Abstract
Development of distinct CD4+ T cell cytokine phenotypes may be conditioned by the anatomic site in which activation occurs. A double-label in situ hybridization technique was used to characterize co-expression of cytokine mRNA in antigen-specific responses of Peyer's patch (PP), lamina propria (LP), and splenic (SP) CD4+ T cells isolated from αβ T cell receptor-transgenic mice. Interleukin (IL)-2 was the dominant cytokine expressed by antigen-stimulated PP and SP populations, though it was expressed by a minority of the activated T cells. Cells that expressed interferon (IFN)-γ were less frequent, and IL-4, IL-5, and IL-10 were infrequent. In contrast, cells that expressed IFN-γ or IL-10 were most frequent in the LP population, with lower frequencies of IL-2, and few IL-4- and IL-5-positive cells. Co-expression of two cytokines by the same cell was the exception, regardless of the anatomic site from which the T cells were isolated. The surface phenotype of transgene-positive T cells isolated from each anatomic site was distinct, despite the absence of in vivo exposure to antigen for which the transgenic T cell receptor is specific. These data suggest that the cytokine responses of CD4+ T cells may be conditioned by the microenvironment, independently of specific antigen, and that the LP CD4+ T population has a distinct cytokine expression pattern with counter-regulatory properties that may be important for homeostasis in mucosal immune tissues.
| Original language | English |
|---|---|
| Pages (from-to) | 1774-1781 |
| Number of pages | 8 |
| Journal | European Journal of Immunology |
| Volume | 27 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1997 |
| Externally published | Yes |
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Keywords
- CD4 T cell
- Cytokine
- In situ hybridization
- Mucosal immunity
- T cell receptor-transgenic mouse
ASJC Scopus subject areas
- Immunology
Cite this
Single-cell analyses of CD4+ T cells from αβ T cell receptor-transgenic mice : A distinct mucosal cytokine phenotype in the absence of transgene-specific antigen. / Saparov, Arman; Elson, Charles O.; Devore-Carter, Denise; Bucy, R. Pat; Weaver, Casey T.
In: European Journal of Immunology, Vol. 27, No. 7, 07.1997, p. 1774-1781.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single-cell analyses of CD4+ T cells from αβ T cell receptor-transgenic mice
T2 - A distinct mucosal cytokine phenotype in the absence of transgene-specific antigen
AU - Saparov, Arman
AU - Elson, Charles O.
AU - Devore-Carter, Denise
AU - Bucy, R. Pat
AU - Weaver, Casey T.
PY - 1997/7
Y1 - 1997/7
N2 - Development of distinct CD4+ T cell cytokine phenotypes may be conditioned by the anatomic site in which activation occurs. A double-label in situ hybridization technique was used to characterize co-expression of cytokine mRNA in antigen-specific responses of Peyer's patch (PP), lamina propria (LP), and splenic (SP) CD4+ T cells isolated from αβ T cell receptor-transgenic mice. Interleukin (IL)-2 was the dominant cytokine expressed by antigen-stimulated PP and SP populations, though it was expressed by a minority of the activated T cells. Cells that expressed interferon (IFN)-γ were less frequent, and IL-4, IL-5, and IL-10 were infrequent. In contrast, cells that expressed IFN-γ or IL-10 were most frequent in the LP population, with lower frequencies of IL-2, and few IL-4- and IL-5-positive cells. Co-expression of two cytokines by the same cell was the exception, regardless of the anatomic site from which the T cells were isolated. The surface phenotype of transgene-positive T cells isolated from each anatomic site was distinct, despite the absence of in vivo exposure to antigen for which the transgenic T cell receptor is specific. These data suggest that the cytokine responses of CD4+ T cells may be conditioned by the microenvironment, independently of specific antigen, and that the LP CD4+ T population has a distinct cytokine expression pattern with counter-regulatory properties that may be important for homeostasis in mucosal immune tissues.
AB - Development of distinct CD4+ T cell cytokine phenotypes may be conditioned by the anatomic site in which activation occurs. A double-label in situ hybridization technique was used to characterize co-expression of cytokine mRNA in antigen-specific responses of Peyer's patch (PP), lamina propria (LP), and splenic (SP) CD4+ T cells isolated from αβ T cell receptor-transgenic mice. Interleukin (IL)-2 was the dominant cytokine expressed by antigen-stimulated PP and SP populations, though it was expressed by a minority of the activated T cells. Cells that expressed interferon (IFN)-γ were less frequent, and IL-4, IL-5, and IL-10 were infrequent. In contrast, cells that expressed IFN-γ or IL-10 were most frequent in the LP population, with lower frequencies of IL-2, and few IL-4- and IL-5-positive cells. Co-expression of two cytokines by the same cell was the exception, regardless of the anatomic site from which the T cells were isolated. The surface phenotype of transgene-positive T cells isolated from each anatomic site was distinct, despite the absence of in vivo exposure to antigen for which the transgenic T cell receptor is specific. These data suggest that the cytokine responses of CD4+ T cells may be conditioned by the microenvironment, independently of specific antigen, and that the LP CD4+ T population has a distinct cytokine expression pattern with counter-regulatory properties that may be important for homeostasis in mucosal immune tissues.
KW - CD4 T cell
KW - Cytokine
KW - In situ hybridization
KW - Mucosal immunity
KW - T cell receptor-transgenic mouse
UR - http://www.scopus.com/inward/record.url?scp=0030842853&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030842853&partnerID=8YFLogxK
U2 - 10.1002/eji.1830270727
DO - 10.1002/eji.1830270727
M3 - Article
VL - 27
SP - 1774
EP - 1781
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 7
ER -