TY - JOUR
T1 - Review of COVID-19 testing and diagnostic methods
AU - Filchakova, Olena
AU - Dossym, Dina
AU - Ilyas, Aisha
AU - Kuanysheva, Tamila
AU - Abdizhamil, Altynay
AU - Bukasov, Rostislav
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/7/1
Y1 - 2022/7/1
N2 - More than six billion tests for COVID-19 has been already performed in the world. The testing for SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) virus and corresponding human antibodies is essential not only for diagnostics and treatment of the infection by medical institutions, but also as a pre-requisite for major semi-normal economic and social activities such as international flights, off line work and study in offices, access to malls, sport and social events. Accuracy, sensitivity, specificity, time to results and cost per test are essential parameters of those tests and even minimal improvement in any of them may have noticeable impact on life in the many countries of the world. We described, analyzed and compared methods of COVID-19 detection, while representing their parameters in 22 tables. Also, we compared test performance of some FDA approved test kits with clinical performance of some non-FDA approved methods just described in scientific literature. RT-PCR still remains a golden standard in detection of the virus, but a pressing need for alternative less expensive, more rapid, point of care methods is evident. Those methods that may eventually get developed to satisfy this need are explained, discussed, quantitatively compared. The review has a bioanalytical chemistry prospective, but it may be interesting for a broader circle of readers who are interested in understanding and improvement of COVID-19 testing, helping eventually to leave COVID-19 pandemic in the past.
AB - More than six billion tests for COVID-19 has been already performed in the world. The testing for SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) virus and corresponding human antibodies is essential not only for diagnostics and treatment of the infection by medical institutions, but also as a pre-requisite for major semi-normal economic and social activities such as international flights, off line work and study in offices, access to malls, sport and social events. Accuracy, sensitivity, specificity, time to results and cost per test are essential parameters of those tests and even minimal improvement in any of them may have noticeable impact on life in the many countries of the world. We described, analyzed and compared methods of COVID-19 detection, while representing their parameters in 22 tables. Also, we compared test performance of some FDA approved test kits with clinical performance of some non-FDA approved methods just described in scientific literature. RT-PCR still remains a golden standard in detection of the virus, but a pressing need for alternative less expensive, more rapid, point of care methods is evident. Those methods that may eventually get developed to satisfy this need are explained, discussed, quantitatively compared. The review has a bioanalytical chemistry prospective, but it may be interesting for a broader circle of readers who are interested in understanding and improvement of COVID-19 testing, helping eventually to leave COVID-19 pandemic in the past.
KW - Antibody to SARS-CoV-2
KW - CLIA
KW - COVID-19 tests
KW - CRISPR
KW - CT scanning
KW - ELISA
KW - False negative results
KW - False positive results
KW - LAMP
KW - Lateral flow immunoassays
KW - RT-PCR of SARS CoV-2
KW - Rapid detection
KW - SARS CoV-2
KW - Time to results
KW - Ultrasound scanning
UR - http://www.scopus.com/inward/record.url?scp=85127334440&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85127334440&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2022.123409
DO - 10.1016/j.talanta.2022.123409
M3 - Review article
AN - SCOPUS:85127334440
SN - 0039-9140
VL - 244
JO - Talanta
JF - Talanta
M1 - 123409
ER -