Scanning near-field optical microscopy-based study of local dynamics of receptor-ligand interactions at the single molecule level

M. Mensi, K. Dukenbayev, S. K. Sekatskii, G. Dietler

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor-ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. The main advantage of this approach consists in the possibility to study the single molecule interaction dynamics with a subwavelength spatial resolution and a submillisecond time resolution. Additionally, due to the much smaller irradiation area and some other technical features, such a modification enables to enlarge the scope of the receptor-ligand pairs to be investigated and to improve the temporal resolution. We briefly discuss corresponding experimental set up with a special accent on the SNOM operation in liquid and present some preliminary results of related investigations.

Original languageEnglish
Pages (from-to)78-84
Number of pages7
JournalLaser Physics
Volume20
Issue number1
DOIs
Publication statusPublished - Jan 2010
Externally publishedYes

Fingerprint

Near field scanning optical microscopy
optical microscopes
near fields
Microscopes
Ligands
microscopy
Scanning
fluorescence
ligands
Molecules
scanning
Fluorescence microscopy
temporal resolution
molecules
spatial resolution
Fluorescence
Irradiation
interactions
Imaging techniques
irradiation

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Industrial and Manufacturing Engineering

Cite this

Scanning near-field optical microscopy-based study of local dynamics of receptor-ligand interactions at the single molecule level. / Mensi, M.; Dukenbayev, K.; Sekatskii, S. K.; Dietler, G.

In: Laser Physics, Vol. 20, No. 1, 01.2010, p. 78-84.

Research output: Contribution to journalArticle

Mensi, M. ; Dukenbayev, K. ; Sekatskii, S. K. ; Dietler, G. / Scanning near-field optical microscopy-based study of local dynamics of receptor-ligand interactions at the single molecule level. In: Laser Physics. 2010 ; Vol. 20, No. 1. pp. 78-84.
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