Role of the centrosome in mitosis: UV micro-irradiation study

R. E. Uzbekov, M. S. Votchal, I. A. Vorobjev

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Ultraviolet micro-irradiation (UV-MI) of the PK (pig kidney embryo) cell centrosome (λmax=280 nm, spot diameter 1.6 mm, exposure time 5-15 s) at metaphase and anaphase resulted in functional damage of the centrosome. After UV-MI of the centrosome at early metaphase, chromosomes quickly (in 1-3 min) moved away from the irradiated pole and then encircled the non-irradiated pole. Within 10 min after UV-MI spindle disassembled and chromosomes remained unseparated. The minimal dose inducing this effect in 90% of cells was accumulated in 5 s. After the same UV-MI at late metaphase, chromosomes shifted towards the non-irradiated pole; however, anaphase started and chromosome motion towards the non-irradiated pole continued normally. UV-MI of the centrosome at early anaphase for 5-15 s slowed down and then stopped chromosome motion towards the irradiated pole. This was a result of rapid (within 2-3 min) disorganization of the half-spindle. Chromosomes continued to move towards the opposite pole normally, while cytokinesis was significantly retarded. No visible lesion was revealed by electron microscopy after 5 s UV-MI, while 15 s irradiation resulted in the truncation of the microtubule bundles 1.5-2 μm from the centrosome. We concluded that UV-MI inactivates the centrosome and induces disaggregation of microtubule initiation sites. The critical point (checkpoint) in mitosis up to which this damage induces mitotic arrest is mid-metaphase.

Original languageEnglish
Pages (from-to)163-170
Number of pages8
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume29
Issue number2-3
DOIs
Publication statusPublished - Aug 1995

Keywords

  • Centrosome
  • Micro-irradiation
  • Microtubules
  • Mitosis

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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