Abstract
A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor-ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. The main advantage of this approach consists in the possibility to study the single molecule interaction dynamics with a subwavelength spatial resolution and a submillisecond time resolution. Additionally, due to the much smaller irradiation area and some other technical features, such a modification enables to enlarge the scope of the receptor-ligand pairs to be investigated and to improve the temporal resolution. We briefly discuss corresponding experimental set up with a special accent on the SNOM operation in liquid and present some preliminary results of related investigations.
| Original language | English |
|---|---|
| Pages (from-to) | 78-84 |
| Number of pages | 7 |
| Journal | Laser Physics |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Condensed Matter Physics
- Atomic and Molecular Physics, and Optics
- Instrumentation
- Industrial and Manufacturing Engineering
Cite this
Scanning near-field optical microscopy-based study of local dynamics of receptor-ligand interactions at the single molecule level. / Mensi, M.; Dukenbayev, K.; Sekatskii, S. K.; Dietler, G.
In: Laser Physics, Vol. 20, No. 1, 01.2010, p. 78-84.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Scanning near-field optical microscopy-based study of local dynamics of receptor-ligand interactions at the single molecule level
AU - Mensi, M.
AU - Dukenbayev, K.
AU - Sekatskii, S. K.
AU - Dietler, G.
PY - 2010/1
Y1 - 2010/1
N2 - A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor-ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. The main advantage of this approach consists in the possibility to study the single molecule interaction dynamics with a subwavelength spatial resolution and a submillisecond time resolution. Additionally, due to the much smaller irradiation area and some other technical features, such a modification enables to enlarge the scope of the receptor-ligand pairs to be investigated and to improve the temporal resolution. We briefly discuss corresponding experimental set up with a special accent on the SNOM operation in liquid and present some preliminary results of related investigations.
AB - A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor-ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. The main advantage of this approach consists in the possibility to study the single molecule interaction dynamics with a subwavelength spatial resolution and a submillisecond time resolution. Additionally, due to the much smaller irradiation area and some other technical features, such a modification enables to enlarge the scope of the receptor-ligand pairs to be investigated and to improve the temporal resolution. We briefly discuss corresponding experimental set up with a special accent on the SNOM operation in liquid and present some preliminary results of related investigations.
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UR - http://www.scopus.com/inward/citedby.url?scp=76849099246&partnerID=8YFLogxK
U2 - 10.1134/S1054660X09170125
DO - 10.1134/S1054660X09170125
M3 - Article
VL - 20
SP - 78
EP - 84
JO - Laser Physics
JF - Laser Physics
SN - 1054-660X
IS - 1
ER -