Restricted clonal expression of IL-2 by naive T cells reflects differential dynamic interactions with dendritic cells

Vincent Hurez, Arman Saparov, Albert Tousson, Michael J. Fuller, Takekazu Kubo, James Oliver, Benjamin T. Weaver, Casey T. Weaver

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendritic cell (DC)-T cell interactions. T cells destined to produce IL-2 required prolonged interactions with DCs, whereas most T cells established only transient interactions with DCs and were activated, but did not express IL-2. Extended conjugation of T cells with DCs was not always sufficient to initiate IL-2 expression. Thus, there is intrinsic variability in clonal T cell populations that restricts IL-2 commitment, and prolonged engagement with mature DCs is necessary, but not sufficient, for IL-2 gene transcription.

Original languageEnglish
Pages (from-to)123-132
Number of pages10
JournalJournal of Experimental Medicine
Volume198
Issue number1
DOIs
Publication statusPublished - Jul 7 2003
Externally publishedYes

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Dendritic Cells
Interleukin-2
T-Lymphocytes
Cell Communication
Population
Genes

Keywords

  • Dendritic cells
  • Interleukin 2
  • Lymphocyte activation
  • T lymphocytes
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology

Cite this

Restricted clonal expression of IL-2 by naive T cells reflects differential dynamic interactions with dendritic cells. / Hurez, Vincent; Saparov, Arman; Tousson, Albert; Fuller, Michael J.; Kubo, Takekazu; Oliver, James; Weaver, Benjamin T.; Weaver, Casey T.

In: Journal of Experimental Medicine, Vol. 198, No. 1, 07.07.2003, p. 123-132.

Research output: Contribution to journalArticle

Hurez, Vincent ; Saparov, Arman ; Tousson, Albert ; Fuller, Michael J. ; Kubo, Takekazu ; Oliver, James ; Weaver, Benjamin T. ; Weaver, Casey T. / Restricted clonal expression of IL-2 by naive T cells reflects differential dynamic interactions with dendritic cells. In: Journal of Experimental Medicine. 2003 ; Vol. 198, No. 1. pp. 123-132.
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AU - Weaver, Benjamin T.

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