formation of the murine inner ear using both loss- and gain-of-function experiments. We generated a new mutant allele lacking the entire FGF3-coding region but surprisingly found no evidence for severe defects either during inner ear development or in the mature sensory organ, suggesting the functional involvement of other FGF family members during its formation. Ectopic expression of FGF10 in the developing hindbrain of transgenic mice leads to the formation of ectopic vesicles, expressing some otic marker genes and thus indicating a role for FGF10 during otic vesicle formation. Expression analysis of FGF10 during mouse embryogenesis reveals a highly dynamic pattern of expression in the developing hindbrain, partially overlapping with FGF3 expression and coinciding with formation of the inner ear. However, FGF10 mutant mice have been reported to display only mild defects during inner ear differentiation. We thus created double mutant mice for FGF3 and FGF10, which form severely reduced otic vesicles, suggesting redundant roles of these FGFs, acting in combination as neural signals for otic vesicle formation.

AB - Members of the fibroblast growth factor (FGF) gene family control formation of the body plan and organogenesis in vertebrates. FGF3 is expressed in the developing hindbrain and has been shown to be involved in inner ear development of different vertebrate species, including zebrafish, Xenopus, chick and mouse. In the mouse, insertion of a neomycin resistance gene into the Fgf3 gene via homologous recombination results in severe developmental defects during differentiation of the otic vesicle. We have addressed the precise roles of FGF3 and other FGF family members during formation of the murine inner ear using both loss- and gain-of-function experiments. We generated a new mutant allele lacking the entire FGF3-coding region but surprisingly found no evidence for severe defects either during inner ear development or in the mature sensory organ, suggesting the functional involvement of other FGF family members during its formation. Ectopic expression of FGF10 in the developing hindbrain of transgenic mice leads to the formation of ectopic vesicles, expressing some otic marker genes and thus indicating a role for FGF10 during otic vesicle formation. Expression analysis of FGF10 during mouse embryogenesis reveals a highly dynamic pattern of expression in the developing hindbrain, partially overlapping with FGF3 expression and coinciding with formation of the inner ear. However, FGF10 mutant mice have been reported to display only mild defects during inner ear differentiation. We thus created double mutant mice for FGF3 and FGF10, which form severely reduced otic vesicles, suggesting redundant roles of these FGFs, acting in combination as neural signals for otic vesicle formation.

KW - Fibroblast growth factor

KW - Hindbrain

KW - Mouse

KW - Otic vesicle

UR - http://www.scopus.com/inward/record.url?scp=10744220359&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744220359&partnerID=8YFLogxK

U2 - 10.1242/dev.00881

DO - 10.1242/dev.00881

M3 - Article

VL - 130

SP - 6329

EP - 6338

JO - Development

JF - Development

SN - 0950-1991

IS - 25

ER -