Single-cell analyses of CD4+ T cells from αβ T cell receptor-transgenic mice: A distinct mucosal cytokine phenotype in the absence of transgene-specific antigen

Arman Saparov, Charles O. Elson, Denise Devore-Carter, R. Pat Bucy, Casey T. Weaver

Research output: Research - peer-reviewArticle

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Abstract

Development of distinct CD4+ T cell cytokine phenotypes may be conditioned by the anatomic site in which activation occurs. A double-label in situ hybridization technique was used to characterize co-expression of cytokine mRNA in antigen-specific responses of Peyer's patch (PP), lamina propria (LP), and splenic (SP) CD4+ T cells isolated from αβ T cell receptor-transgenic mice. Interleukin (IL)-2 was the dominant cytokine expressed by antigen-stimulated PP and SP populations, though it was expressed by a minority of the activated T cells. Cells that expressed interferon (IFN)-γ were less frequent, and IL-4, IL-5, and IL-10 were infrequent. In contrast, cells that expressed IFN-γ or IL-10 were most frequent in the LP population, with lower frequencies of IL-2, and few IL-4- and IL-5-positive cells. Co-expression of two cytokines by the same cell was the exception, regardless of the anatomic site from which the T cells were isolated. The surface phenotype of transgene-positive T cells isolated from each anatomic site was distinct, despite the absence of in vivo exposure to antigen for which the transgenic T cell receptor is specific. These data suggest that the cytokine responses of CD4+ T cells may be conditioned by the microenvironment, independently of specific antigen, and that the LP CD4+ T population has a distinct cytokine expression pattern with counter-regulatory properties that may be important for homeostasis in mucosal immune tissues.

LanguageEnglish
Pages1774-1781
Number of pages8
JournalEuropean Journal of Immunology
Volume27
Issue number7
DOIs
StatePublished - Jul 1997
Externally publishedYes

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Single-Cell Analysis
T-Cell Antigen Receptor
Transgenes
Transgenic Mice
Cytokines
T-Lymphocytes
Phenotype
Antigens
Mucous Membrane
Population
Peyer's Patches
Interleukin-5
Interleukin-4
Interleukin-10
Interferon-gamma
Interleukin-2
In Situ Hybridization
Homeostasis
Messenger RNA

Keywords

  • CD4 T cell
  • Cytokine
  • In situ hybridization
  • Mucosal immunity
  • T cell receptor-transgenic mouse

ASJC Scopus subject areas

  • Immunology

Cite this

Single-cell analyses of CD4+ T cells from αβ T cell receptor-transgenic mice : A distinct mucosal cytokine phenotype in the absence of transgene-specific antigen. / Saparov, Arman; Elson, Charles O.; Devore-Carter, Denise; Bucy, R. Pat; Weaver, Casey T.

In: European Journal of Immunology, Vol. 27, No. 7, 07.1997, p. 1774-1781.

Research output: Research - peer-reviewArticle

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