Sertoli cells control peritubular myoid cell fate and support adult leydig cell development in the prepubertal testis

Diane Rebourcet, Peter J. O'Shaughnessy, Jean Luc Pitetti, Ana Monteiro, Laura O'Hara, Laura Milne, Yi Ting Tsai, Lyndsey Cruickshanks, Dieter Riethmacher, Florian Guillou, Rod T. Mitchell, Rob van't Hof, Tom C. Freeman, Serge Nef, Lee B. Smith

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Sertoli cells (SCs) regulate testicular fate in the differentiating gonad and are the main regulators of spermatogenesis in the adult testis; however, their role during the intervening period of testis development, in particular during adult Leydig cell (ALC) differentiation and function, remains largely unknown. To examine SC function during fetal and prepubertal development we generated two transgenic mouse models that permit controlled, cell-specific ablation of SCs in pre-and postnatal life. Results show that SCs are required: (1) to maintain the differentiated phenotype of peritubular myoid cells (PTMCs) in prepubertal life; (2) to maintain the ALC progenitor population in the postnatal testis; and (3) for development of normal ALC numbers. Furthermore, our data show that fetal LCs function independently from SC, germ cell or PTMC support in the prepubertal testis. Together, these findings reveal that SCs remain essential regulators of testis development long after the period of sex determination. These findings have significant implications for our understanding of male reproductive disorders and wider androgen-related conditions affecting male health.

Original languageEnglish
Pages (from-to)2139-2149
Number of pages11
JournalDevelopment (Cambridge)
Volume141
Issue number10
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Leydig Cells
Sertoli Cells
Testis
Gonads
Spermatogenesis
Fetal Development
Germ Cells
Transgenic Mice
Androgens
Cell Differentiation
Cell Count
Phenotype
Health
Population

Keywords

  • Diphtheria
  • Leydig
  • Male fertility
  • Mouse
  • Peritubular myoid
  • Sertoli
  • Testis

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Rebourcet, D., O'Shaughnessy, P. J., Pitetti, J. L., Monteiro, A., O'Hara, L., Milne, L., ... Smith, L. B. (2014). Sertoli cells control peritubular myoid cell fate and support adult leydig cell development in the prepubertal testis. Development (Cambridge), 141(10), 2139-2149. https://doi.org/10.1242/dev.107029

Sertoli cells control peritubular myoid cell fate and support adult leydig cell development in the prepubertal testis. / Rebourcet, Diane; O'Shaughnessy, Peter J.; Pitetti, Jean Luc; Monteiro, Ana; O'Hara, Laura; Milne, Laura; Tsai, Yi Ting; Cruickshanks, Lyndsey; Riethmacher, Dieter; Guillou, Florian; Mitchell, Rod T.; van't Hof, Rob; Freeman, Tom C.; Nef, Serge; Smith, Lee B.

In: Development (Cambridge), Vol. 141, No. 10, 2014, p. 2139-2149.

Research output: Contribution to journalArticle

Rebourcet, D, O'Shaughnessy, PJ, Pitetti, JL, Monteiro, A, O'Hara, L, Milne, L, Tsai, YT, Cruickshanks, L, Riethmacher, D, Guillou, F, Mitchell, RT, van't Hof, R, Freeman, TC, Nef, S & Smith, LB 2014, 'Sertoli cells control peritubular myoid cell fate and support adult leydig cell development in the prepubertal testis', Development (Cambridge), vol. 141, no. 10, pp. 2139-2149. https://doi.org/10.1242/dev.107029
Rebourcet, Diane ; O'Shaughnessy, Peter J. ; Pitetti, Jean Luc ; Monteiro, Ana ; O'Hara, Laura ; Milne, Laura ; Tsai, Yi Ting ; Cruickshanks, Lyndsey ; Riethmacher, Dieter ; Guillou, Florian ; Mitchell, Rod T. ; van't Hof, Rob ; Freeman, Tom C. ; Nef, Serge ; Smith, Lee B. / Sertoli cells control peritubular myoid cell fate and support adult leydig cell development in the prepubertal testis. In: Development (Cambridge). 2014 ; Vol. 141, No. 10. pp. 2139-2149.
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abstract = "Sertoli cells (SCs) regulate testicular fate in the differentiating gonad and are the main regulators of spermatogenesis in the adult testis; however, their role during the intervening period of testis development, in particular during adult Leydig cell (ALC) differentiation and function, remains largely unknown. To examine SC function during fetal and prepubertal development we generated two transgenic mouse models that permit controlled, cell-specific ablation of SCs in pre-and postnatal life. Results show that SCs are required: (1) to maintain the differentiated phenotype of peritubular myoid cells (PTMCs) in prepubertal life; (2) to maintain the ALC progenitor population in the postnatal testis; and (3) for development of normal ALC numbers. Furthermore, our data show that fetal LCs function independently from SC, germ cell or PTMC support in the prepubertal testis. Together, these findings reveal that SCs remain essential regulators of testis development long after the period of sex determination. These findings have significant implications for our understanding of male reproductive disorders and wider androgen-related conditions affecting male health.",
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