Abstract
Potentially, silver development could unify most modern demands for clean, accurately localized marker amplification in microscopy and bioanalysis. However, the existing technology leaves room for improvement in developer design. A new formulation has been devised which, by using principles of silver chelation, avoids problems of self-nucleation and catalysis by light. It is made, just before use, by mixing together equal amounts of stock solutions containing high molarity, Tris-buffered silver nitrate and alcoholic, buffered pyrogallol. The two stocks are easily prepared and have very long shelf-lives. The developer is light insensitive for up to an hour at room temperature, so that development can proceed under ambient light conditions and at the neutral pH most suited to biological systems. The powerful reducer in the suggested formulation should allow the detection of low concentrations of marker signal in a wide range of applications.
| Original language | English |
|---|---|
| Pages (from-to) | 635-645 |
| Number of pages | 11 |
| Journal | The Histochemical Journal |
| Volume | 30 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 1998 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
Silver development in microscopy and bioanalysis : A new versatile formulation for modern needs. / Newman, Geoffrey R.; Jasani, Bharat.
In: The Histochemical Journal, Vol. 30, No. 9, 1998, p. 635-645.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Silver development in microscopy and bioanalysis
T2 - A new versatile formulation for modern needs
AU - Newman, Geoffrey R.
AU - Jasani, Bharat
PY - 1998
Y1 - 1998
N2 - Potentially, silver development could unify most modern demands for clean, accurately localized marker amplification in microscopy and bioanalysis. However, the existing technology leaves room for improvement in developer design. A new formulation has been devised which, by using principles of silver chelation, avoids problems of self-nucleation and catalysis by light. It is made, just before use, by mixing together equal amounts of stock solutions containing high molarity, Tris-buffered silver nitrate and alcoholic, buffered pyrogallol. The two stocks are easily prepared and have very long shelf-lives. The developer is light insensitive for up to an hour at room temperature, so that development can proceed under ambient light conditions and at the neutral pH most suited to biological systems. The powerful reducer in the suggested formulation should allow the detection of low concentrations of marker signal in a wide range of applications.
AB - Potentially, silver development could unify most modern demands for clean, accurately localized marker amplification in microscopy and bioanalysis. However, the existing technology leaves room for improvement in developer design. A new formulation has been devised which, by using principles of silver chelation, avoids problems of self-nucleation and catalysis by light. It is made, just before use, by mixing together equal amounts of stock solutions containing high molarity, Tris-buffered silver nitrate and alcoholic, buffered pyrogallol. The two stocks are easily prepared and have very long shelf-lives. The developer is light insensitive for up to an hour at room temperature, so that development can proceed under ambient light conditions and at the neutral pH most suited to biological systems. The powerful reducer in the suggested formulation should allow the detection of low concentrations of marker signal in a wide range of applications.
UR - http://www.scopus.com/inward/record.url?scp=0031796830&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031796830&partnerID=8YFLogxK
U2 - 10.1023/A:1003404128497
DO - 10.1023/A:1003404128497
M3 - Article
C2 - 9870764
AN - SCOPUS:0031796830
VL - 30
SP - 635
EP - 645
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
SN - 1567-2379
IS - 9
ER -