SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase

Babet van der Vaart, Cristina Manatschal, Ilya Grigoriev, Vincent Olieric, Susana Montenegro Gouveia, Saša Bjelić, Jeroen Demmers, Ivan Vorobjev, Casper C. Hoogenraad, Michel O. Steinmetz, Anna Akhmanova

Research output: Contribution to journalArticle

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Abstract

The ends of growing microtubules (MTs) accumulate a set of diverse factors known as MT plus end-tracking proteins (+TIPs), which control microtubule dynamics and organization. In this paper, we identify SLAIN2 as a key component of +TIP interaction networks. We showed that the C-terminal part of SLAIN2 bound to end-binding proteins (EBs), cytoplasmic linker proteins (CLIPs), and CLIP-associated proteins and characterized in detail the interaction of SLAIN2 with EB1 and CLIP-170. Furthermore, we found that the N-terminal part of SLAIN2 interacted with ch-TOG, the mammalian homologue of the MT polymerase XMAP215. Through its multiple interactions, SLAIN2 enhanced ch-TOG accumulation at MT plus ends and, as a consequence, strongly stimulated processive MT polymerization in interphase cells. Depletion or disruption of the SLAIN2-ch-TOG complex led to disorganization of the radial MT array. During mitosis, SLAIN2 became highly phosphorylated, and its interaction with EBs and ch-TOG was inhibited. Our study provides new insights into the molecular mechanisms underlying cell cycle-specific regulation of MT polymerization and the organization of the MT network.

Original languageEnglish
Pages (from-to)1083-1099
Number of pages17
JournalJournal of Cell Biology
Volume193
Issue number6
DOIs
Publication statusPublished - Jun 13 2011
Externally publishedYes

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Microtubule Proteins
Interphase
Microtubules
Growth
Polymerization
Proteins
Mitosis
Cell Cycle
Carrier Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

van der Vaart, B., Manatschal, C., Grigoriev, I., Olieric, V., Gouveia, S. M., Bjelić, S., ... Akhmanova, A. (2011). SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase. Journal of Cell Biology, 193(6), 1083-1099. https://doi.org/10.1083/jcb.201012179

SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase. / van der Vaart, Babet; Manatschal, Cristina; Grigoriev, Ilya; Olieric, Vincent; Gouveia, Susana Montenegro; Bjelić, Saša; Demmers, Jeroen; Vorobjev, Ivan; Hoogenraad, Casper C.; Steinmetz, Michel O.; Akhmanova, Anna.

In: Journal of Cell Biology, Vol. 193, No. 6, 13.06.2011, p. 1083-1099.

Research output: Contribution to journalArticle

van der Vaart, B, Manatschal, C, Grigoriev, I, Olieric, V, Gouveia, SM, Bjelić, S, Demmers, J, Vorobjev, I, Hoogenraad, CC, Steinmetz, MO & Akhmanova, A 2011, 'SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase', Journal of Cell Biology, vol. 193, no. 6, pp. 1083-1099. https://doi.org/10.1083/jcb.201012179
van der Vaart B, Manatschal C, Grigoriev I, Olieric V, Gouveia SM, Bjelić S et al. SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase. Journal of Cell Biology. 2011 Jun 13;193(6):1083-1099. https://doi.org/10.1083/jcb.201012179
van der Vaart, Babet ; Manatschal, Cristina ; Grigoriev, Ilya ; Olieric, Vincent ; Gouveia, Susana Montenegro ; Bjelić, Saša ; Demmers, Jeroen ; Vorobjev, Ivan ; Hoogenraad, Casper C. ; Steinmetz, Michel O. ; Akhmanova, Anna. / SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase. In: Journal of Cell Biology. 2011 ; Vol. 193, No. 6. pp. 1083-1099.
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