Silver development in microscopy and bioanalysis: A new versatile formulation for modern needs

Geoffrey R. Newman, Bharat Jasani

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Potentially, silver development could unify most modern demands for clean, accurately localized marker amplification in microscopy and bioanalysis. However, the existing technology leaves room for improvement in developer design. A new formulation has been devised which, by using principles of silver chelation, avoids problems of self-nucleation and catalysis by light. It is made, just before use, by mixing together equal amounts of stock solutions containing high molarity, Tris-buffered silver nitrate and alcoholic, buffered pyrogallol. The two stocks are easily prepared and have very long shelf-lives. The developer is light insensitive for up to an hour at room temperature, so that development can proceed under ambient light conditions and at the neutral pH most suited to biological systems. The powerful reducer in the suggested formulation should allow the detection of low concentrations of marker signal in a wide range of applications.

Original languageEnglish
Pages (from-to)635-645
Number of pages11
JournalThe Histochemical Journal
Volume30
Issue number9
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Silver
Microscopy
Light
Pyrogallol
Silver Nitrate
Catalysis
Technology
Temperature

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Silver development in microscopy and bioanalysis : A new versatile formulation for modern needs. / Newman, Geoffrey R.; Jasani, Bharat.

In: The Histochemical Journal, Vol. 30, No. 9, 1998, p. 635-645.

Research output: Contribution to journalArticle

Newman, Geoffrey R. ; Jasani, Bharat. / Silver development in microscopy and bioanalysis : A new versatile formulation for modern needs. In: The Histochemical Journal. 1998 ; Vol. 30, No. 9. pp. 635-645.
@article{141b90aa2df948d390baac387cbd35d1,
title = "Silver development in microscopy and bioanalysis: A new versatile formulation for modern needs",
abstract = "Potentially, silver development could unify most modern demands for clean, accurately localized marker amplification in microscopy and bioanalysis. However, the existing technology leaves room for improvement in developer design. A new formulation has been devised which, by using principles of silver chelation, avoids problems of self-nucleation and catalysis by light. It is made, just before use, by mixing together equal amounts of stock solutions containing high molarity, Tris-buffered silver nitrate and alcoholic, buffered pyrogallol. The two stocks are easily prepared and have very long shelf-lives. The developer is light insensitive for up to an hour at room temperature, so that development can proceed under ambient light conditions and at the neutral pH most suited to biological systems. The powerful reducer in the suggested formulation should allow the detection of low concentrations of marker signal in a wide range of applications.",
author = "Newman, {Geoffrey R.} and Bharat Jasani",
year = "1998",
doi = "10.1023/A:1003404128497",
language = "English",
volume = "30",
pages = "635--645",
journal = "Journal of Molecular Histology",
issn = "1567-2379",
publisher = "Springer Netherlands",
number = "9",

}

TY - JOUR

T1 - Silver development in microscopy and bioanalysis

T2 - A new versatile formulation for modern needs

AU - Newman, Geoffrey R.

AU - Jasani, Bharat

PY - 1998

Y1 - 1998

N2 - Potentially, silver development could unify most modern demands for clean, accurately localized marker amplification in microscopy and bioanalysis. However, the existing technology leaves room for improvement in developer design. A new formulation has been devised which, by using principles of silver chelation, avoids problems of self-nucleation and catalysis by light. It is made, just before use, by mixing together equal amounts of stock solutions containing high molarity, Tris-buffered silver nitrate and alcoholic, buffered pyrogallol. The two stocks are easily prepared and have very long shelf-lives. The developer is light insensitive for up to an hour at room temperature, so that development can proceed under ambient light conditions and at the neutral pH most suited to biological systems. The powerful reducer in the suggested formulation should allow the detection of low concentrations of marker signal in a wide range of applications.

AB - Potentially, silver development could unify most modern demands for clean, accurately localized marker amplification in microscopy and bioanalysis. However, the existing technology leaves room for improvement in developer design. A new formulation has been devised which, by using principles of silver chelation, avoids problems of self-nucleation and catalysis by light. It is made, just before use, by mixing together equal amounts of stock solutions containing high molarity, Tris-buffered silver nitrate and alcoholic, buffered pyrogallol. The two stocks are easily prepared and have very long shelf-lives. The developer is light insensitive for up to an hour at room temperature, so that development can proceed under ambient light conditions and at the neutral pH most suited to biological systems. The powerful reducer in the suggested formulation should allow the detection of low concentrations of marker signal in a wide range of applications.

UR - http://www.scopus.com/inward/record.url?scp=0031796830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031796830&partnerID=8YFLogxK

U2 - 10.1023/A:1003404128497

DO - 10.1023/A:1003404128497

M3 - Article

C2 - 9870764

AN - SCOPUS:0031796830

VL - 30

SP - 635

EP - 645

JO - Journal of Molecular Histology

JF - Journal of Molecular Histology

SN - 1567-2379

IS - 9

ER -