TY - JOUR
T1 - Sensitive detection of kidney injury biomarker (KIM1) in urine samples using an optical fiber semi-distributed interferometer biosensor
AU - Seitkamal, Kuanysh
AU - Afroz, Anum
AU - Tleuzhanova, Aigerim
AU - Makhammajanov, Zhalaliddin
AU - Kumar, Santosh
AU - Gaipov, Abduzhappar
AU - Blanc, Wilfried
AU - Tosi, Daniele
AU - Bekmurzayeva, Aliya
N1 - Publisher Copyright:
© 2025
PY - 2025/12/1
Y1 - 2025/12/1
N2 - Kidney injury molecule 1 (KIM-1) is a promising biomarker for early diagnosis of various kidney diseases such as acute kidney injury and chronic kidney disease. This study introduces a fiber-optic biosensor based on a semi-distributed interferometer (SDI) designed for direct measurement of KIM-1 in human urine specimens. The biosensor demonstrated exceptional sensitivity and specificity to KIM1 protein, and the KIM-1-functionalized sensor significantly surpassed the control sensors with optimal responses in both artificial and abnormal urine samples. The response of the SDI biosensor to KIM-1 in clinical samples showed a linear range from 10 aM to 1 nM, covering concentrations between 1 aM and 100 nM. The lowest limit of detection is 13.6 aM with an exceptional accuracy. Clinical testing confirmed the biosensor's high selectivity, sensitivity, and stability, positioning it as a promising non-invasive diagnostic tool for early detection and monitoring of kidney diseases.
AB - Kidney injury molecule 1 (KIM-1) is a promising biomarker for early diagnosis of various kidney diseases such as acute kidney injury and chronic kidney disease. This study introduces a fiber-optic biosensor based on a semi-distributed interferometer (SDI) designed for direct measurement of KIM-1 in human urine specimens. The biosensor demonstrated exceptional sensitivity and specificity to KIM1 protein, and the KIM-1-functionalized sensor significantly surpassed the control sensors with optimal responses in both artificial and abnormal urine samples. The response of the SDI biosensor to KIM-1 in clinical samples showed a linear range from 10 aM to 1 nM, covering concentrations between 1 aM and 100 nM. The lowest limit of detection is 13.6 aM with an exceptional accuracy. Clinical testing confirmed the biosensor's high selectivity, sensitivity, and stability, positioning it as a promising non-invasive diagnostic tool for early detection and monitoring of kidney diseases.
KW - Kidney diseases
KW - KIM1 detection
KW - Optical fiber biosensors
KW - Semi-distributed interferometer
KW - Surface functionalization
KW - Urine-based diagnostics
UR - https://www.scopus.com/pages/publications/105005178782
UR - https://www.scopus.com/pages/publications/105005178782#tab=citedBy
U2 - 10.1016/j.talanta.2025.128348
DO - 10.1016/j.talanta.2025.128348
M3 - Article
AN - SCOPUS:105005178782
SN - 0039-9140
VL - 295
JO - Talanta
JF - Talanta
M1 - 128348
ER -
