Simian virus 40 large T antigen's association with the CUL7 SCF complex contributes to cellular transformation

Jocelyn S. Kasper, Hiroshi Kuwabara, Takehiro Arai, Syed Hamid Ali, James A. DeCaprio

Research output: Contribution to journalArticle

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Abstract

Simian virus 40 large T antigen (T Ag) is capable of immortalizing and transforming rodent cells. The transforming activity of T Ag is due in large part to perturbation of the tumor suppressor proteins p53 and the retinoblastoma (pRB) family members. Inactivation of these tumor suppressors may not be sufficient for T Ag-mediated cellular transformation. It has been shown that T Ag associates with an SCF-like complex that contains a member of the cullin family of E3 ubiquitin ligases, CUL7, as well as SKP1, RBX1, and an F-box protein, FBXW8. We identified T Ag residues 69 to 83 as required for T Ag binding to the CUL7 complex. We demonstrate that Δ69-83 T Ag, while it lost its ability to associate with CUL7, retained binding to p53 and pRB family members. In the presence of CUL7, wild-type (WT) T Ag but not Δ69-83 T Ag was able to induce proliferation of mouse embryo fibroblasts, an indication of cellular transformation. In contrast, WT and Δ69-83 T Ag enabled mouse embryo fibroblasts to proliferate to similarly high densities in the absence of CUL7. Our data suggest that, in addition to p53 and the pRB family members, T Ag serves to bind to and inactivate the growth-suppressing properties of CUL7. In addition, these results imply that, at least in the presence of T Ag, CUL7 may function as a tumor suppressor.

Original languageEnglish
Pages (from-to)11685-11692
Number of pages8
JournalJournal of Virology
Volume79
Issue number18
DOIs
Publication statusPublished - Sep 2005
Externally publishedYes

Fingerprint

Simian virus 40
Viral Tumor Antigens
antigens
Retinoblastoma
fibroblasts
embryo (animal)
Embryonic Structures
Fibroblasts
Cullin Proteins
F-Box Proteins
F-box proteins
Tumor Suppressor Protein p53
ubiquitin-protein ligase
neoplasms
Aptitude
Ubiquitin-Protein Ligases
mice

ASJC Scopus subject areas

  • Immunology

Cite this

Simian virus 40 large T antigen's association with the CUL7 SCF complex contributes to cellular transformation. / Kasper, Jocelyn S.; Kuwabara, Hiroshi; Arai, Takehiro; Ali, Syed Hamid; DeCaprio, James A.

In: Journal of Virology, Vol. 79, No. 18, 09.2005, p. 11685-11692.

Research output: Contribution to journalArticle

Kasper, Jocelyn S. ; Kuwabara, Hiroshi ; Arai, Takehiro ; Ali, Syed Hamid ; DeCaprio, James A. / Simian virus 40 large T antigen's association with the CUL7 SCF complex contributes to cellular transformation. In: Journal of Virology. 2005 ; Vol. 79, No. 18. pp. 11685-11692.
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