Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice

Maik Dahlhoff, Emanuela Camera, Matthias Schäfer, Daniela Emrich, Dieter Riethmacher, April Foster, Ralf Paus, Marlon R Schneider

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Sebocytes, which are characterized by lipid accumulation that leads to cell disruption, can be found in hair follicle-associated sebaceous glands (SGs) or in free SGs such as the Meibomian glands in the eyelids. Because genetic tools that allow targeting of sebocytes while maintaining intact epidermal lipids are lacking, the relevance of sebaceous lipids in health and disease remains poorly understood. Using Scd3, which is expressed exclusively in mature sebocytes, we established a mouse line with sebocyte-specific expression of Cre recombinase. Both RT-PCR analysis and crossing into Rosa26-lacZ reporter mice and KrasG12D mice confirmed Cre activity specifically in SGs, with no activity in other skin compartments. Importantly, loss of SCD3 function did not cause detectable phenotypical alterations, endorsing the usefulness of Scd3-Cre mice for further functional studies. Scd3-Cre-induced, diphtheria chain A toxin-mediated depletion of sebaceous lipids resulted in impaired water repulsion and thermoregulation, increased rates of UVB-induced epidermal apoptosis and caused a severe pathology of the ocular surface resembling Meibomian gland dysfunction. This novel mouse line will be useful for further investigating the roles of sebaceous lipids in skin and eye integrity.

Original languageEnglish
Pages (from-to)1823-1831
Number of pages9
JournalDevelopment (Cambridge)
Volume143
Issue number10
DOIs
Publication statusPublished - May 15 2016
Externally publishedYes

Keywords

  • Gene targeting
  • Meibomian gland disease
  • Mouse
  • Sebaceous glands
  • Water repulsion

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

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